Humoral immune response to oral microorganisms in periodontitis

Doty, S L; Lopatin, Dennis E.; Syed, Salam A.; Smith, Frederic N.

doi:10.1128/iai.37.2.499-505.1982

Cited by 88 publications

(47 citation statements)

References 31 publications

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“…Eor practical reawas observed in this study. Similar observa-sons, a simpler test based on 2 or 3 bacteria tions were reported from a small group of may be preferable, and as shown in this healthy adults by Doty et al (1982). In study the sensitivity and specificity were still contrast, Mannsheim et al (1980) observed satisfactory.…”

Section: Discussionsupporting

confidence: 78%

Activity of serum immunoglobulins G, A, and M to six anaerobic, oral bacteria in diagnosis of periodontitis

Tolo

Schenck

1985

J of Periodontal Research

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The serum levels of IgG, IgA, and IgM‐antibodies to extracts of Bacteroides gingivalis, Bacteroides ureolyticus, Capnocytophaga ochracea, Eubacterium saburreum, Fusobacterium nucleatum and Selenomonas sputigena were measured by an enzyme‐linked immunosorbent assay (ELISA) in 50 subjects with a healthy periodontium, 10 patients with gingivitis, and 40 patients with periodontitis. In the healthy subjects, the levels of IgG and IgA‐antibodies to the extracts were positively correlated with age up to 20 years; after this age no further increase was observed in the mean levels of antibodies to the oral bacteria. A large majority of the patients with periodontitis had significantly increased levels of IgG and IgA‐antibodies to one or more of the bacterial extracts. By discriminant analysis of the data refering to all six extracts. 95% of the sera from the periodontitis group received a classification that only included 3% of the sera in the healthy group and 20% of the sera in the gingivitis group. A simpler test based on the IgG and IgA‐antibodies to extracts of B. gingivalis and E. saburreum showed a sensitivity of 75% in detecting sera from the periodontitis group. In this test, 70% of the gingivitis sera and 90% of the sera from the healthy subjects were negative.

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Section: Discussionsupporting

confidence: 78%

Activity of serum immunoglobulins G, A, and M to six anaerobic, oral bacteria in diagnosis of periodontitis

Tolo

Schenck

1985

J of Periodontal Research

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“…Our initial trial to estimate the level of antibodies against fimbriae by an enzyme-linked immunosorbent assay, a quantitative assay, also showed a similar tendency (data not shown). The relation between age and the levels of antibodies against fimbriae should be elucidated to confirm the present notion because of the difference of mean age between HI and AP groups, although we take note of reports stating that no significant changes in the mean levels of antibodies against oral microorganisms including B. gingivalis were observed (1,14). In contrast, antibodies against the type 1 fimbriae of Escherichia coli are known to be commonly present in human sera from adults without dysentery (2).…”

supporting

confidence: 66%

Detection of Specific Antibodies against Fimbriae and Membrane Proteins from the Oral Anaerobe Bacteroides gingivalis in Patients with Periodontal Diseases

Yoshimura

Kawanami

et al. 1987

Microbiology and Immunology

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Sera from a number of patients with periodontal diseases were shown to have specific immunoglobulin G (IgG) antibodies against fimbriae and membrane proteins of Bacteroides gingivalis, a suspected pathogen, by using Western blotting analysis. The sera had a strong tendency to react with fimbriae, or exactly oligomeric structures of fimbriae with a native β‐structure rich‐conformation. However, the sera did not react with fimbrilin, a constituent protein of fimbriae, which is denatured by sodium dodecyl sulfate.

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“…In the present study, IgG concentrations towards a specific gingivitis pathogen, A. viscosus, increased (in 5 of 6 subjects) in the healthy post-treatment period compared to the pretreatment gingivitis period. This is in accordance with Doty et al (1982) who reported increased serum antibody titers to certain periodontopathic bacteria in healthy controls compared to diseased subjects. They concluded that in diseased tissues, antibodies may be consumed by interaction with antigens in excess, thus lowering the local antibody concentration.…”

Section: Discussionsupporting

confidence: 93%

Fluctuations in crevicular and salivary anti‐A viscosus antibody levels in response to treatment of gingivitis

Tyneuus-Bratthall

Ellen

1985

J Clinic Periodontology

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Experimental gingivitis studies support a pathogenic rôle for Actinomyces viscosus because of its numerical predominance in disease-associated plaques. The aims of the present investigation were to quantify specific crevicular IgG against A. viscosus before and after conventional gingivitis treatment and to determine whether salivary IgA and IgG against A. viscosus and A. naeslundii would be affected concomitantly. 6 subjects with generalized gingivitis were selected. Examinations were made before and after treatment and included collection of unstimulated saliva, paraffin-stimulated saliva and crevicular material as well as measurements of clinical parameters. The immunoglobulins were estimated by an ELISA assay using whole bacterial cells as antigen. Crevicular IgG against A. viscosus WVU 627 was demonstrated in pre- and post-treatment samples with a tendency towards increased values in the post-treatment samples. Salivary IgA and IgG against A. viscosus were also demonstrated in pre- and post-treatment samples. There were tendencies towards increased IgA values for paraffin-stimulated saliva and increased IgG values for unstimulated saliva in the post-treatment samples. Salivary IgA and IgG values against A. naeslundii ATCC 12104 were either not detected or barely detectable in both pre- and post-treatment samples. A. naeslundii B 74 IgG values were also rather low. As for A. viscosus WVU 627, anti-B 74 IgA values demonstrated a post-treatment increase for most subjects, especially for unstimulated saliva for which all post-treatment values were elevated. It is interesting to note that saliva samples showed changes similar to crevicular material, considering that salivary IgA is most likely derived from a different source. Together, elevated crevicular and salivary antibody concentrations might account for some of the beneficial effects of periodontal therapy by scaling.

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Humoral immune response to oral microorganisms in periodontitis

Cited by 88 publications

References 31 publications

Activity of serum immunoglobulins G, A, and M to six anaerobic, oral bacteria in diagnosis of periodontitis

Activity of serum immunoglobulins G, A, and M to six anaerobic, oral bacteria in diagnosis of periodontitis

Detection of Specific Antibodies against Fimbriae and Membrane Proteins from the Oral Anaerobe Bacteroides gingivalis in Patients with Periodontal Diseases

Fluctuations in crevicular and salivary anti‐A viscosus antibody levels in response to treatment of gingivitis

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