Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE)

Tulinská, Jana; Adel‐Patient, Karine; Bernard, Hervé; Líšková, Aurélia; Kuricová, Miroslava; Ilavská, Silvia; Horváthová, Mira; Kebis, Anton; Rollerová, Eva; Babincová, Júlia; Aláčová, Radka; Wal, Jean‐Michel; Schmidt, Kerstin; Schmidtke, Jörg; Schmidt, Peter L.; Kohl, Christian; Wilhelm, Ralf; Schiemann, Joachim; Steinberg, Pablo

doi:10.1007/s00204-018-2230-z

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…Many studies now indicate that exposure to GMOs affects health in lab animals, livestock, and aquatic animals (reviewed in [9,10,11,12]. Animal GMO-feeding studies have identified health concerns affecting multiple organ systems including cardiovascular [13,14], digestive [15,16,17], exocrine [18,19], hepatorenal [9,13,20,21,22,23,24,25], immune [26,27,28], and reproductive systems [15,23,27,29,30,31]. Within these systems, traits that differed between GMO-exposed and control groups include cell proliferation [9,32], histology [15,16,18,23,30,31], hematology [14,20,25,28], metabolism [19,21,23,27,31,33,34], milk production [27], and lifespan and mortality [9,29].…”

Section: Introductionmentioning

confidence: 99%

“…Animal GMO-feeding studies have identified health concerns affecting multiple organ systems including cardiovascular [13,14], digestive [15,16,17], exocrine [18,19], hepatorenal [9,13,20,21,22,23,24,25], immune [26,27,28], and reproductive systems [15,23,27,29,30,31]. Within these systems, traits that differed between GMO-exposed and control groups include cell proliferation [9,32], histology [15,16,18,23,30,31], hematology [14,20,25,28], metabolism [19,21,23,27,31,33,34], milk production [27], and lifespan and mortality [9,29]. Some of these studies [15,17,23,31] used feed containing both HT and Bt ingredients, while others exposed animals to diets containing either HT [9,13,18,19,21,22,25,27,30,33,34,35] or Bt [14,…”

Section: Introductionmentioning

confidence: 99%

“…Within these systems, traits that differed between GMO-exposed and control groups include cell proliferation [9,32], histology [15,16,18,23,30,31], hematology [14,20,25,28], metabolism [19,21,23,27,31,33,34], milk production [27], and lifespan and mortality [9,29]. Some of these studies [15,17,23,31] used feed containing both HT and Bt ingredients, while others exposed animals to diets containing either HT [9,13,18,19,21,22,25,27,30,33,34,35] or Bt [14,16,20,24,25,26,28,29,32] ingredients. However, most of these studies do not address the mechanism that does cause harm from GMO exposure.…”

Section: Introductionmentioning

confidence: 99%

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Roundup®, but Not Roundup-Ready® Corn, Increases Mortality of Drosophila melanogaster

Talyn

Lemon

Badoella

et al. 2019

Toxics

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Genetically modified foods have become pervasive in diets of people living in the US. By far the most common genetically modified foods either tolerate herbicide application (HT) or produce endogenous insecticide (Bt). To determine whether these toxicological effects result from genetic modification per se, or from the increase in herbicide or insecticide residues present on the food, we exposed fruit flies, Drosophila melanogaster, to food containing HT corn that had been sprayed with the glyphosate-based herbicide Roundup®, HT corn that had not been sprayed with Roundup®, or Roundup® in a variety of known glyphosate concentrations and formulations. While neither lifespan nor reproductive behaviors were affected by HT corn, addition of Roundup® increased mortality with an LC50 of 7.1 g/L for males and 11.4 g/L for females after 2 days of exposure. Given the many genetic tools available, Drosophila are an excellent model system for future studies about genetic and biochemical mechanisms of glyphosate toxicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Roundup®, but Not Roundup-Ready® Corn, Increases Mortality of Drosophila melanogaster

Talyn

Lemon

Badoella

et al. 2019

Toxics

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“…More specifically, for assessing adjuvanticity, most studies focused on Bacillus thuringiensis (Bt)‐maize Cry1 proteins or including the whole food matrix (Vázquez‐Padrón et al, 1999 ; Vázquez et al 1999 ; Guimaraes et al, 2008 ; Reiner et al, 2014 ; Andreassen et al, 2016 ; Tulinska et al, 2018 ). However, other studies addressed the potential adjuvant effect of the bean alpha‐amylase inhibitor in GM peas (Prescott et al, 2005 ; Lee et al, 2013 ).…”

