Nurse–patient interactions based on caring behaviors ensure better working conditions and better-quality healthcare. The aim of this quantitative study is to examine how nurses self-assess the frequency of applying caring behaviors in nurse–patient interactions and to identify the differences in the application frequency of caring behaviors in relation to work experience and education level. The respondents were Bachelor of Science (BSc) nurses and nurses with basic training (VET) employed in different clinical departments of the Clinical Hospital Center in Croatia. The survey used the “Caring Nurse–Patient Interactions Scale (Nurse Version)”. The respondents assessed the caring behaviors from the subscale “needs” as the most frequently applied (median (Me): 4.7; interquartile range (IQR): 4.4–4.9), while the least frequently applied were the procedures from the subscale “sensitivity” (Me: 3.8; IQR: 3.2–4.3). The VET nurses reported applying caring behaviors to the subscales “hope” (p < 0.001), “problem-solving” (p = 0.003), and “environment” (p = 0.021) more frequently than BSc nurses did. Compared with less experienced respondents, the respondents with more than 30 years of work experience applied the caring behaviors on the subscales “sensitivity” (p = 0.009), “expression of emotions” (p = 0.001), “problem-solving” (p = 0.008), and especially “humanism” and “spirituality” (p < 0.001) more frequently. The results indicate that respondents are more focused on applying skills or carrying out a task than on caring behaviors which is about demonstrating compassion, loving kindness, and relationships.