Hyaluronan in synovial fluid of patients with loose total hip prosthesis

Yamada, Hiroshi; Morita, Mitsuhiro; Henmi, Osamu; Miyauchi, Satoshi; Yoshida, Yuko; Kikuchi, Toshiyuki; Terada, Nobuki; Washimi, Osuke; Washimi, Yuki; Seki, Tomohiro

doi:10.1007/s004029900131

Cited by 15 publications

(10 citation statements)

References 3 publications

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“…was conducted at 37°C for 5 million cycles, with the have been shown to be higher than the molecular lubricants being changed every 24 hours (~84 600 weight profiles of the osteoarthritic fluid (1.12±0.84 cycles). Wear rates of the UHMWPE tibial compo-×106 Daltons) [19,22]. Using these reported values, nents were determined using a gravimetric analysis this study selected an average HA concentration of at 0.5 million cycle intervals.…”

Section: Materials and Methods 23 Simulator Lubricant Formulation 21 Synovial Fluid Collectionmentioning

confidence: 99%

Increased Total Knee Arthroplasty Ultra-High Molecular Weight Polyethylene Wear Using a Clinically Relevant Hyaluronic Acid Simulator Lubricant

DesJardins

Antoniac

Tanner

et al. 2006

Proc Inst Mech Eng H

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In this study, osteoarthritic and periprosthetic synovial fluid samples were rheologically and biochemically compared to develop a hyaluronic acid (HA) supplemented bovine serum (BS) lubricant that mimicked the properties of human joint synovial fluid. The effect of this BS + HA lubricant (50 per cent bovine calf serum + 1.5 g/l HA) on the wear rate of ultra-high molecular weight polyethylene (UHMWPE) during a total knee replacement wear test was then investigated. In conjunction with biochemical similarities, the rheological analysis showed that the BS + HA lubricant viscosity was not statistically different to aspirated total knee arthroplasty (TKA) revision joint fluid viscosity over a range of physiologic shear rates. Gravimetric results at 5 million wear testing cycles showed that the BS + HA lubricant produced an average of 6.88 times more UHMWPE wear than 50 per cent bovine serum lubricant alone. The BS + HA lubricated CoCr femoral component surfaces revealed pitting and surface roughening that was not observed using standard bovine serum only lubricants, but that was similar to the metallic surface corrosion observed on in vivo CoCr femoral component retrievals. These findings support the hypothesis that the addition of HA to simulator lubricant is capable of producing CoCr femoral component surface damage similar to that observed in vivo.

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Section: Materials and Methods 23 Simulator Lubricant Formulation 21 Synovial Fluid Collectionmentioning

confidence: 99%

Increased Total Knee Arthroplasty Ultra-High Molecular Weight Polyethylene Wear Using a Clinically Relevant Hyaluronic Acid Simulator Lubricant

DesJardins

Antoniac

Tanner

et al. 2006

Proc Inst Mech Eng H

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“…In Vitro Hyaluronan Synthase Assay-Hyaluronan synthase activity was monitored using a modification of previously described methods (31,32). Briefly, FS, cultured in 15-cm tissue culture dishes, were washed, incubated in hypotonic lysis buffer (LB) (10 min), and harvested into 1 ml of LB supplemented with aprotinin, leupeptin, and phenylmethylsulfonyl fluoride.…”

Section: Reagents-recombinantmentioning

confidence: 99%

“…2) is an indicator that the observed elevated mRNA levels of HAS1 resulted in elevated HA levels, we measured HA synthase activity in untreated as well as TGF-␤-treated cell membrane extracts. An in vitro HA synthase assay was established using published protocols (31,32). FS were grown to high density in 15-cm tissue culture dishes.…”

Section: Mrna Levels Of Has1 Has2 and Has3 In Stimulated Andmentioning

confidence: 99%

Differential Effect of Transforming Growth Factor β (TGF-β) on the Genes Encoding Hyaluronan Synthases and Utilization of the p38 MAPK Pathway in TGF-β-induced Hyaluronan Synthase 1 Activation

