Humoral immune response within the lung in HIV-1 infection

Moja, Ph.; Jalil, Abdul; Quesnel, Anne; Perol, M; Cotte, Laurent; Livrozet, Jean-Michel; Boibieux, A.; Chamson, André; Vergnon, J.M.; Lucht, F.; Tran, Robert K.; Pozzetto, Bruno; Genin, Christian

doi:10.1046/j.1365-2249.1997.4231441.x

Cited by 14 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

Section: Discussionmentioning

confidence: 98%

“…Increased levels of IgG have also been identified in blood [59] of HIV1 women compared to low-risk subjects. Similar patterns of Ig appear in the lung [59] and the female LGT [58], with increased IgG compared to static or reduced sIgA levels. Studies on intestinal mucosa have suggested that this unexpected predominance of IgG over IgA may result from abnormal Ig production in the mucosa rather than from serum leakage [60].…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Ahmed¹,

Al-Doujaily²,

Johnson

et al. 2001

Scand J Immunol

View full text Add to dashboard Cite

This study investigates the distribution of immunocompetent cells in the ectocervix, and cytokine and immunoglobulin (Ig) levels in cervicovaginal secretions to determine whether they are altered in asymptomatic human immunodeficiency virus (HIV) infection. Ectocervical biopsies from 10 HIV1 and 10 presumed HIV-ve women were studied by immunocytochemistry. Levels of Igs in cervicovaginal secretions were quantified by radial immunodiffusion (RID) and cytokine levels by ELISA. HIV1 women had significantly increased numbers of CD8 1 lymphocytes resulting in reversal of the CD4:CD8 ratio. There was a significant increase in the proportion of activated CD81 HLA-DR1 and CD4 1 HLA-DR 1 lymphocytes, but not in CD81 TIA-11 cells. The epithelium of the cervix from HIV1 subjects showed a significant increase in both numbers of macrophages (CD681) and proportions of activated macrophages (CD681 HLA-DR1) compared to normal. The stroma contained increased proportions of inductive (D11) and suppressive (D11 D71) macrophages but a decrease in effector phagocyte (D71) proportions and Langerhans' cells. Significantly lower tumour necrosis factor (TNF)-a levels were observed in cervicovaginal secretions from HIV1 subjects. IgG levels were 4 times higher and IgM levels twice higher in cervicovaginal secretions from HIV1 women, compared to results from normal subjects. These results suggest a response within the CD8 1 cells in HIV1 women, yet these cells may have a low cytolytic capacity. The raised proportions of HLA-DR1 and D11 CD4 1 macrophages could act as antigen-presenting cells (APC) for CD4 1 CD45RO1 lymphocytes, and represent a local acquired response. However, the close juxtaposition of these cells offers the potential for them to act as a local reservoir of virus and promote its proliferation. The increase of IgG over sIgA in secretions of HIV1 subjects provides evidence suggesting a dysregulation of local humoral immunity.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

Immunity in the Female Lower Genital Tract and the Impact of HIV Infection

Ahmed¹,

Al-Doujaily²,

Johnson

et al. 2001

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…An immediate freezing of sampled BAL was performed to avoid urea splitting by organisms potentially present in BAL. Using this approach, we were able to determine the initial concentration of Igs in the ELF recovered by bronchoalveolar lavage (24,30). To determine the concentration of urea recovered from BAL and serum samples, a commercially available kit (Biomerieux, Marcy l'Etoile, France) was used.…”

Section: Methodsmentioning

confidence: 99%

Decreased Production of Local Immunoglobulin A toPneumocystis cariniiin Bronchoalveolar Lavage Fluid from Human Immunodeficiency Virus-Positive Patients

Jalil¹,

Moja²,

Lambert³

et al. 2000

Infect Immun

View full text Add to dashboard Cite

An enzyme-linked immunosorbent assay and a Western blot analysis were developed to study the antibody response to Pneumocystis carinii in serum and bronchoalveolar lavage fluid from 27 human immunodeficiency virus 27 (HIV)-infected patients with P. carinii pneumonia (Pcp), 32 patients without Pcp, and 51 HIV-negative controls. Urea was used for the correct dilution of epithelial lining fluid, and albumin was used to evaluate transudation from plasma for the assessment of local production of antibodies to P. carinii. By contrast with those of immunoglobulin G (IgG), IgA responses to P. carinii were increased in serum from HIV-positive patients compared to negative controls. Local production of antibodies to P. carinii, especially IgA, was decreased in patients with Pcp. In a study of 10 patients of each group, IgG and IgA responses to gp116 from P. carinii were lower in patients with Pcp than in other groups. These results suggest that, in addition to alveolar macrophages, local antibodies may play a role in host defense against P. carinii.

show abstract

“…6 A substantial dysfunction of humoral immunity associated with HIV infection appears to predispose to bacterial pneumonia in these patients. 7 Previous investigators have demonstrated that penicillin is cost-effective in the treatment of lobar pneumonia before the outbreak of HIV infection in Africa. 8,9 A recent paper from India also emphasized the need for a cost-effective approach to treatment of CAP, for example with the use of parenteral penicillin in developing countries.…”

Section: Introductionmentioning

confidence: 99%