Human umbilical vein endothelial cells increase ex vivo expansion of human CD34+ PBPC through IL-6 secretion

Li, N.; Eljaafari, Assia; Bensoussan, Danièle; Wang, Y.; Latger‐Cannard, Véronique; Serrurier, B.; Boura, Cédric; Kennel, Anne; Stoltz, Jean‐François; Feugier, Pierre

doi:10.1080/14653240600845062

Cited by 21 publications

(15 citation statements)

References 37 publications

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“…47,48 This finding is important because EPCs are known to express high levels of the IL-6 receptor (gp-80), and administration of IL-6 stimulates proliferation, migration, and tubule formation via activation of gp80/gp130 signaling pathways. 49,50 Furthermore, IL-8 can act as a proangiogenic cytokine with wound-healing properties, 51 and it also induces homing of EPCs. Patients with acute myocardial infarction show an increase in the levels of IL-8 and VEGF compared with patients with stable angina.…”

Section: Discussionmentioning

confidence: 99%

Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells

Bhatwadekar

Glenn

Curtis

et al. 2009

Invest. Ophthalmol. Vis. Sci.

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Purpose Bone marrow–derived endothelial progenitor cells (EPCs) contribute to vascular repair although it is uncertain how local endothelial cell apoptosis influences their reparative function. This study was conducted to determine how the presence of apoptotic bodies at sites of endothelial damage may influence participation of EPCs in retinal microvascular repair. Methods Microlesions of apoptotic cell death were created in monolayers of retinal microvascular endothelial cells (RMECs) by using the photodynamic drug verteporfin. The adhesion of early-EPCs to these lesions was studied before detachment of the apoptotic cells or after their removal from the wound site. Apoptotic bodies were fed to normal RMECs and mRNA levels for adhesion molecules were analyzed. Results Endothelial lesions where apoptotic bodies were left attached at the wound site showed a fivefold enhancement in EPC recruitment (P < 0.05) compared with lesions where the apoptotic cells had been removed. In intact RMEC monolayers exposed to apoptotic bodies, expression of ICAM, VCAM, and E-selectin was upregulated by 5- to 15-fold (P < 0.05– 0.001). EPCs showed a characteristic chemotactic response (P < 0.05) to conditioned medium obtained from apoptotic bodies, whereas analysis of the medium showed significantly increased levels of VEGF, IL-8, IL-6, and TNF-α when compared to control medium; SDF-1 remained unchanged. Conclusions The data indicate that apoptotic bodies derived from retinal capillary endothelium mediate release of proangiogenic cytokines and chemokines and induce adhesion molecule expression in a manner that facilitates EPC recruitment.

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Section: Discussionmentioning

confidence: 99%

Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells

Bhatwadekar

Glenn

Curtis

et al. 2009

Invest. Ophthalmol. Vis. Sci.

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“…Endothelial cells (ECs) were also shown to express inhibitory and stimulatory angiocrine factors that regulate the quiescence and proliferation of HSPCs and significantly augment the expansion of HSPCs[64, 76–78]. However, long-term maintenance of ECs requires stimulation with essential EC-growth factors and serum, that may affect their angiogenic properties and the HSPC expansion[79].…”

Section: Ex Vivo Expansion Of Transduced Hscsmentioning

confidence: 99%

Optimizing autologous cell grafts to improve stem cell gene therapy

Psatha

Karponi

Yannaki

2016

Experimental Hematology

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Over the past decade, stem cell gene therapy has achieved unprecedented curative outcomes for several genetic disorders. Despite the unequivocal success, clinical gene therapy still faces challenges. Genetically-engineered hematopoietic stem cells (HSCs) are particularly vulnerable to attenuation of their repopulating capacity once exposed to culture conditions, ultimately leading to low engraftment levels post-transplant. This becomes of particular importance when transduction rates are low or/and competitive transplant conditions are generated by a reduced intensity conditioning in the absence of a selective advantage of the transduced over the unmodified cells. These limitations could partially be overcome by introducing mega-doses of genetically-modified CD34+cells into conditioned patients or by transplanting HSCs with high engrafting and repopulating potential. In the present review, based on the lessons gained from the cord blood transplantation, we summarize the most promising approaches to date of increasing either the numbers of HSCs for transplantation or/and their engraftability, as a platform towards the optimization of engineered stem cell grafts.

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“…As such, it has been proposed that co-culture of HSCs with ECs is necessary to promote ex vivo expansion of HSCs. ECs from the brain, heart, and yolk sac have been shown to enhance expansion of human HSCs in vitro, which are capable of in vivo repopulation in mice and nonhuman primates [89–94]. …”

Section: Improving Homing Through Ex Vivo Hsc-endothelial Cell Co-culmentioning

confidence: 99%

Blood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment

2017

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Cells of the hematopoietic system undergo rapid turnover. Each day, humans require the production of about one hundred billion new blood cells for proper function. Hematopoietic stem cells (HSCs) are rare cells that reside in specialized niches and are required throughout life to produce specific progenitor cells that will replenish all blood lineages. There is, however, an incomplete understanding of the molecular and physical properties that regulate HSC migration, homing, engraftment, and maintenance in the niche. Endothelial cells (ECs) are intimately associated with HSCs throughout the life of the stem cell, from the specialized endothelial cells that give rise to HSCs, to the perivascular niche endothelial cells that regulate HSC homeostasis. Recent studies have dissected the unique molecular and physical properties of the endothelial cells in the HSC vascular niche and their role in HSC biology, which may be manipulated to enhance hematopoietic stem cell transplantation therapies.

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Human umbilical vein endothelial cells increase ex vivo expansion of human CD34+ PBPC through IL-6 secretion

Cited by 21 publications

References 37 publications

Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells

Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells

Optimizing autologous cell grafts to improve stem cell gene therapy

Blood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment

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