Humification in extracellular polymeric substances (EPS) dominates methane release and EPS reconstruction during the sludge stabilization of high-solid anaerobic digestion

Tang, Yanfei; Dai, Xiaohu; Dong, Bin; Guo, Yiqun; Dai, Lingling

doi:10.1016/j.watres.2020.115686

Cited by 139 publications

(15 citation statements)

References 58 publications

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“…Figure 1 represents the synthesis pathway of microbial EPS. The bacterial biosynthetic pathways include (a) substrate digestion [ 35 ], (b) main metabolite pathway [ 36 ] and (c) polysaccharides synthesis [ 37 , 38 ]. Depending on the form of the substrate, either passive or active transport system, it may be absorbed by the cell, and catabolized through intracellular phosphorylation, or perhaps a direct oxidative periplasm could pass and oxidize it [ 39 ].…”

Section: Biosynthetic Pathwaysmentioning

confidence: 99%

Recent Advancements in Microbial Polysaccharides: Synthesis and Applications

et al. 2021

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Polysaccharide materials are widely applied in different applications including food, food packaging, drug delivery, tissue engineering, wound dressing, wastewater treatment, and bioremediation sectors. They were used in these domains due to their efficient, cost-effective, non-toxicity, biocompatibility, and biodegradability. As is known, polysaccharides can be synthesized by different simple, facile, and effective methods. Of these polysaccharides are cellulose, Arabic gum, sodium alginate, chitosan, chitin, curdlan, dextran, pectin, xanthan, pullulan, and so on. In this current article review, we focused on discussing the synthesis and potential applications of microbial polysaccharides. The biosynthesis of polysaccharides from microbial sources has been considered. Moreover, the utilization of molecular biology tools to modify the structure of polysaccharides has been covered. Such polysaccharides provide potential characteristics to transfer toxic compounds and decrease their resilience to the soil. Genetically modified microorganisms not only improve yield of polysaccharides, but also allow economically efficient production. With the rapid advancement of science and medicine, biosynthesis of polysaccharides research has become increasingly important. Synthetic biology approaches can play a critical role in developing polysaccharides in simple and facile ways. In addition, potential applications of microbial polysaccharides in different fields with a particular focus on food applications have been assessed.

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Section: Biosynthetic Pathwaysmentioning

confidence: 99%

Recent Advancements in Microbial Polysaccharides: Synthesis and Applications

et al. 2021

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“…They also proposed that the aromatic repolymerisation of humics regulated the phytotoxicity of digestate via reducing excessive salinity. Based on these findings, they further investigated the effect of humification on extracellular polymeric substances (EPS) in the HS-AD of SS (Tang et al, 2020), and found that the hydrolysis and decomposition of extracellular protein generated changes in the highly cross-linked structures of humics in EPS, resulting in the exposure of humic aromatic groups and binding sites. Based on analysis of the electron exchange capacity and the metabolic activity of methanogenesis, they proposed that structural changes in EPS proteins promoted the catabolism and anabolism of anaerobic microorganisms, and that the products of this metabolism, such as humic groups and active protein derivatives, were beneficial to EPS reconstruction in the HS-AD of SS.…”

Section: Organic Humification In the Hs-ad Of Ssmentioning

confidence: 99%

High-solid anaerobic digestion of sewage sludge: achievements and perspectives

Gong

Dai

2020

Front. Environ. Sci. Eng.

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High-solid anaerobic digestion (HS-AD) has been applied extensively during the last few decades for treating various organic wastes, such as agricultural wastes, organic fractions of municipal solid wastes, and kitchen wastes. However, the application of HS-AD to the processing of sewage sludge (SS) remains limited, which is largely attributable to its poor process stability and performance. Extensive research has been conducted to attempt to surmount these limitations. In this review, the main factors affecting process stability and performance in the HS-AD of SS are comprehensively reviewed, and the improved methods in current use, such as HS sludge pre-treatment and anaerobic co-digestion with other organic wastes, are summarised. Besides, this paper also discusses the characteristics of substance transformation in the HS-AD of SS with and without thermal pre-treatment. Research has shown that the HS effect is due to the presence of high concentrations of substances that may inhibit the function of anaerobic microorganisms, and that it also results in poor mass transfer, a low diffusion coefficient, and high viscosity. Finally, knowledge gaps in the current research on HS-AD of SS are identified. Based on these, it proposes that future efforts should be devoted to standardising the definition of HS sludge, revealing the law of migration and transformation of pollutants, describing the metabolic pathways by which specific substances are degraded, and establishing accurate mathematical models. Moreover, developing green sludge dewatering agents, obtaining high value-added products, and revealing effects of the above two on HS-AD of SS can also be considered in future.

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“…For example, Tang et al (2020) found the original HA in extracellular polymeric substances could be degraded and modified, and HA with abundant aromatic sites may also bridge protein condensation to regenerate HA during sewage sludge AD process (Tang et al, 2020). Moreover, during humification, precursors from different sources (feeding materials in the AD process) would enable the formation of HA with different molecular compositions, structures, and functionalities due to the different elemental compositions and properties of raw materials (Sale et al, 2015;He et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, current knowledge about HA formation mechanisms is largely derived from research on composting, which is primarily led by a group of aerobic microorganisms (Gao et al, 2019;He et al, 2015). Although the phenomenon of HA formation has been observed in the anaerobic digestion (Tang et al, 2018;Tang et al, 2020), the evolutionary dynamics of HA in the AD process, which is dominated by anaerobic microorganisms,…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of genuine humic acid evolution and its dynamic interaction with methane production in anaerobic digestion processes

Wang

Muhmood

Lyu

et al. 2021

Chemical Engineering Journal

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Humic acid (HA), a byproduct formed during the biological conversion of organic matter into biogas in the anaerobic digestion (AD) process, contains complex structures and redox functions. However, the evolution mechanism of HA and its interaction with CH4 production during the AD process have not been fully explored, particularly with respect to various substrates and temperature conditions. In this study, we investigated the evolutionary dynamics of the structure and function of genuine HA that naturally formed in the AD processes of chicken manure and corn stover under mesophilic (37 °C) and thermophilic (55 °C) conditions. The results demonstrated that the HA evolution mechanisms in AD of chicken manure and corn stover have different pathways. The AD of core stover showed higher degree of aromaticity (41.2-66.7% and 45.3-68.4% for mesophilic and thermophilic respectively) and humification index (1.5-4.2 and 2.8-4.5 for mesophilic and thermophilic respectively) than those (28.3-45.3% and 30.2-54.5% of aromaticity and 0.6-1.2 and 1.3-3.7 of humification index) in AD of chicken manure. The

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Humification in extracellular polymeric substances (EPS) dominates methane release and EPS reconstruction during the sludge stabilization of high-solid anaerobic digestion

Cited by 139 publications

References 58 publications

Recent Advancements in Microbial Polysaccharides: Synthesis and Applications

Recent Advancements in Microbial Polysaccharides: Synthesis and Applications

High-solid anaerobic digestion of sewage sludge: achievements and perspectives

Mechanisms of genuine humic acid evolution and its dynamic interaction with methane production in anaerobic digestion processes

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