Human U1 small nuclear RNA genes: extensive conservation of flanking sequences suggests cycles of gene amplification and transposition.

Bernstein, L B; Manser, Tim; Weiner, Alan M.

doi:10.1128/mcb.5.9.2159

Cited by 60 publications

(38 citation statements)

References 66 publications

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“…The control primers hybridized to a region 4 kb upstream of the U6 transcription start site. The U1 snRNA genes number an estimated 30-100 copies per haploid genome, each carried on a tandemly repeated 45-kb fragment (Bernstein et al 1985). In addition, the human genome contains a large number of U1 pseudogenes.…”

Section: Hbјј Is Found On the U6 Promoter Region In Vivomentioning

confidence: 99%

Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters

Schramm¹,

Pendergrast²,

Sun³

et al. 2000

Genes Dev.

124

186

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. Saccharomyces cerevisiae TFIIIB is a complex of three subunits, TBP, the TFIIB-related factor BRF, and the more loosely associated polypeptide ␤؆. Although human homologs for two of the TFIIIB subunits, the TATA box-binding protein TBP and the TFIIB-related factor BRF, have been characterized, a human homolog of yeast B؆ has not been described. Moreover, human BRF, unlike yeast BRF, is not universally required for RNA polymerase III transcription. In particular, it is not involved in transcription from the small nuclear RNA (snRNA)-type, TATA-containing, RNA polymerase III promoters. Here, we characterize two novel activities, a human homolog of yeast B؆, which is required for transcription of both TATA-less and snRNA-type RNA polymerase III promoters, and a factor equally related to human BRF and TFIIB, designated BRFU, which is specifically required for transcription of snRNA-type RNA polymerase III promoters. Together, these results contribute to the definition of the basal RNA polymerase III transcription machinery and show that two types of TFIIIB activities, with specificities for different classes of RNA polymerase III promoters, have evolved in human cells.

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Section: Hbјј Is Found On the U6 Promoter Region In Vivomentioning

confidence: 99%

Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters

Schramm¹,

Pendergrast²,

Sun³

et al. 2000

Genes Dev.

124

186

View full text Add to dashboard Cite

show abstract

“…In birds and mammals the genes are not present in an obvious repeating unit (1)(2)(3), although the possibility of a large repeated unit cannot be ruled out for humans since the genes isolated thus far are virtually identical in both the 5' and 3' flanking regions (4). The human U2 genes are organized in a large repeated unit (7).…”

Section: Introductionmentioning

confidence: 99%

“…Multiple copies of the Ul RNA gene are present in all organisms in which these genes have been studied (1)(2)(3)(4)(5)(6). In birds and mammals the genes are not present in an obvious repeating unit (1)(2)(3), although the possibility of a large repeated unit cannot be ruled out for humans since the genes isolated thus far are virtually identical in both the 5' and 3' flanking regions (4).…”

Section: Introductionmentioning

confidence: 99%

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Structure and expression of a second sea urchin U1 RNA gene repeat

Nash

Santiago

et al. 1986

Nucl Acids Res

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“…U1 snRNA genes are conserved genes present in eukaryotes and involved in the processing of the primary transcripts in the nucleus. They have been reported to be a complex consisting of tandemly repeated units, as in sea urchins (Brown et al, 1985) and taenids (Bretagne et al, 1991) or consisting of linked but not tandemly repeated units, as in mice (Marzluff et al, 1983) and humans (Bernstein et al, 1985). Possibly, the isolated sequence corresponds to a sequence that degenerated from an ancient U1 snRNA gene or pseudogene in the A. longirostri genome.…”

Section: Discussionmentioning

confidence: 99%

High genetic differentiation of Aegla longirostri (Crustacea, Decapoda, Anomura) populations in southern Brazil revealed by multi-loci microsatellite analysis

Bartholomei-Santos¹,

Roratto²,

Santos³

2011

Genet. Mol. Res.

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ABSTRACT. Species with a broad distribution rarely have the same genetic make-up throughout their entire range. In some cases, they may constitute a cryptic complex consisting of a few species, each with a narrow distribution, instead of a single-, widely distributed species. These differences can have profound impacts for biodiversity conservation planning. The genetic differentiation of four populations of Aegla longirostri, a freshwater crab found in two geographically isolated basins in Rio Grande do Sul State, Brazil, was investigated by analyzing pentanucleotide multi-loci microsatellites in a heteroduplex assay. Although no morphological differences were evident, we found significant genetic differentiation among the four populations, based on F ST values and clustering analysis. This high level of differentiation may be indicative of cryptic species in these populations. If this hypothesis is correct, then the species occurring in the Ibicuí-Mirim ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 4133-4146 (2011) M.L. River, at the southern limit of the Atlantic Rain Forest, would be under threat, considering its very restricted distribution.

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Human U1 small nuclear RNA genes: extensive conservation of flanking sequences suggests cycles of gene amplification and transposition.

Cited by 60 publications

References 66 publications

Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters

Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters

Structure and expression of a second sea urchin U1 RNA gene repeat

High genetic differentiation of Aegla longirostri (Crustacea, Decapoda, Anomura) populations in southern Brazil revealed by multi-loci microsatellite analysis

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