Hyaluronan-mediated angiogenesis in vascular disease: Uncovering RHAMM and CD44 receptor signaling pathways

Slevin, Mark; Krupinski, Jurek; Gaffney, John; Matou, Sabine; West, David C.; DeLisser, Horace M.; Savani, Rashmin C.; Kumar, Shant

doi:10.1016/j.matbio.2006.08.261

Cited by 402 publications

(344 citation statements)

References 97 publications

Supporting

Mentioning

323

Contrasting

Unclassified

Order By: Relevance

“…33 However, low molecular weight hyaluronan oligosaccharides have been shown to promote angiogenesis in several studies. [34][35][36] Halici et al 37 reported that injected sodium hyaluronate increased VEGF and type I collagen expression in a defected achilles-tendon in a rabbit model, suggesting that sodium hyaluronate may be partly involved the regulation of angiogenesis.…”

Section: Composite Silk Scaffold For Tissue-engineered Ligamentsmentioning

confidence: 99%

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Seo

Yoon

Song

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

The purpose of this study was to evaluate the biocompatibility of silk and collagen-hyaluronan (HA) in vitro by assessing anterior cruciate ligament (ACL) cell and T-lymphocyte cultures on scaffolds. The use of composite scaffolds as artificial ligaments in ACL reconstruction and their effects on angiogenesis were evaluated in vivo. The silk scaffold was knitted by hand and dry coated with collagen-HA, whereas the composite silk scaffold was made by covering a silk scaffold with a lyophilized collagen-HA substrate. The initial attachment and proliferation of human ACL cells on the composite silk scaffold was superior to the attachment and proliferation observed on the silk scaffold. The immune response was higher in both scaffolds after 72 h ( p < 0.05) compared with the control culture condition without scaffolding, as assessed by T-lymphocyte cultures in vitro. There was no significant difference in the immune response in vitro between the silk and composite silk scaffolds. Silk and composite silk scaffolds were implanted as artificial ligaments in ACLs removed from the knees of dogs, and they were harvested 6 weeks after implantation. On gross examination, the onset of an inflammatory tissue reaction, such as synovitis, was seen in both the silk scaffold and the composite silk scaffold groups. An histological evaluation of the artificial ligament implants revealed the presence of monocytes in the silk composite scaffold and the absence of giant cells in all cases. MT staining in the composite silk scaffold-grafted group showed granulation tissue consisting of fibroblasts, lymphocytes, monocytes, and collagen fibers. In addition, CD31 staining revealed the formation of new blood vessels. On the other hand, no reparative tissues, such as blood vessels, collagen, and cells, were observed in the silk scaffold-grafted group. These results suggest that the lyophilized collagen-HA substrate is biocompatible in vitro and enhances new blood vessel and cell migration in vivo. ß

show abstract

Section: Composite Silk Scaffold For Tissue-engineered Ligamentsmentioning

confidence: 99%

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Seo

Yoon

Song

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…during embryonic morphogenesis, inflammation and wound repair, as well as in cancer. In these contexts, hyaluronan has an instructive, cell signaling function in addition to its structural role (Jiang et al, 2007;Slevin et al, 2007;Toole, 2001;Toole, 2004;Turley et al, 2002). Though hyaluronan signals through interaction with several cell surface receptors, CD44 is the best characterized of these receptors in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…Though hyaluronan signals through interaction with several cell surface receptors, CD44 is the best characterized of these receptors in cancer cells. Variant forms of CD44 are commonly up-regulated in cancers and CD44 has been implicated in numerous aspects of cancer progression (Hill et al, 2006a;Liu and Jiang, 2006;Marhaba and Zoller, 2004;Slevin et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan, CD44 and Emmprin: Partners in cancer cell chemoresistance

