Humoral and Cellular Immune Responses in Different Rat Strains on Oral Exposure to Ovalbumin

Knippels, L.M.J.; Penninks, André; Meeteren, Masja van; Houben, Geert F.

doi:10.1016/s0278-6915(99)00064-2

Cited by 61 publications

(42 citation statements)

References 25 publications

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“…We therefore find it justified to use an animal model where allergen exposure may be controlled. The animals used were Brown Norway (BN) rats, a high IgE-responder strain [21,22]. For practical reasons, i.e.…”

Section: Introductionmentioning

confidence: 99%

Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

Kroghsbo

Bøgh

Rigby

et al. 2011

Int Arch Allergy Immunol

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Background: It is not known why some foods sensitizing via the gastrointestinal tract are prevalent allergenic foods and others are not. Eating habits, processing, and the food matrix have been suggested to influence the allergenicity of a given food. Factors related to protein structure, such as stability to digestion, have also been suggested. 7S globulins from peanut, hazelnut, soy, and pea were studied to determine whether related proteins would induce a similar sensitization when removed from their ‘normal’ matrix. Methods: Brown Norway rats (soy tolerant or nontolerant) were immunized i.p. 3 times with 100 µg purified peanut, hazelnut, soy, or pea 7S without adjuvant. Sera were analyzed for specific antibodies by different ELISAs (IgG1, IgG2a, and IgE), inhibition ELISA, and rat basophilic leukemia cell assay. Results: The 4 related 7S globulins induced a response with an almost identical level of specific antibodies, but peanut 7S induced IgE of higher avidity than hazelnut and pea 7S which, again, had a higher avidity than IgE induced by soy 7S. Soy tolerance reduced the functionality of IgE without influencing antibody titers. Conclusions: Although the 4 7S globulins are structurally related allergens, they induce antibodies with different antigen-binding characteristics. Peanut 7S induces IgE of a higher avidity than hazelnut and pea 7S which, again, has a higher avidity than IgE induced by soy 7S. We also show that soy tolerance influences the function of antibodies to peanut 7S. These findings may help explain how antibodies of different clinical significances can develop in different individuals sensitized to the same allergen.

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Section: Introductionmentioning

confidence: 99%

Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

Kroghsbo

Bøgh

Rigby

et al. 2011

Int Arch Allergy Immunol

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“…We used two strains of animals, Brown Norway (BN) rats and B10A mice, that have been reported to be high responders to allergic reactions. The BN rat has been shown to be a high IgE responder and thus resembles atopic humans in terms of the propensity to develop allergic reactions 8) . In addition, the BN rat has a similar profile of allergenicity and epitopes of ovalbumin to that of man and can be sensitized via the oral route 9), 10) .…”

Section: Introductionmentioning

confidence: 99%

Effect of Subchronic Feeding of Genetically Modified Corn (CBH351) on Immune System in BN Rats and B10A Mice.

Teshima¹,

Watanabe²,

Okunuki³

et al. 2002

Journal of the Food Hygienics Society of Japan

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Subchronic animal feeding studies to examine the e#ect on the immune system of genetically modified corn CBH351, which contains the Cry9C protein derived from Bacillus thuringiensis subspecies tolworthi, were conducted in female BN rats and B10A mice.The studies were designed to compare the e#ect of a line of genetically modified corn CBH351 (GM corn) with that of isoline corn (non-GM corn). Heat-treated corn meal was incorporated into the diets of the rats and mice at a concentration of 50ῌ. The study duration was 13 weeks. Growth, food intake, and organ weights of the thymus, spleen, and liver were compared between animals fed the non-GM and GM lines. The histological findings in thymus, spleen, mesenteric lymph nodes, Peyer's patches, small intestines, liver, kidney, and bone marrow, and the presence of Cry9C-specific IgE, IgG, IgG1 and IgA antibodies in serum were also compared. The results showed no significant di#erences in growth, feeding value, or the histological findings in immunity-related organs between the animals fed the GM and non-GM lines. Production of Cry9 C-specific IgE and IgA was not detected in the serum of either group. Production of Cry9C-specific IgG and IgG1 was slightly increased in the 50ῌ GM groups of BN rats. No Cry9C-specific IgG or IgG1 was detected in the serum of BN rats fed the diet containing 5ῌ GM-corn In conclusion, no immunotoxic activity was detected in the GM-corn-fed rats and mice in this subchronic dietary study.

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“…The most commonly used strain is the brown Norway able to produce allergen specific IgE after oral sensitization and able to predict the allergenicity of novel proteins [110,111]. Other strains like Wistar, Hooded Lister, and Piebald Virol Glaxo have been studied, but not commonly used due to the inability to detect quantifiable levels of antigen specific IgE [112].…”

Section: Rodent Models Of Food Allergymentioning

confidence: 99%

Animal Models of Allergic Diseases

Santoro

Marsella

2014

Veterinary Sciences

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Allergic diseases have great impact on the quality of life of both people and domestic animals. They are increasing in prevalence in both animals and humans, possibly due to the changed lifestyle conditions and the decreased exposure to beneficial microorganisms. Dogs, in particular, suffer from environmental skin allergies and develop a clinical presentation which is very similar to the one of children with eczema. Thus, dogs are a very useful species to improve our understanding on the mechanisms involved in people's allergies and a natural model to study eczema. Animal models are frequently used to elucidate mechanisms of disease and to control for confounding factors which are present in studies with patients with spontaneously occurring disease and to test new therapies that can be beneficial in both species. It has been found that drugs useful in one species can also have benefits in other species highlighting the importance of a comprehensive understanding of diseases across species and the value of comparative studies. The purpose of the current article is to review allergic diseases across species and to focus on how these diseases compare to the counterpart in people.

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Humoral and Cellular Immune Responses in Different Rat Strains on Oral Exposure to Ovalbumin

Cited by 61 publications

References 25 publications

Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

Sensitization with 7S Globulins from Peanut, Hazelnut, Soy or Pea Induces IgE with Different Biological Activities Which Are Modified by Soy Tolerance

Effect of Subchronic Feeding of Genetically Modified Corn (CBH351) on Immune System in BN Rats and B10A Mice.

Animal Models of Allergic Diseases

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