Humic acid capillary zone electrophoresis adsorption on capillary walls, separation in metal ion supplemented buffer and the fingerprints

Fetsch, David; Fetsch, M; Mendéz, Eladia Maria Peňa; Havel, Josef

doi:10.1002/elps.1150191419

Cited by 16 publications

(4 citation statements)

References 30 publications

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“…The formation of aggregates within solutions of humic acids was studied by capillary electrophoresis for the first time by Fetsch et al (1998aFetsch et al ( , 1998b. Humic acids (HAs) aggregation has also been studied in aqueous solution by high performance size exclusion chromatography (Peuravuori and Pihlaja 1997), light scattering (Manning et al 2000), vapor pressure osmometry (VPO) (Marinsky et al 1990), ultrafiltration (Aiken and Malcom (1987), conductometry and spectrophotometry in combination with factor analysis (Peña-Méndez et al 2004).…”

Section: Characterization Of Humic Substancesmentioning

confidence: 99%

Humic substances - compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine

Peña‐Méndez¹,

Havel²,

Patočka³

2005

J Appl Biomed

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306

173

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Humic substances as part of humus-soil organic matter-are compounds arising from the physical, chemical and microbiological transformation (humification) of biomolecules. They are important because they constitute the most ubiquitous source of non-living organic material that nature knows. Approximately 80% of the total carbon in terrestrial media and 60% of the carbon dissolved in aquatic media are made up of humic substances. Humic substances have important roles in soil fertility, and are considered to have primal relevance for the stabilization of soil aggregates. They can be divided into three components according to their solubility: humic acids, fulvic acids and humin. Humic acids are the most explored group of humic substances. Beyond their relevance for life these substances have industrial applications in the development of absorbents to be used at the sources of metal-poisoning. Being natural substances, their purification process is cheaper than the synthesis of any other sorbent and, moreover, due to their high operability, they absorb more than the absorbents used to date, such as active charcoals or clays. The specific properties of humic acid products enable their application in industry, agriculture, environmental and biomedicine.

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Section: Characterization Of Humic Substancesmentioning

confidence: 99%

Humic substances - compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine

Peña‐Méndez¹,

Havel²,

Patočka³

2005

J Appl Biomed

Self Cite

306

173

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“…Special attention was paid to CZE as it combines the unique possibility to separate and detect NOM in aqueous solution within wide pH-and ionic strengthranges that allow mimicking environmental conditions. Characterizations were performed mainly using uncoated silica capillaries except in few cases where the authors aimed to minimize the electroosmotic flow (EOF) [29] or HS-sorption onto the capillary wall at low pH [30,31]. Among all separation electrolytes mentioned in the literature, the most frequently used are based on acetate [29,32], borate [29,32,33], phosphate [29,[32][33][34] or phosphate-borate [33,35] buffer systems.…”

Section: Introductionmentioning

confidence: 99%

Contribution of capillary electrophoresis to an integrated vision of humic substances size and charge characterizations

d’Orlyé

Reiller

2012

Journal of Colloid and Interface Science

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The physicochemical properties of three different humic substances (HS) are probed using capillary zone electrophoresis in alkaline carbonate buffers, pH 10. Special attention is drawn to the impact of the electrolyte ionic strength and counter-ion nature, chosen within the alkali-metal series, on HS electrophoretic mobility. Taylor-Aris dispersion analysis provides insights into the hydrodynamic radius (R(H)) distributions of HS. The smallest characterized entities are of nanometric dimensions, showing neither ionic strength- nor alkali-metal-induced aggregation. These results are compared with the entities evidenced in dynamic light scattering measurements, the size of which is two order of magnitude higher, ca. 100 nm. The extended Onsager model provides a reasonable description of measured electrophoretic mobilities in the ionic strength range 1-50 mM, thus allowing the estimation of limiting mobilities and ionic charge numbers for the different HS samples. An unexpected HS electrophoretic mobility increase (in absolute value) is observed in the order Li(+)

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“…Low pH has been found to promote formation of dark QDs (nonfluorescent), which may have caused the detection problems encountered at pH 5 in this study. A pH 10 buffer was selected in the present study to minimize HS adsorption on the capillary wall …”

Section: Resultsmentioning

confidence: 99%

“…A pH 10 buffer was selected in the present study to minimize HS adsorption on the capillary wall. 55 In the first setup of CE experiments, increasing amounts of SRHA or SRFA were mixed with the running buffer, while injecting a constant amount of QDs. This setup was designed to investigate the instantaneous effect of HS on the fluorescence intensities and electrophoretic mobilities of QDs.…”

Section: ' Results and Discussionmentioning

confidence: 99%

Study on the Effects of Humic and Fulvic Acids on Quantum Dot Nanoparticles Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Celiz

Colón

Watson

et al. 2011

Environ. Sci. Technol.

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The increasing production and use of quantum dot (QD) nanoparticles have caused concerns on the possibility of contaminating the aquatic and terrestrial ecosystems with wastes that may contain QDs. Therefore, studies on the behavior of QDs upon interaction with components of the natural environment have become of interest. This study investigated the fluorescence and electrophoretic mobility of carboxylic or amine polyethylene glycol (PEG)-functionalized CdSe/ZnS QDs in the presence of two aquatic humic substances (HS), Suwannee River humic and fulvic acids, using capillary electrophoresis with laser-induced fluorescence detection. Results showed initial enhancement in fluorescence of QDs at the onset of the interaction with HS, followed by fluorescence quenching at longer exposure with HS (>30 min). It was also observed that the electrophoretic mobility of QDs increases with increasing concentration of HS, suggesting an increase in the ratio in charge to hydrodynamic size of the nanoparticles. To determine if the QDs degraded upon interaction with HS, the QD-HS mixtures were dialyzed to separate free Cd2+ from intact QDs, followed by analysis of the solutions using inductively coupled plasma-mass spectrometry. Results suggested that degradation of QDs in the presence of HS did not occur within the period of incubation.

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Humic acid capillary zone electrophoresis adsorption on capillary walls, separation in metal ion supplemented buffer and the fingerprints

Cited by 16 publications

References 30 publications

Humic substances - compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine

Humic substances - compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine

Contribution of capillary electrophoresis to an integrated vision of humic substances size and charge characterizations

Study on the Effects of Humic and Fulvic Acids on Quantum Dot Nanoparticles Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection

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