Human Umbilical Cord Blood Cells can be Induced to Express Markers for Neurons and Glia

Bicknese, Alma R.; Goodwin, H. S.; Quinn, Cheryl O.; Henderson, Verneake C D; Chien, Shin-Nan; Wall, Donna A.

doi:10.3727/096020197390022

Cited by 96 publications

(41 citation statements)

References 19 publications

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“…This fi nding is consistent with a number of recent studies showing that undifferentiated human cord blood-derived stem cells appear to have very limited or no ability to undergo transdifferentiation after intracranial transplantation [36,37], although these cells appeared to be able to express a variety of glial and neuronal markers in vitro [9,10,12,38]. The fact that the surviving MSCs possessed lamin immunoreactivity implies these cells maintained some properties characteristic of the donor of origin but failed to transdifferentiate.…”

Section: Cord Blood-derived Msc Differentiationsupporting

confidence: 91%

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells Do Not Differentiate Into Neural Cell Types or Integrate Into the Retina After Intravitreal Grafting in Neonatal Rats

Hill

Zwart²,

Tam³

et al. 2009

Stem Cells and Development

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This study investigated the ability of mesenchymal stem cells (MSCs) derived from full-term human umbilical cord blood to survive, integrate and differentiate after intravitreal grafting to the degenerating neonatal rat retina following intracranial optic tract lesion. MSCs survived for 1 week in the absence of immunosuppression. When host animals were treated with cyclosporin A and dexamethasone to suppress inflammatory and immune responses, donor cells survived for at least 3 weeks, and were able to spread and cover the entire vitreal surface of the host retina. However, MSCs did not significantly integrate into or migrate through the retina. They also maintained their human antigenicity, and no indication of neural differentiation was observed in retinas where retinal ganglion cells either underwent severe degeneration or were lost. These results have provided the first in vivo evidence that MSCs derived from human umbilical cord blood can survive for a significant period of time when the host rat response is suppressed even for a short period. These results, together with the observation of a lack of neuronal differentiation and integration of MSCs after intravitreal grafting, has raised an important question as to the potential use of MSCs for neural repair through the replacement of lost neurons in the mammalian retina and central nervous system.

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Section: Cord Blood-derived Msc Differentiationsupporting

confidence: 91%

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells Do Not Differentiate Into Neural Cell Types or Integrate Into the Retina After Intravitreal Grafting in Neonatal Rats

Hill

Zwart²,

Tam³

et al. 2009

Stem Cells and Development

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show abstract

“…The mononuclear hUCB cells that are cultured in specific conditioned media, including retinoic acid (RA) and nerve growth factor (NGF), expressed early neural markers such as musashi-1, nestin and neuronspecific class III beta-tubulin (TuJ1), mature neuronal markers such as neuronal nuclei (NeuN) and microtubuleassociated protein 2 (MAP-2) and astrocytic marker, glial fibrillary acidic protein (GFAP) [59]. Two different research groups Bicknese et al [60] and Buzanska et al [61] have also revealed that hUCB cells could differentiate into neural cell-like cells that express TuJ1, GFAP and galactosylceramide (GalC, oligodendrocytic marker). Especially, Buzanska et al [61] achieved neural stem cell-like cells using the CD34 − /CD45 − non-hematopoietic mononuclear cell fraction of hUCB.…”

Section: Transdifferentiation Into Neural Lineagesmentioning

confidence: 99%

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Park

Lee

Borlongan

et al. 2010

Stem Cell Rev and Rep

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Spinal cord injury (SCI) develops primary and secondary damage to neural tissue and this often results in permanent disability of the motor and sensory functions. However, there is currently no effective treatment except methylprednisolone, and the use of methylprednisolone has also been questioned due to its moderate efficacy and the drug's downside. Regenerative medicine has remarkably developed since the discovery of stem cells, and many studies have suggested the potential of cell-based therapies for neural injury. Especially, the therapeutic potential of human umbilical cord blood cells (hUCB cells) for intractable neurological disorders has been demonstrated using in vitro and vivo models. The hUCB cells are immune naïve and they are able to differentiate into other phenotypes, including the neural lineage. Their ability to produce several neurotropic factors and to modulate immune and inflammatory reactions has also been noted. Recent evidence has emerged suggesting alternative pathways of graft-mediated neural repair that involve neurotrophic effects. These effects are caused by the release of various growth factors that promote cell survival, angiogenesis and anti-inflammation, and this is all aside from a cell replacement mechanism. In this review, we present the recent findings on the stemness properties and the therapeutic potential of hUCB as a safe, feasible and effective cellular source for transplantation in SCI. These multifaceted protective and restorative effects from hUCB grafts may be interdependent and they act in harmony to promote therapeutic benefits for SCI. Nevertheless, clinical studies with hUCB are still rare because of the concerns about safety and efficiency. Among these concerns, the major histocompatibility in allogeneic transplantation is an important issue to be addressed in future clinical trials for treating SCI.

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“…In the last few years, there have been numerous studies demonstrating the plasticity of hUCB cells. These cells developed heterogeneous morphological characteristics atypical for hematopoietic-derived cells and express neural antigens in vitro (26)(27)(28), although it is not clear that their functional effects in vivo occur through this mechanism. Moreover, hUCB cells have been found to be effective in the treatment of animal models of stroke (29,30), traumatic brain (31) and spinal cord (32) injuries, and amyotropic lateral sclerosis (ALS) (33), probably providing protective trophic neuronal support by secretion of glialderived neurotrophic factor (GDNF) (34) or other growth factors.…”

Section: Introductionmentioning

confidence: 97%

Transplantation of Human Umbilical Cord Blood Cells Benefits an Animal Model of Sanfilippo Syndrome Type B

Garbuzova‐Davis

Willing

Desjarlais

et al. 2005

Stem Cells and Development

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Sanfilippo syndrome type B is caused by alpha-N-acetylglucosaminidase (Naglu) enzyme deficiency leading to an accumulation of undegraded heparan sulfate, a glycosaminoglycan (GAG). Cell therapy is a promising new treatment and human umbilical cord blood (hUCB) cell transplantation may be preferred for delivery of the missing enzyme. We investigated the ability of mononuclear hUCB cells administered into the lateral cerebral ventricle to ameliorate/prevent histopathological changes in mice modeling Sanfilippo syndrome type B. These are the first results supporting enzyme replacement by administered hUCB cells. In vivo, transplanted hUCB cells survived long-term (7 months), migrated into the parenchyma of the brain and peripheral organs, expressed neural antigens, and exhibited neuron and astrocyte-like morphology. Transplant benefits were also demonstrated by stable cytoarchitecture in the hippocampus and cerebellum, and by reduced GAGs in the livers of treated mutant mice. A hUCB cell transplant may be an effective therapeutic strategy for enzyme delivery in Sanfilippo syndrome type B.

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Human Umbilical Cord Blood Cells can be Induced to Express Markers for Neurons and Glia

Cited by 96 publications

References 19 publications

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells Do Not Differentiate Into Neural Cell Types or Integrate Into the Retina After Intravitreal Grafting in Neonatal Rats

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells Do Not Differentiate Into Neural Cell Types or Integrate Into the Retina After Intravitreal Grafting in Neonatal Rats

Transplantation of Umbilical Cord Blood Stem Cells for Treating Spinal Cord Injury

Transplantation of Human Umbilical Cord Blood Cells Benefits an Animal Model of Sanfilippo Syndrome Type B

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