Human Umbilical Cord Mesenchymal Stem Cell-induced Osterix, Bone Morphogenetic Protein-2, and Tartrate-resistant Acid Phosphatase Expression in Osteoporotic Mandibular Bone

Hendrijantini, Nike; Hartono, Cindy Karina; Daniati, Reni Puspa; Hong, Guang; Sitalaksmi, Ratri Maya; Kuntjoro, Mefina; Ari, Muhammad Dimas Aditya

doi:10.1055/s-0040-1715987

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…While TRAP activity is typically associated with myeloid lineage cells, including macrophages ( Fig. S8 ) and osteoclasts 29 , some mesenchymal lineage cells can also express TRAP 30 . Moreover, TBP-NPs increase the homing of NPs to areas of high TRAP activity such that TRAP - cells may also uptake NPs.…”

Section: Resultsmentioning

confidence: 99%

Development of a Nanoparticle-Based Tendon-Targeting Drug Delivery System to Pharmacologically Modulate Tendon Healing

Adjei-Sowah,

Chandrasiri,

Xiao

et al. 2023

Preprint

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Tendon regeneration following acute injury is marred by a fibrotic healing response that prevents complete functional recovery. Despite the high frequency of tendon injuries and the poor outcomes, including functional deficits and elevated risk of re-injury, there are currently no pharmacological therapies in clinical use to enhance the healing process. Several promising pharmacotherapies have been identified; however, systemic treatments lack tendon specificity, resulting in poor tendon biodistribution and perhaps explaining the largely limited beneficial effects of these treatments on the tendon healing process. To address this major unmet need, we leveraged our existing spatial transcriptomics dataset of the tendon healing process to identify an area of the healing tendon that is enriched for expression ofAcp5. Acp5encodes tartrate-resistant acid phosphatase (TRAP), and we demonstrate robust TRAP activity in the healing tendon. This unexpected finding allowed us to refine and apply our existing TRAP binding peptide (TBP) functionalized nanoparticle (NP) drug delivery system (DDS) to facilitate improved delivery of systemic treatments to the healing tendon. To demonstrate the translational potential of this drug delivery system, we delivered the S100a4 inhibitor, Niclosamide to the healing tendon. We have previously shown that genetic knockdown of S100a4 enhances tendon healing. While systemic delivery of Niclosamide did not affect the healing process, relative to controls, TBP-NP delivery of Niclosamide enhanced both functional and mechanical outcome measures. Collectively, these data identify a novel tendon-targeting drug delivery system and demonstrate the translational potential of this approach to enhance the tendon healing process.

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Section: Resultsmentioning

confidence: 99%

Development of a Nanoparticle-Based Tendon-Targeting Drug Delivery System to Pharmacologically Modulate Tendon Healing

Adjei-Sowah,

Chandrasiri,

Xiao

et al. 2023

Preprint

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“…This indicates that hUCMSCs can promote osteogenic differentiation and increase mineralization and bone formation in the osteoporotic bone. 8 In addition, hUCMSCs are useful for assisting the early-stage process of implant osseointegration in patients with osteoporosis. HUCMSCs have the potential to increase the proportions of direct bone-to-implant contact.…”

Section: Discussionmentioning

confidence: 99%

“…6 Dental implants have become an alternative for replacing one or more missing teeth or even the entire tooth in the edentulous jaw. 7,8 Several factors contribute to the successful integration of implants with bone, including the implant's surface, shape and topography, surgical technique, implant loading, and the quality and quantity of the implant recipient's bone. 9 Bone quality is the most critical factor in successful osseointegration and alveolar bone retention.…”

Section: Introductionmentioning

confidence: 99%

“…14 Other studies have also proved that hUCMSCs increased the number of osteoblasts, the expression of tumor growth factor-β1, runt-related transcription factor 2 (Runx2), alkaline phosphatase, collagen type I, osteocalcin, osterix (Osx), and bone morphogenetic protein 2 (BMP-2). 8 However, it is not yet known how extensive the effect of hUCMSCs induction is on the osseointegration process for osteoporosis patients using dental implants. Therefore, this study was conducted to evaluate the effect of hUCMSCs induction on the extent of osseointegration by the new bone formation area of dental implants in osteoporotic animal models.…”

Section: Introductionmentioning

confidence: 99%

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Bone formation and mineralization around the implant in osteoporotic animal models enhanced by mesenchymal stem cells

Hendrijantini,

Kuntjoro,

Agustono

et al. 2024

Dent. J.