Stuhlmeier¹,

Pollaschek²

2004

Journal of Biological Chemistry

108

115

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Unfettered hyaluronan (HA) production is a hallmark of rheumatoid arthritis. The discovery of three genes encoding hyaluronan synthases (HASs) allows for the investigation of the signaling pathways leading to the activation of these genes. Our objective is to further understanding of the regulation of these genes as well as to find ways to prevent undesired gene activation. Human fibroblast-like synoviocytes were used in these experiments. mRNA levels of HAS were monitored by reverse transcriptase-PCR. A series of specific kinase inhibitors were used to investigate intracellular pathways leading to the up-regulation of HAS1. Our experiments, testing a series of stimuli including tumor necrosis factor ␣ (TNF␣), demonstrate that TGF-␤ is the most potent stimulus for HAS1 transcription. TGF-␤ activates HAS1 in a dose-dependent manner with a maximum effect at a concentration of 0.5-1 ng/ml. TGF-␤-induced HAS1 mRNA can be detected within 60 min and reaches maximal levels at 6 h. Furthermore, TGF-␤ treatment leads to an increase in synthase activity as determined by HA ELISA and by in vitro HA synthase assays. In contrast to the activatory effect on HAS1, TGF-␤ dosedependently suppresses HAS3 mRNA. As to the mode of action of TGF-␤-induced HAS1 mRNA activation, our experiments reveal that blocking p38 MAPK inhibited the TGF-␤ effect by 90%, blocking the MEK pathway led to an inhibition by 40%, and blocking the JNK pathway had no effect. The presented data might contribute to a better understanding of the role of TGF-␤ and of HA in the pathology of diseases.

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“…60 Also, the change of sulfation contents in synovial fluid KS is clearly correlated with the severity of osteoarthritis. 61 KS differs from other GAGs by containing a nonacidic residue. This simplifies its chemical synthesis since there is no demand of elaborating C-6 to generate a carboxylate group.…”

Section: Glycosaminoglycansmentioning

confidence: 97%

Carbohydrate post-glycosylational modifications

Chen

2007

Org. Biomol. Chem.

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Carbohydrate modification is a common phenomenon in nature. Many carbohydrate modifications such as some epimerization, O-acetylation, O-sulfation, O-methylation, N-deacetylation, and Nsulfation, take place after the formation of oligosaccharide or polysaccharide backbones. These modifications can be categorized as carbohydrate post-glycosylational modifications (PGMs). Carbohydrate PGMs further extend the complexity of the structures and the synthesis of carbohydrates and glycoconjugates. They also increase the capacity of the biological information that can be controlled by finely tuning the structures of carbohydrates. Developing efficient methods to obtain structurally defined naturally occurring oligosaccharides, polysaccharides, and glycoconjugates with carbohydrate PGMs is essential for understanding the biological significance of carbohydrate PGMs. Combine with high-throughput screening methods, synthetic carbohydrates with PGMs are invaluable probes in structure-activity relationship studies. We illustrate here several classes of carbohydrates with PGMs and their applications. Recent progress in chemical, enzymatic, and chemoenzymatic syntheses of these carbohydrates and their derivatives are also presented.

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Hyaluronan in synovial fluid of patients with loose total hip prosthesis

Cited by 15 publications

References 3 publications

Increased Total Knee Arthroplasty Ultra-High Molecular Weight Polyethylene Wear Using a Clinically Relevant Hyaluronic Acid Simulator Lubricant

Increased Total Knee Arthroplasty Ultra-High Molecular Weight Polyethylene Wear Using a Clinically Relevant Hyaluronic Acid Simulator Lubricant

Differential Effect of Transforming Growth Factor β (TGF-β) on the Genes Encoding Hyaluronan Synthases and Utilization of the p38 MAPK Pathway in TGF-β-induced Hyaluronan Synthase 1 Activation

Carbohydrate post-glycosylational modifications

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