Humoral immunity and reduced periodontal bone loss in Eikenella corrodens-monoassociated rats

Behling, Ulrich H.; Sallay, C; Sanavi, F.; Pham, P H; Nowotny, Alois

doi:10.1128/iai.33.3.801-805.1981

Cited by 22 publications

(11 citation statements)

References 20 publications

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“…In our laboratory, Johnson et al (1978) observed accelerated bone destruction in genn-fi^e, irradiated, immunosuppressed rats monoinfected with Eikenella corrodens as compared to nonimmunosuppressed controls, suggesting that absence of immune response does not prevent bone resorption. In agreement with these, our findings indicate that the lack of proper immune response permits the alveolar bone resorption in the mono-infected germ-free rat system (Johnson et al 1978, Behling, Pham & Nowotny 1979 while active immunization prevents its development (Behling et al 1981). Results of other laboratories support the protective effect of immunization (Guggenheim & Schroeder 1974, Rylander, Lindhe & Ahlstedt 1976, Crawford, Taubman & Smith 1978b.…”

Section: Introducttonsupporting

confidence: 88%

Alveolar bone destruction in the immunosuppressed rat

Sallay

Sanavi

Ring

et al. 1982

J of Periodontal Research

100

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The placement of a ligature around the second maxillary molar of the conventional rat caused osteoclastic bone resorption and simultaneously, alveolar bone formation. The number of peripheral mononuclear cells in the blood and lymphoblastic transformation of spleen cells in response to concanavalin A increased. Cyclophosphamide (CY), an immunosuppressive agent, given shortly after placing the ligature suppressed the lymphoid reactions, spleen size, and bone formation and enhanced bone destruction. CY given in higher doses also suppressed the number of PMN cells. Septicemia developed in several of these animals. Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli were isolated from the blood and/or ligature. Antibiotics prevented bone destruction. Without placing a ligature, the high dose of CY did not result in bone loss. These findings suggest that 1) bone destruction of the ligature‐treated rat is of bacterial origin, 2) CY suppresses proliferation of osteoblasts but does not seem to interfere with the activity of osteoclasts, and 3) suppression of the host defenses greatly facilitates bone destruction.

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Section: Introducttonsupporting

confidence: 88%

Alveolar bone destruction in the immunosuppressed rat

Sallay

Sanavi

Ring

et al. 1982

J of Periodontal Research

100

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“…In particular, it is noteworthy that rats immunized with 10'" cells and having a high serum antibody level against B. gingivalis fitnbriae at the time of infection did not show evidence of periodontal destruction by any ofthe 4 tnethods. Cell-tnediated immunity was not assayed in this study, but in a previous investigation no obvi-ous association was found between disease severity and spleen cell blastogenesis to the microorganism used for immunization and infection (2).…”

mentioning

confidence: 74%

Periodontal bone level and gingival proteinase activity in gnotobiotic rats immunized with Bacteroides gingivalis

Klausen

Evans

Ramamurthy

et al. 1991

Oral Microbiology and Immunology

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Bacteroides gingivalis is associated with various forms of periodontal disease. To assess the role of the immune response in modulating B. gingivalis-associated periodontal disease, the effect of immunization of B. gingivalis-induced periodontal bone loss was evaluated in gnotobiotic rats. Male Sprague-Dawley rats immunized with various doses of whole cells or sham-immunized with incomplete Freund's adjuvant were monoinfected with B. gingivalis in carboxymethylcellulose by gavage. Two additional groups served as either sham-immunized or untreated germ-free controls. Forty-two days after infection, all rats were killed, periodontal bone level was assessed morphometrically and radiographically, and gingival proteinase (mammalian collagenase and acid cathepsin) activity was assessed biochemically. B. gingivalis was present in oral samples from all monoinfected rats, and no contaminating bacteria were detected in any oral or fecal sample. Animals immunized with B. gingivalis cells had elevated serum and saliva antibodies to whole cells and partially purified fimbriae from B. gingivalis. Infected sham-immunized rats had significantly more periodontal bone loss than noninfected controls, whereas the periodontal bone level in infected rats immunized with 10(10) B. gingivalis cells was similar to that of the noninfected controls. The activities of gingival collagenase and cathepsin B and L were high in sham-immunized infected rats and low in all other animal groups. In conclusion, it is possible to reduce B. gingivalis-induced periodontal tissue loss in gnotobiotic rats by immunization.

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“…It was suggested that these findings support a hypersensitivity reaction in the progression of disease; however, under normal conditions suppressor mechanisms maintain a stable host-parasite interaction. Parenteral immunization of rats with whole bacterial sonicate elicited significantly elevated antibodies in a germ-free rat model system that developed alveolar bone loss following monoassociation with E. corrodens (5). The immunized rats showed less bone loss than infected controls.…”

Section: Actinomycetemcomitans-sensitized T Cells Transferred Into Otmentioning

confidence: 97%

“…gingivulis in- T cell clones increased antibody Protection Burckhardt et al (11) Immunized l? gingivalis Protection Behling et al (5) Immunized (17) immunized mice with heatkilled l? gingivalis A7A1-28, €?…”

Section: Actinomycetemcomitans-sensitized T Cells Transferred Into Otmentioning

confidence: 99%