Humoral and Cellular Immune Responses to Mycoplasmas

Fernald, Gerald W.

doi:10.1016/b978-0-12-078402-8.50018-8

Cited by 23 publications

(22 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1,9,11,16,17,19 Mh organisms, after colonizing the respiratory tract, stimulate alveolar macrophages to secrete cytokines that induce a persistent, immune and inflammatory response. 1 These cytokines, nonspecific mitogenic activity of Mh on lymphocytes, 19 or a number of immunogenic mycoplasmal proteins 28 have been incriminated in the intense lymphocyte accumulation around bronchi and bronchioles, infiltration of inflammatory cells into the airways, and lymphoid hyperplasia characteristic of Mh-infected pigs.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that this contributes to the formation of the lymphoid accumulations seen in the lungs of mice, rats, hamsters, cattle, and pigs as a result of mycoplasma infections. 6,9,13,25 Several mycoplasmal products and mycoplasma membrane proteins, which are immunogenic during Mh infection, have been described so far as being responsible for the massive lymphoid hyperplasia around airways. 11,17,19,22,26,29 Histologic lesions in the chronic severe stages of PEN are characterized by lymphoid hyperplasia of the bronchus-associated lymphoid tissue (BALT) causing obliteration of the lumens of bronchioles and atelectasis of surrounding alveoli.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Morphologic and Immunohistochemical Study of the Bronchus-associated Lymphoid Tissue of Pigs Naturally Infected withMycoplasma hyopneumoniae

et al. 2003

View full text Add to dashboard Cite

Abstract. Porcine enzootic pneumonia (PEN), caused by Mycoplasma hyopneumoniae (Mh), has been described in pigs in all geographic areas. The disease is characterized by high morbidity and low mortality rates in intensive swine production systems. A morphologic and immunohistochemical study was done to determine the cellular populations present in lung parenchyma of infected pigs, with special attention to the bronchusassociated lymphoid tissue (BALT). Polyclonal and monoclonal antibodies were used for the detection of antigens of Mh, T lymphocytes (CD3 ϩ , CD4 ϩ , and CD8 ϩ ), IgG ϩ or IgA ϩ lymphocytes, and cells containing lysozyme, S-100 protein, major histocompatibility complex class II antigen or myeloid-histiocyte antigen. Findings in lung tissues associated with Mh infection were catarrhal bronchointerstitial pneumonia, with infiltration of inflammatory cells in the lamina propria of bronchi and bronchioles and alveolar septa. Hyperplasia of mononuclear cells in the BALT areas was the most significant histologic change. The BALT showed a high morphologic and cellular organization. Macrophages and B lymphocytes were the main cellular components of germinal centers. T lymphocytes were primarily located in perifollicular areas of the BALT, lamina propria and within the airway epithelium, and plasma cells containing IgG or IgA at the periphery of the BALT, in the lamina propria of bronchi and bronchioles, in alveolar septa, and around bronchial submucosal glands. The hyperplastic BALT in PEN cases consisted of macrophages, dendritic cells, T and B lymphocytes, and IgG ϩ and IgA ϩ plasma cells. CD4 ϩ cells predominated over CD8 ϩ cells. Local humoral immunity appears to play an important role in the infection.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Morphologic and Immunohistochemical Study of the Bronchus-associated Lymphoid Tissue of Pigs Naturally Infected withMycoplasma hyopneumoniae

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The pathogenesis of Mycoplasmapneumoniae diseases of the respiratory tract is, to a considerable extent, determined by immune reactions of the host [5,7]. These adverse events contribute to the severity of an atypical pneumonia and can be based on an exacerbation of host's cellular response mechanisms and/or the induction of autoantibodies.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the cellular response of spleen cells in BALB/c mice inoculated with Mycoplasma pneumoniae or the P1 protein

Pietsch

Jacobs

1993

Med Microbiol Immunol

View full text Add to dashboard Cite

BALB/c mice were intranasally infected or intraperitoneally inoculated with Mycoplasma pneumoniae whole cells or were immunized with the isolated adhesin (P1 protein). Spleen cells were isolated and tested in vitro for proliferation activity after stimulation with the P1 protein and sonicated M. pneumoniae whole antigen preparations. In frequency analysis experiments the P1 protein-specific proliferative response of spleen lymphocytes increased from 1/11494 in mice immunized once to 1/3246 in eightfold-inoculated mice, demonstrating that the P1 protein is a prominent immunogen of M. pneumoniae cells. Depletion experiments showed that T and B cells are activated in a 2:1 relation. Fluorescence-activated cells sorting analysis revealed a shift of the CD4/CD8 ratio from 2:1 in control mice up to 3:1 in M. pneumoniae-, and to 3.4:1 in P1 protein-immunized mice, as well as an increase in interleukin 2 receptor-bearing cells and macrophage cell populations. The results indicate that this animal model is appropriate to study host-M. pneumoniae interactions and vaccination schedules.

show abstract

“…The intended use of this protein for local immunoprophylaxis against M . pneumoniae infections (Fernald, 1979;Razin, 1985) may therefore not be as promising as previously expected.…”

Section: Discussionmentioning

confidence: 75%

Immunological Reaction of Guinea-pigs Following Intranasal Mycoplasma pneumoniae Infection and Immunization with the 168 kDa Adherence Protein

Jacobs

Drews²,

Stuhlert³

et al. 1988

Microbiology

View full text Add to dashboard Cite

Humoral responses to Mycoplasma pneumoniae proteins, especially the 168 kDa protein, were demonstrated by Western blotting in sera and bronchial washings of all groups of infected or immunized guinea-pigs. However, infection was not prevented by these local and systemic antibodies. Hilar lymphocytes of infected and immunized guinea-pigs were stimulated in vitro by sonicated M. pneumoniae antigen and by the 168 kDa protein. Stimulation was significantly lower in animals which had been infected twice or had been preimmunized and challenged by infection. Histologically the most severe lesions were seen in the twice-infected group followed by the preimmunized group which was subsequently infected.

show abstract

Humoral and Cellular Immune Responses to Mycoplasmas

Cited by 23 publications

References 86 publications

A Morphologic and Immunohistochemical Study of the Bronchus-associated Lymphoid Tissue of Pigs Naturally Infected withMycoplasma hyopneumoniae

A Morphologic and Immunohistochemical Study of the Bronchus-associated Lymphoid Tissue of Pigs Naturally Infected withMycoplasma hyopneumoniae

Characterization of the cellular response of spleen cells in BALB/c mice inoculated with Mycoplasma pneumoniae or the P1 protein

Immunological Reaction of Guinea-pigs Following Intranasal Mycoplasma pneumoniae Infection and Immunization with the 168 kDa Adherence Protein

Contact Info

Product

Resources

About