Section: Assessmentmentioning

confidence: 99%

Scientific Opinion on development needs for the allergenicity and protein safety assessment of food and feed products derived from biotechnology

Mullins¹,

Bresson²,

Dalmay³

et al. 2022

EFS2

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This Scientific Opinion addresses the formulation of specific development needs, including research requirements for allergenicity assessment and protein safety, in general, which is urgently needed in a world that demands more sustainable food systems. Current allergenicity risk assessment strategies are based on the principles and guidelines of the Codex Alimentarius for the safety assessment of foods derived from 'modern' biotechnology initially published in 2003. The core approach for the safety assessment is based on a 'weight-of-evidence' approach because no single piece of information or experimental method provides sufficient evidence to predict allergenicity. Although the Codex Alimentarius and EFSA guidance documents successfully addressed allergenicity assessments of single/ stacked event GM applications, experience gained and new developments in the field call for a modernisation of some key elements of the risk assessment. These should include the consideration of clinical relevance, route of exposure and potential threshold values of food allergens, the update of in silico tools used with more targeted databases and better integration and standardisation of test materials and in vitro/in vivo protocols. Furthermore, more complex future products will likely challenge the overall practical implementation of current guidelines, which were mainly targeted to assess a few newly expressed proteins. Therefore, it is timely to review and clarify the main purpose of the allergenicity risk assessment and the vital role it plays in protecting consumers' health. A roadmap to (re)define the allergenicity safety objectives and risk assessment needs will be required to inform a series of key questions for risk assessors and risk managers such as 'what is the purpose of the allergenicity risk assessment?' or 'what level of confidence is necessary for the predictions?'.

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“…Ten mice in each group were selected randomly to calculate the volume and weight of the tumor, immune organ (thymus and spleen) index and cellular immunity. The spleen and thymus were collected from each mouse, and the growth index for each thymus or spleen was recorded [16].…”

Section: Tumor Incidence and Immune Organ Indexmentioning

confidence: 99%

Antigens from H22 cell mutation induced by lentinan are correlated with HCC immunoprophylaxis

Yang¹,

Li²,

Wen³

et al. 2020

Preprint

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Background Lentinan can inhibit the proliferation of many kinds of tumor cells, and some studies have shown that lentinan can inhibit the growth of tumor tissue by directly killing tumor cells. The purpose of this study was to investigate the immunoprophylaxis effect and mechanism of tumor specific antigen induced by lentinan on liver cancer in mice.Methods The immunogen was prepared which was induced by lentinan. And two different mouse H22 liver cancer models (ascites tumor and solid tumor)were established after immunogen immunized. The tumor growth and immune organ index of mice in each group were measured. In order to analyze the immune mechanism, the activity of CTL cells, activation of ADCC pathway ,T lymphocyte subsets, expression of CD4 + T,CD8 + T cells and expression of DC, and surface molecular markers CD80 and CD86 in immunized mice were analyzed. The expression of cytokines such as IFN-γ and TNF-α in serum and cell supernatant of mice in each group was tested too. Finally, the immunogen was analyzed by Liquid Chromatography-Mass Spectrometry (LC-MS), and the functional proteins were screened for further analysis.Results The liver, spleen and thymus of the mice immunized with immunogen were protected to a certain extent. In immunized mice, the proportion of CD4 + T cells increased, the proportion of CD8 + T cells decreased, the ratio of CD4 + T/CD8 + T increased, and the killing activity of T lymphocytes increased significantly. The expression of CD80 in mature DC cells of immunized mice increased and cytokines increased significantly. The results of protein mass spectrometry analysis of immunogen showed that immunogen produced protein properties that inhibit cancer.Conclusion The H22 whole-cell cleavage vaccine induced by lentinan is effective in the immunity of mouse hepatoma H22 and has development value in the future.

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Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE)

Cited by 15 publications

References 26 publications

Roundup®, but Not Roundup-Ready® Corn, Increases Mortality of Drosophila melanogaster

Roundup®, but Not Roundup-Ready® Corn, Increases Mortality of Drosophila melanogaster

Scientific Opinion on development needs for the allergenicity and protein safety assessment of food and feed products derived from biotechnology

Antigens from H22 cell mutation induced by lentinan are correlated with HCC immunoprophylaxis

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