Human Tumours grown in Mice

Je, Castro

doi:10.1038/newbio239083a0

Nature New Biology

1972

DOI: 10.1038/newbio239083a0

|View full text |Cite

Human Tumours grown in Mice

Castro Je

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1973

2006

Publication Types

Select...

Article10

Relationship

Self Cite0

Independent10

Authors

Journals

Cited by 46 publications

(17 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…THE development of xenografting techniques, enabling human tumours to be grown in immune-suppressed mice (as shown for example by Castro, 1972, andCobb, 1973), has made it possible to experiment in the laboratory on tumours of human origin, and to study the effect on them of the cytotoxic agents used in cancer therapy.…”

mentioning

confidence: 99%

An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents

Courtenay¹,

Mills²

1978

Br J Cancer

270

108

View full text Add to dashboard Cite

Summary.-An in vitro agar colony technique has been developed for the growth of tumour cells taken directly from human tumours grown in immune-suppressed mice. The novel feature of the technique is the addition of a replenishable liquid phase which permits the maintenance of relatively slowly growing cells. A number of different xenografted tumours have been cultured successfully in this system, with red blood cells added to the agar and using 5% 02 in the gas phase.The technique has been used to assay cell survival in tumours treated in vivo with cytotoxic agents, and examples are given of survival curves obtained from a pancreatic tumour irradiated with y-rays and a colonic tumour from mice treated with cyclophosphamide.The results obtained by this in vitro method are in agreement with those from the agar diffusion chamber technique.This culture method has also been successfully used for the growth of cells taken directly from human tumour biopsy samples obtained in the clinic.

show abstract

mentioning

confidence: 99%

An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents

Courtenay¹,

Mills²

1978

Br J Cancer

270

108

View full text Add to dashboard Cite

show abstract

“…CARCINOMA arising in the large bowel in man can be transplanted successfully subcutaneously in immune deprived animals (Povlsen and Rygaard, 1971;Castro, 1972;Cobb, 1972). If we wish to use this xenograft model to learn more about a tumour in man, we need to understand to what extent transplantation to a xenogeneic host alters the tumour cell population.…”

mentioning

confidence: 99%

The Behaviour of Carcinoma of the Large Bowel in Man Following Transplantation into Immune Deprived Mice

Lm¹

1973

Br J Cancer

View full text Add to dashboard Cite

Summary.-The growth of carcinoma of the human large bowel was studied in the first 2 passages in immune deprived mice. The tumours were obtained from large bowel resections on 3 people. There was a strong histological similarity between the patient's tumour and the tumour that grew subcutaneously in the mice 2-8 months after implantation. One dissimilarity observed was a higher mitotic index in some of the tumours growing in the immune deprived animals. In the second passage of the bowel tumours, cells were implanted into groups of 8-10 animals in the following sites: subcutaneous, intramuscular, intravenous, intrahepatic, intraperitoneal and intrathoracic. Growth of tumour was observed from all 3 tumours when they were implanted subcutaneously, intramuscularly, intraperitoneally and intrathoracically. Infiltration of muscle by tumour was a frequent finding. Lung metastases developed after intravenous injection of cells in 1 of the 3 tumours. In none of the 3 tumours did growth follow injection of cells directly into the substance of the liver. On no occasions were spontaneous metastases observed.

show abstract

“…Following the demonstration by Rygaard and Povlsen (1969) that human tumours could be grown in congenitally athymic mice and the subsequent use of thymectomised, whole-body irradiated mice suitable for tumour heterotransplantation (Castro, 1972), large numbers of serially transplantable human tumour xenograft lines have been established (Shimosato et al, 1976Giovanella et al, 1978). In spite of extensive data on the establishment, biological characteristics and drug response of these xenografts, their clinical relevance is not yet clear.…”

mentioning

confidence: 99%

Limitations of the human tumour xenograft system in individual patient drug sensitivity testing

Bailey¹,

Jones²,

Shorthouse³

et al. 1984

Br J Cancer

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Human Tumours grown in Mice

Cited by 46 publications

References 2 publications

An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents

An in vitro colony assay for human tumours grown in immune-suppressed mice and treated in vivo with cytotoxic agents

The Behaviour of Carcinoma of the Large Bowel in Man Following Transplantation into Immune Deprived Mice

Limitations of the human tumour xenograft system in individual patient drug sensitivity testing

Contact Info

Product

Resources

About