Hyaluronan affects protein and collagen synthesis by in vitro human skin fibroblasts

Croce, Maria Antonietta; Dyne, Katharine M.; Boraldi, Federica; Quaglino, Daniela; Cetta, Giuseppe; Tiozzo, Roberta; Ronchetti, Ivonne Pasquali

doi:10.1054/tice.2001.0180

Cited by 85 publications

(54 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its persistence at sites of wounding or tissue injury, however, is associated with progressive fibrosis and scarring in a number of organs including the lung and the kidney (5,11,42,43). The data presented suggest that as a result of the differentiation of resident fibroblasts to a myofibroblastic phenotype, HA accumulates and may be a major contributor to the maintenance of the cells in that phenotype.…”

Section: Discussionmentioning

confidence: 85%

Myofibroblastic Differentiation Leads to Hyaluronan Accumulation through Reduced Hyaluronan Turnover

Jenkins

Thomas

Williams

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

During the initiation and progression of fibrosis there is extensive differentiation of cells to a myofibroblastic phenotype. Because the synthesis of hyaluronan (HA) was recently linked to oncogenic epithelial-mesenchymal transformation, the present study investigated whether increased HA synthesis was also associated with myofibroblastic differentiation. HA synthesis and size were measured by incorporation of 3 H]HA at a slower rate than fibroblasts and expressed lower levels of both HYAL 1 and HYAL 2 mRNA. Immunoblotting revealed more HYAL 1 and 2 in the myofibroblast conditioned medium. After acidification, however, there was no difference in HA degradation. This suggests that much of the released HYAL is inactive and that the observed differences in HA degradation are caused by cell-associated rather than secreted activity. This was confirmed by immunohistochemical staining for HYAL 1 and HYAL 2. This finding indicates the potential importance of the HYAL enzymes in controlling fibrotic progression and contrasts HA synthesis as a mediator of oncogenic transformation with that of HA degradation controlling fibrogenic differentiation.

show abstract

Section: Discussionmentioning

confidence: 85%

Myofibroblastic Differentiation Leads to Hyaluronan Accumulation through Reduced Hyaluronan Turnover

Jenkins

Thomas

Williams

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Many applications that take advantage of the exceptionally high viscosity characteristics to facilitate a thickened gel-like barrier were first found almost indispensable for surgery procedures in ophthalmology, such as intra-ocular lenses implantation. Other uses of hyaluronan were reported for the treatment of degenerative joint dysfunction in horses and gradually expanded to the experimental treatment to humans [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Influences of hyaluronan on type II collagen fibrillogenesis in vitro

Kuo

Wang²,

Niu

et al. 2007

J Mater Sci: Mater Med

View full text Add to dashboard Cite

The effect to the kinetics of type II collagen fibrillogenesis with the addition of hyaluronan (HA), (M w of 1.8 · 10 6 Da), at various concentrations of HA (0.01, 0.05 and 0.1 wt.%) for a series of fibril formation systems was examined in this study. Evidences deduced from the turbidity-time curves revealed that the inclusion of HA had minor or no impact to the fibrillogenesis of type II collagen (collagen conc. at 0.2 mg/mL). The apparent rate constants, k lag (lag phase) increased slightly but k g (growth phase) decreased not very significantly with addition of HA, as compared to the case of pure collagen. This leads us to believe tentatively that, with the addition of HA to collagen solutions, the nucleation process of the fibril formation might have been sped up slightly whereas the growth process slowed up slightly. However, data from TEM observations on the resulting fibrils indicated that the presence of HA did not significantly affect the diameters and the characteristic D-banding periods of the collagen fiber formed. And, from the statistical analyses, we found only insignificant difference (P > 0.05) between the specimens from the various experimental groups. It seems to indicate that the ultimate packing of collagen monomers was probably not interfered or affected significantly by the presence of HA in vitro.

show abstract

“…Molecular weight and concentration, as well as physical form (i.e., soluble, immobilized, or incorporated) seem to be important variables in regulating HA-dependent effects on a particular end-point. [28][29][30][31][32] Interestingly, several studies have demonstrated the potential for HA to effect both the expression and activation of MMPs in other in vitro models. [33][34][35] Studies with human corneal keratocyte and skin fibroblast explant cultures have demonstrated that administration of high molecular weight HA causes a significant activation of latent MMP-9 as well as MMP-2.…”

Section: Discussionmentioning

confidence: 99%

Development of a Model Bladder Extracellular Matrix Combining Disulfide Cross‐Linked Hyaluronan with Decellularized Bladder Tissue

Brown

Srokowski

Shu

et al. 2006

Macromolecular Bioscience

View full text Add to dashboard Cite

[Image: see text] In this work we investigate the feasibility of modifying porcine-derived BAM to include HA with a view to developing a model, artificial extracellular matrix for the study of bladder cell-matrix interactions. HA-DPTH was incorporated into BAM disks and then cross-linked oxidatively to a disulfide containing hydrogel. Disks were seeded with bladder smooth muscle cells (BSMC) and UEC under three culture configurations and incubated for 3, 7, and 14 d. At each time point, matrix contraction was measured, and media supernatants assayed for cell-secreted gelatinase activity. To evaluate cell adherence and organization, triple immunofluorescent labeling of cell nuclei, actin cytoskeleton, and focal contacts was performed. HA-modified BAM exhibited a significant increase in matrix contraction and induced a higher level of cell-secreted gelatinase activity compared to unmodified BAM. Immunofluorescent labeling demonstrated that BSMCs remained adherent to both scaffold types over time. The distribution and organization of the cytoskeleton and focal contacts did not appear to be altered by the presence of HA. Interestingly, cellular infiltration into modified BAM was evident by 7 d and continued beyond 14 d, while BSMCs seeded onto unmodified BAM remained localized to the surface out to 14 d, with minimal infiltration evident only at day 28. These differences in cell infiltration support the gelatinase activity results. Increases in cell migration and matrix proteolysis in the presence of HA may be contributing factors toward BAM remodeling leading to increased matrix contraction with time. The model ECM developed in this work will be utilized for future studies aimed at elucidating the mechanisms controlling key remodeling events associated with bladder repair. Matrix contraction of cell-seeded BAM scaffolds.

show abstract

Hyaluronan affects protein and collagen synthesis by in vitro human skin fibroblasts

Cited by 85 publications

References 29 publications

Myofibroblastic Differentiation Leads to Hyaluronan Accumulation through Reduced Hyaluronan Turnover

Myofibroblastic Differentiation Leads to Hyaluronan Accumulation through Reduced Hyaluronan Turnover

Influences of hyaluronan on type II collagen fibrillogenesis in vitro

Development of a Model Bladder Extracellular Matrix Combining Disulfide Cross‐Linked Hyaluronan with Decellularized Bladder Tissue

Contact Info

Product

Resources

About