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Background: Poor bone quality, sometimes caused by osteoporosis, can lead to dental implant failure. Human umbilical cord mesenchymal stem cells (hUCMSCs) can differentiate into osteoblasts and offer an alternative therapy for poor bone conditions. Purpose: This study aims to evaluate the effect of hUCMSCs induction on the extent of osseointegration by the new bone formation area of dental implants in osteoporotic animal models. Methods: The samples were divided into two groups, i.e., control and induced hUCMSCs groups at different times. An ovariectomy was performed to assess the osteoporosis condition. The control group was injected using gelatin, and the treatment group was administered hUCMSCs. Terminations were conducted at 2 and 4 weeks, respectively. The collected samples were then subjected to histological examination to analyze new bone formation and its proportion. In addition, alkaline phosphatase staining was also performed to evaluate the mineralization area. The data was analyzed using the one-way analysis of variance (ANOVA). Results: Both for new bone formation area as well as the proportion of new bone, it was observed that the best results were in the group with induction of hUCMSCs at 2 weeks. Alkaline phosphatase staining also confirmed that the highest mineralization was observed in the same group. Conclusion: hUCMSCs induced new bone formation in the implant placement in osteoporotic animal models.

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“…The fluorescence intensity of the cells was evaluated using FACScan (BD Biosciences, New Jersey, USA), and the data were then analyzed using CellQuest software version 6.0 (BD Biosciences, United Kingdom). 14…”

Section: Methodsmentioning

confidence: 99%

Osteoinductive and Osteogenic Capacity of Freeze-Dried Bovine Bone Compared to Deproteinized Bovine Bone Mineral Scaffold in Human Umbilical Cord Mesenchymal Stem Cell Culture: An In Vitro Study

et al. 2023

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Objective Freeze-dried bovine bone scaffold (FDBB) or decellularized FDBB (dc-FDBB) was developed as an ideal scaffold with osteoinductive properties. This research aims to compare the osteoinductive properties marked by the expression of runt-related transcription factor-2 (RUNX2) and Osterix (OSX) and the osteogenic capacity of these scaffolds imbued with human umbilical cord mesenchymal stem cells (hUCMSCs). Materials and Methods This study was performed in five experimental groups: a negative control group (C-) of hUCMSCs with a normal growth medium, a positive control group (C + ) of hUCMSCs with an osteogenic medium, experimental group 1 (E1) with an FDBB conditioned medium (CM), and experimental group 2 (E2) with a dc-FDBB-CM, and a third experimental group (E3) consisting of a DBBM-CM. Alizarin red staining was performed to qualitatively assess osteoinductive capacity. RUNX2 and OSX expression was quantified using real-time quantification polymerase chain reaction with two replications on day six (D6) and day 12 (D12) as fold changes. Results This experiment revealed that hUCMSCs were positively expressed by CD73, CD90, and CD105 but were not expressed by CD34. Alizarin red staining showed that E1 had the most calcium deposition on D6 and D12, followed by E3 and then E2 The RUNX2 and OSX expression was higher in E1 but this difference was not significant. The OSX expression in E1,E2,E3 was lower on D12 and C+ of OSX had the highest expression. There was a significant difference of fold change measured between all groups (p < 0.05), and there was no significant difference between any of the groups treated with OSX and RUNX2 on D6 and D12. Conclusion FDBB osteoinduction and osteogenic capacity were higher when compared with DBBM and dc-FDBB.

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Human Umbilical Cord Mesenchymal Stem Cell-induced Osterix, Bone Morphogenetic Protein-2, and Tartrate-resistant Acid Phosphatase Expression in Osteoporotic Mandibular Bone

Cited by 7 publications

References 22 publications

Development of a Nanoparticle-Based Tendon-Targeting Drug Delivery System to Pharmacologically Modulate Tendon Healing

Development of a Nanoparticle-Based Tendon-Targeting Drug Delivery System to Pharmacologically Modulate Tendon Healing

Bone formation and mineralization around the implant in osteoporotic animal models enhanced by mesenchymal stem cells

Osteoinductive and Osteogenic Capacity of Freeze-Dried Bovine Bone Compared to Deproteinized Bovine Bone Mineral Scaffold in Human Umbilical Cord Mesenchymal Stem Cell Culture: An In Vitro Study

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