With the rise of prejudice and discrimination against ethnic and immigrant minorities, strategies to reduce prejudice and discrimination, and to counteract the impact of intolerant, anti-egalitarian ideologies, are needed. Here we focused on cultural humility, i.e., the ability to have a humble and other-oriented approach to others’ cultural backgrounds, resulting from self-examination and critical thinking about structural privileges and inequalities. In this research we proposed that cultural humility might attenuate the effects of intolerant, anti-egalitarian ideologies such as social dominance orientation (SDO) and right-wing authoritarianism (RWA) on negative intergroup attitudes and perceptions. In a correlational study conducted in Italy, we found that cultural humility moderated the associations between SDO and prejudice toward immigrants, as well as between SDO and perceptions of threat posed by immigrants. Specifically, the associations of SDO with prejudice and threat were lower among respondents with high cultural humility compared to respondents with low cultural humility. Conversely, cultural humility did not moderate the effects of RWA on prejudice and threat. Findings are discussed considering the motivations underlying prejudice of high-SDO and high-RWA individuals, and proposing cultural humility training to foster positive intergroup relations.