Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea

Abstract

A moderately halophilic Gram-staining-negative bacterium, designated strain Y26T, was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26T was observed at 15–35 °C (optimum 25–30 °C), pH 6.0–8.0 (optimum pH 6.5–7.5) and with 1.5–6.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant fatty acids were C18 : 1 ω7c (66.2 %), C16 : 0 (12.4 %) and C10 : 0 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26T contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26T formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8–94.6 % sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26T represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26T (=KACC 13705T =DSM 22009T).

Maritimibacter lacisalsi sp. nov., isolated from a salt lake, and emended description of the genus Maritimibacter Lee et al. 2007

“…The fatty acid profiles of strain X12M-4 T and M. alkaliphilus CGMCC 1.7698 T are shown in . The cellular fatty acid profile obtained in this study for M. alkaliphilus CGMCC 1.7698 T was similar to that in the original description (Lee et al , 2007) and reported in other publications (Kim et al , 2010; Lee et al , 2007; Yoon et al , 2009) in terms of major fatty acids, although there were differences in proportions, which might be due to the different culture conditions. Strain X12M-4 T had a fatty acid profile similar to that of M. alkaliphilus CGMCC 1.7698 T under the same cultivation conditions ().…”

Shewanella aestuarii sp. nov., a marine bacterium isolated from a tidal flat

“…It has long been known that tidal flats contain a wealth of valuable biological resources such as micro-organisms and marine animals and play important roles in the restoration of coastal ecosystems and nutrient cycling (Winberg et al , 2007; Jin et al , 2012; Math et al , 2012). In the course of studies investigating micro-organisms inhabiting tidal flats, we have isolated and characterized many novel bacterial species (Kim et al , 2010; Jin et al , 2011; Lee et al , 2011; Jeong et al , 2013a, b). Here, we describe the taxonomic characterization of a novel species belonging to the genus Shewanella isolated from a tidal flat of the South Sea in South Korea.…”

Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia

“…Members of the family Rhodobacteraceae are metabolically and physiologically diverse and occupy a wide range of niches in various habitats, such as seawater, saline, sediments, sea ice, microbial mats, seaweeds and marine animal tissues. Several genera, including Maribius (Choi et al , 2007), Marivita (Hwang et al , 2009), Mameliella (Wang et al , 2010), Roseovarius (Zheng et al , 2010) and Hwanglaeicola (Kim et al , 2010), have been described in the last 4 years. In this study, a novel and slightly halophilic strain, designated KAUST-100406-0324 T , was isolated from the sediment near a deep-sea brine pool in the Red Sea and characterized using a polyphasic approach.…”