Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays

Schaich, Karen M.; Tian, Xin; Xie, Mingyong

doi:10.1016/j.jff.2015.01.043

Cited by 434 publications

(318 citation statements)

References 45 publications

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“…•+ is formed using a potassium persulphate as an oxidizing agent and loses its color in the presence of radical antioxidant [32]. The radical scavenging activity results are presented in Table 3, In DPPH• assay, all extracts of C. pachysporus showed high activity while all extract of S. tenuipes displayed low Research Article activity (Table 3).…”

Section: Dpphmentioning

confidence: 99%

Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey

Deveci¹,

Çayan²,

Duru³

et al. 2018

mpj

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ABSTRACT:The aim of the present study was to compare phytochemical composition and biological activities of various extracts of two Basidiomycota truffle collected from Turkey. Analysis of phytochemical compositions in terms of phenolic and fatty acid compositions was determined using HPLC-DAD, GC and GC/MS. Fumaric (10.62, 4.12 µg/g) and protocatechuic acids (2.71, 20.50 µg/g) were identified as the major organic and phenolic acids in Chondrogaster pachysporus and Setchelliogaster tenuipes truffles. Linoleic acid (53.03 %) for C. pachysporus and palmitic acid (38.60 %) for S. tenuipes were found to be the most dominant fatty acids. The extracts were also tested for their antioxidant and anticholinesterase activities. As for antioxidant and anticholinesterase activity results, C. pachysporus truffle showed better activity than S. tenuipes. This work presents the first phytochemical study on C. pachysporus and S. tenuipes truffles and sheds light on their potential usage as the natural source in the food, pharmaceutical, and cosmetic industries.

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Section: Dpphmentioning

confidence: 99%

Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey

Deveci¹,

Çayan²,

Duru³

et al. 2018

mpj

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show abstract

“…Schaich et al 39 mentioned several factors that limit the use of the ABTS and DPPH tests, such as disregard for chemical aspects, including the effects of solvents, concentration, medium pH, radical solubility and, particularly, reaction kinetics; use of stable radicals with stereochemical with hindrance instead of easy access and short duration small radicals (example: OH…”

Section: Resultsmentioning

confidence: 99%

“…Oliveira et al 4 described the ascorbic acid as an interfering agent in the evaluation of the antioxidant capacity of anthocyanins in foods. Schaich et al 39 cited the interference caused by the oxygen dissolved in the medium and the use of organic solvents (for instance, methanol in the DPPH test).…”

Section: Resultsmentioning

confidence: 99%

Applicability of a Voltammetric Assay Based on the Electroreduction of Oxygen to Evaluate the Antioxidant Capacity of Pequi (Caryocar brasiliense Camb.) Pulp

Pinheiro¹,

Okumura²,

Silva³

et al. 2018

J. Braz. Chem. Soc.

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This study aimed to use a differential pulse voltammetric (DPV) assay, based on the electroreduction of oxygen, to evaluate the antioxidant capacity of pequi pulp. The results were expressed in coefficient of antioxidant capacity (K), at different pH values and in trolox equivalent antioxidant capacity (TEAC), and were compared to the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assays. The principal component analysis showed higher K for pequi pulp extract and pH tending to 7.15. The antioxidant capacity was related to the presence of gallic acid in its composition. Regarding the TEAC value, the best result was obtained by the DPV test (53.43 ± 5.14 µmol trolox g -1 pulp). The pequi pulp showed antioxidant capacity, compared to the standard antioxidant compounds evaluated. It was concluded that the voltammetric protocol was efficient to assess the total antioxidant capacity of pequi pulp and can be an alternative to conventional spectrophotometric assays, for the analysis of food matrices.

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“…Both DPPH and ABTS assays are widely used to assess the antioxidant capacity of natural products (Schaich et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Phytochemical screening, total phenolic content and antioxidant activity of some plants from Brazilian flora

Neto¹,

Uchôa²,

Moura³

et al. 2016

J. Med. Plants Res.

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The present study evaluated the total phenolic and flavonoid content as well as the antioxidant activity of methanolic leaf extracts of five plants from Brazilian flora: Abarema cochliacarpos, Croton corchoropsis, Myroxylon peruiferum, Stryphnodendron pulcherrimum and Tanaecium cyrtanthum by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant capacity assays. A thin layer chromatography analysis of all plant extracts has also been performed and it showed the presence of different types of secondary metabolites, namely saponins, phenylpropanoids and flavonoids. Among the studied plants, A. cochliacarpos and S. pulcherrimum showed considerable antioxidant radical scavenging activity on all the tested assays and they also exhibited substantial amounts of phenolic compounds. In addition, a positive correlation was found between total phenols and both ABTS radical scavenging activity and total antioxidant capacity assays, thus indicating the major role of phenols on the antioxidant activity of these plants. To the best of the authors' knowledge, this is the first approach where the phenolic content and antioxidant activity of A. cochliacarpos, C. corchoropsis, M. peruiferum, S. pulcherrimum and T. xanthophyllum were explored.

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Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays

Cited by 434 publications

References 45 publications

Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey

Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey

Applicability of a Voltammetric Assay Based on the Electroreduction of Oxygen to Evaluate the Antioxidant Capacity of Pequi (Caryocar brasiliense Camb.) Pulp

Phytochemical screening, total phenolic content and antioxidant activity of some plants from Brazilian flora

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