Toole

Slomiany

2008

Drug Resistance Updates

161

140

View full text Add to dashboard Cite

Hyaluronan is not only an important structural component of extracellular matrices but also interacts with cells during dynamic cell processes such as occur in cancer. Consequently, interactions of hyaluronan with tumor cells play important cooperative roles in various aspects of malignancy. Hyaluronan binds to several cell surface receptors, including CD44, thus leading to co-regulation of signaling pathways that are important in regulation of multidrug resistance to anticancer drugs, in particular anti-apoptotic pathways induced by activation of receptor tyrosine kinases. Emmprin, a cell surface glycoprotein of the Ig superfamily, stimulates hyaluronan production and downstream signaling consequences. Emmprin and CD44 also interact with various multidrug transporters of the ABC family and monocarboxylate transporters associated with resistance to cancer therapies. Moreover, hyaluronan-CD44 interactions are critical to these properties in the highly malignant, chemotherapy-resistant cancer stem-like cells. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. These antagonists, especially small hyaluronan oligomers, may be useful in therapeutic strategies aimed at preventing tumor refractoriness or recurrence due to drug-resistant sub-populations within malignant cancers.

show abstract

“…The HA we have immobilized is fluorescently labeled with a molecular weight of 800 kDa. However, HA ranges in size from 2000-25000 repeating disaccharide units, and cellular response has been demonstrated to be dependent on HA molecular weight [10][11] . This protocol can therefore be applied to immobilize HA of varying molecular weights to investigate cell response to exogenous HA.…”

Section: Discussionmentioning

confidence: 99%

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Dickinson

Gerecht

2010

JoVE

View full text Add to dashboard Cite

Cancer invasion and progression involves a motile cell phenotype, which is under complex regulation by growth factors/cytokines and extracellular matrix (ECM) components within the tumor microenvironment. Hyaluronic acid (HA) is one stromal ECM component that is known to facilitate tumor progression by enhancing invasion, growth, and angiogenesis 1 . Interaction of HA with its cell surface receptor CD44 induces signaling events that promote tumor cell growth, survival, and migration, thereby increasing metastatic spread 2-3 . HA is an anionic, nonsulfated glycosaminoglycan composed of repeating units of D-glucuronic acid and D-N-acetylglucosamine. Due to the presence of carboxyl and hydroxyl groups on repeating disaccharide units, native HA is largely hydrophilic and amenable to chemical modifications that introduce sulfate groups for photoreative immobilization [4][5] . Previous studies involving the immobilizations of HA onto surfaces utilize the bioresistant behavior of HA and its sulfated derivative to control cell adhesion onto surfaces [6][7] . In these studies cell adhesion preferentially occurs on non-HA patterned regions.To analyze cellular interactions with exogenous HA, we have developed patterned functionalized surfaces that enable a controllable study and high-resolution visualization of cancer cell interactions with HA. We utilized microcontact printing (uCP) to define discrete patterned regions of HA on glass surfaces. A "tethering" approach that applies carbodiimide linking chemistry to immobilize HA was used 8 . Glass surfaces were microcontact printed with an aminosilane and reacted with a HA solution of optimized ratios of EDC and NHS to enable HA immobilization in patterned arrays. Incorporating carbodiimide chemistry with mCP enabled the immobilization of HA to defined regions, creating surfaces suitable for in vitro applications. Both colon cancer cells and breast cancer cells implicitly interacted with the HA micropatterned surfaces. Cancer cell adhesion occurred within 24 hours with proliferation by 48 hours. Using HA micropatterned surfaces, we demonstrated that cancer cell adhesion occurs through the HA receptor CD44. Furthermore, HA patterned surfaces were compatible with scanning electron microscopy (SEM) and allowed high resolution imaging of cancer cell adhesive protrusions and spreading on HA patterns to analyze cancer cell motility on exogenous HA. Use forceps to handle wafer and prevent surface defects during the entire process. 2. Transfer wafer to spin coater and cover wafer surface with SU-2025 negative photoresist. Cover at least 80% of wafer with photoresist. Spin coat for 10 seconds at 600rpm, followed by 30 seconds at 3000rpm. Due to the photosensitivity of the photoresist, turn off room lights from this point forward. 3. Transfer photoresist coated silicon wafer to hot plate for "soft-bake" at 95°C for 3 minutes. Remove from hot plate and allow 1 minute to cool. 4. Place a pre-fabricated mask with the desired pattern on top of the photoresist-covered silicon wafer...

show abstract

Hyaluronan-mediated angiogenesis in vascular disease: Uncovering RHAMM and CD44 receptor signaling pathways

Cited by 402 publications

References 97 publications

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Hyaluronan, CD44 and Emmprin: Partners in cancer cell chemoresistance

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Contact Info

Product

Resources

About