Hyaluronan and Fibrin Biomaterial as Scaffolds for Neuronal Differentiation of Adult Stem Cells Derived from Adipose Tissue and Skin

Abstract

Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes.

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

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Tissue Engineering Strategies as Tools for Personalized Meningioma Treatment

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“…This has had a huge impact, further improving the authenticity of in vitro tumor models. All these devices have been used in several medical applications for tissue repair, controlled drug release, nerve regeneration, and as wound dressings, or in microparticles easily enriched with active proteins (i.e., growth factors) for drug delivery application (8,(39)(40)(41). These heterologous multicompartment tumor co-cultures allow cellular analysis in a controlled and reproducible format as well as providing a realistic portrayal of the in vivo response to experimental treatment.…”

Effect of ethylenediaminetetraacetic acid and hyaluronic acid on the viability and cytokine expression of periodontal ligament fibroblasts

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