Humidity response depends on the small soluble protein Obp59a in Drosophila

Sun, Jennifer S; Larter, Nikki K; Chahda, Juan Sebastian; Rioux, Douglas; Gumaste, Ankita; Carlson, John R.

doi:10.7554/elife.39249

Cited by 57 publications

(56 citation statements)

References 44 publications

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“…One functional study in D. melanogaster found robust OSN responses even after the sole OBP from the corresponding sensillum had been knocked-out (Larter et al 2016). Another study identified a role for an OBP in humidity sensing (Sun et al 2018). It, therefore, remains to be seen how OBPs or other accessory proteins might contribute to the evolution of olfactory behavior (but see Matsuo et al 2007 for a clear example of an OBP driving the evolution of taste responses).…”

Section: Changes In Osn Tuning Via Accessory Protein Evolutionmentioning

confidence: 99%

Evolution of olfactory circuits in insects

Zhao

McBride

2020

J Comp Physiol A

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Recent years have seen an explosion of interest in the evolution of neural circuits. Comparison of animals from different families, orders, and phyla reveals fascinating variation in brain morphology, circuit structure, and neural cell types. However, it can be difficult to connect the complex changes that occur across long evolutionary distances to behavior. Luckily, these changes accumulate through processes that should also be observable in recent time, making more tractable comparisons of closely related species relevant and complementary. Here, we review several decades of research on the evolution of insect olfactory circuits across short evolutionary time scales. We describe two well-studied systems, Drosophila sechellia flies and Heliothis moths, in detailed case studies. We then move through key types of circuit evolution, cataloging examples from other insects and looking for general patterns. The literature is dominated by changes in sensory neuron number and tuning at the periphery-often enhancing neural response to odorants with new ecological or social relevance. However, changes in the way olfactory information is processed by central circuits is clearly important in a few cases, and we suspect the development of genetic tools in non-model species will reveal a broad role for central circuit evolution. Moving forward, such tools should also be used to rigorously test causal links between brain evolution and behavior.

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Section: Changes In Osn Tuning Via Accessory Protein Evolutionmentioning

confidence: 99%

Evolution of olfactory circuits in insects

Zhao

McBride

2020

J Comp Physiol A

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“…Many of the most highly expressed genes in the antenna are those encoding Odorant Binding Proteins (OBPs), which are transcribed in nonneuronal cells, often at >10-fold higher levels than olfactory receptor genes, as determined by bulk RNA-seq 31,34 . Two of these, Obp59a and Obp84a are present in sensilla housing Ir neurons 37,38 , but neither of these genes display significant OSs in any neuron population ( Figure 1G). Similarly, markers for other support cell types, such as nompA (thecogen cell) 37,39 , and the glial marker repo are not significantly occupied ( Figure 1G).…”

Section: Results Targeted Damid Of Osn Populationsmentioning

confidence: 99%

Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Arguello

Abuin

Armida

et al. 2020

Preprint

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AbstractDetermining the molecular properties of neurons is essential to understand their development, function and evolution. We used Targeted DamID (TaDa) to characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic Receptor (IR)-expressing sensory neurons in the Drosophila antenna. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not linked to substantial differences in chromatin accessibility, but rather to distinct transcription factor profiles. Heterogeneously-expressed genes across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.

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“…Nevertheless, there are some OBPs that are conserved and have orthology relationships with counterparts from other Dipterans. For example, HillOBP1 and 2 are homolog to OBP83b found in B. dorsalis, HillOBP11 is a homolog to Lush found in D. melanogaster, and HillOBP9 is a homolog to DmelOBP59a found in D. melanogaster (Pikielny et al, 1994;Galindo and Smith, 2001;Wu et al, 2015;Sun et al, 2018). Both OBP83b and Lush have been reported to play roles in sensing semiochemical attractants and the pheromone 11-cis vaccenyl acetate (Xu et al, 2005;Wu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Profiling of Chemosensory Genes in Hermetia illucens via a Transcriptomic Analysis

Zeng

et al. 2020

Front. Physiol.

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The black soldier fly, Hermetia illucens, is a cosmopolitan insect of the family Stratiomyidae (Diptera). Chemosensory genes encode proteins involved directly in the detection of odorants. In this study, we sequenced the antennal transcriptome of H. illucens adults to identify chemosensory genes. Putative unigenes encoding 27 odorant binding proteins (OBPs), five chemosensory proteins (CSPs), 70 odorant receptors (ORs), 25 ionotropic receptors (IRs), 10 gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) were identified. Tissue-specific expression profiles of the identified OBPs, CSPs and SNMPs were investigated using RT-PCR. Eight OBPs (HillOBP1-2, 9, 11-14, and 17), one CSP (HillCSP5) and one SNMP (HillSNMP1) were predominantly expressed in antennae. Further real-time quantitative PCR analyses revealed that the antennae-enriched unigenes also exhibited significant differences in expression between males and females. Among the sex-biased unigenes, six ORs showed female-biased expression, suggesting that these genes might participate in female-specific behaviors such as oviposition site searching. Sixteen ORs and two OBPs showed male-biased expression, indicating that they may play key roles in the detection of female sex pheromones. Our study is the first attempt to delineate the molecular basis of chemoreception in H. illucens. Our data provide useful information for comparative studies on the differentiation and evolution of Dipteran chemosensory gene families.

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Humidity response depends on the small soluble protein Obp59a in Drosophila

Cited by 57 publications

References 44 publications

Evolution of olfactory circuits in insects

Evolution of olfactory circuits in insects

Targeted molecular profiling of rare cell populations identifies olfactory sensory neuron fate and wiring determinants

Identification and Expression Profiling of Chemosensory Genes in Hermetia illucens via a Transcriptomic Analysis

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