Hyaluronan/colistin polyelectrolyte complexes: Promising antiinfective drug delivery systems

Dubashynskaya, Natallia V.; Raik, Sergei V.; Dubrovskii, Yaroslav A.; Shcherbakova, Elena S.; Demyanova, Elena; Shasherina, Anna Yu.; Anufrikov, Yuri A.; Poshina, Daria N.; Добродумов, А. В.; Skorik, Yury A.

doi:10.1016/j.ijbiomac.2021.07.114

Cited by 22 publications

(11 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The positively charged CT molecules are able to interact with polyanionic HA molecules and form PECs. In addition, the CT molecule contains a lipophilic fatty acyl tail and a hydrophilic head group; therefore, CT can form micellar structures with anionic polymers that are further stabilized by the addition of chitosan and chitosan derivatives [6,17,21]. The use of this type of polyelectrolyte particle as a systemic circulation drug carrier requires that the particles have a size below 300 nm and a negative charge below −15 mV to ensure stability.…”

Section: Preparation Optimization and Characterization Of Ct-ha-deaecs Pecsmentioning

confidence: 99%

“…LC-MS was performed as previously described [21]. Chromatographic separation was performed using an Elute UHPLC (Bruker Daltonics GmbH, Bremen, Germany) equipped with a Millipore Chromolith Performance/PR-18e, C18 analytical column (100 mm × 2 mm, Merck, Darmstadt, Germany) with Chromolith ® RP-18 endcapped 5-3 guard cartridges (Merck, Darmstadt, Germany).…”

Section: Liquid Chromatography-mass Spectrometry (Lc-ms) Measurementsmentioning

confidence: 99%

“…As a cationic peptide antibiotic, CT has the ability to interact with anionic molecules, including anionic polymers, and to form negatively charged NPs, and the resulting complexes can be used as carriers for the targeted delivery of CT [6,[17][18][19][20]. For example, our research group [21] studied the formation of CT delivery systems formed by complexation of the drug with HA via electrostatic interaction. The resulting particles had a negative surface charge (−19 mV) and ranged in size from 200 nm to 1 µm, depending on the ratio of HA/CT and the molecular weight (MW) of HA.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Dubashynskaya

Raik

Dubrovskii

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Improving the therapeutic characteristics of antibiotics is an effective strategy for controlling the growth of multidrug-resistant Gram-negative microorganisms. The purpose of this study was to develop a colistin (CT) delivery system based on hyaluronic acid (HA) and the water-soluble cationic chitosan derivative, diethylaminoethyl chitosan (DEAECS). The CT delivery system was a polyelectrolyte complex (PEC) obtained by interpolymeric interactions between the HA polyanion and the DEAECS polycation, with simultaneous inclusion of positively charged CT molecules into the resulting complex. The developed PEC had a hydrodynamic diameter of 210–250 nm and a negative surface charge (ζ-potential = −19 mV); the encapsulation and loading efficiencies were 100 and 16.7%, respectively. The developed CT delivery systems were characterized by modified release (30–40% and 85–90% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro experiments showed that the encapsulation of CT in polysaccharide carriers did not reduce its antimicrobial activity, as the minimum inhibitory concentrations against Pseudomonas aeruginosa of both encapsulated CT and pure CT were 1 μg/mL.

show abstract

Section: Preparation Optimization and Characterization Of Ct-ha-deaecs Pecsmentioning

confidence: 99%

Section: Liquid Chromatography-mass Spectrometry (Lc-ms) Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Dubashynskaya

Raik

Dubrovskii

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…PMXs are positively charged, so they can interact with anionic phospholipids and anionic polymers; therefore, the modern delivery systems considered for PMXs include anionic liposomes [ 8 , 9 , 10 ] and anionic polymers. The anionic polymers form polyelectrolyte complexes (PECs) with PMXs due to electrostatic interactions [ 11 , 12 ]. For instance, Liu et al reported the preparation of nanoparticles (NPs) by complexation of colistin (polymyxin E) with poly(glutamic acid) and further stabilization of this delivery system using 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy (polyethylene glycol)-2000) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, Liu et al reported the preparation of nanoparticles (NPs) by complexation of colistin (polymyxin E) with poly(glutamic acid) and further stabilization of this delivery system using 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy (polyethylene glycol)-2000) [ 11 ]. Dubashynskaya et al evaluated the efficiency of hyaluronan/colistin PECs as PMX delivery systems [ 12 ]. The positively charged PMXs can also be successfully loaded into PECs formed by combinations of polycations and polyanions.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics

Iudin

Vasilieva

Knyazeva

et al. 2022

IJMS

View full text Add to dashboard Cite

The growing number of drug-resistant pathogenic bacteria poses a global threat to human health. For this reason, the search for ways to enhance the antibacterial activity of existing antibiotics is now an urgent medical task. The aim of this study was to develop novel delivery systems for polymyxins to improve their antimicrobial properties against various infections. For this, hybrid core–shell nanoparticles, consisting of silver core and a poly(glutamic acid) shell capable of polymyxin binding, were developed and carefully investigated. Characterization of the hybrid nanoparticles revealed a hydrodynamic diameter of approximately 100 nm and a negative electrokinetic potential. The nanoparticles demonstrated a lack of cytotoxicity, a low uptake by macrophages, and their own antimicrobial activity. Drug loading and loading efficacy were determined for both polymyxin B and E, and the maximal loaded value with an appropriate size of the delivery systems was 450 µg/mg of nanoparticles. Composite materials based on agarose hydrogel were prepared, containing both the loaded hybrid systems and free antibiotics. The features of polymyxin release from the hybrid nanoparticles and the composite materials were studied, and the mechanisms of release were analyzed using different theoretical models. The antibacterial activity against Pseudomonas aeruginosa was evaluated for both the polymyxin hybrid and the composite delivery systems. All tested samples inhibited bacterial growth. The minimal inhibitory concentrations of the polymyxin B hybrid delivery system demonstrated a synergistic effect when compared with either the antibiotic or the silver nanoparticles alone.

show abstract

Chemical modification of hyaluronic acid as a strategy for the development of advanced drug delivery systems

Bokatyi,

Dubashynskaya,

Skorik

2024

Carbohydrate Polymers

View full text Add to dashboard Cite

Hyaluronan/colistin polyelectrolyte complexes: Promising antiinfective drug delivery systems

Cited by 22 publications

References 66 publications

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Hybrid Nanoparticles and Composite Hydrogel Systems for Delivery of Peptide Antibiotics

Chemical modification of hyaluronic acid as a strategy for the development of advanced drug delivery systems

Contact Info

Product

Resources

About