Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis

Wang, Yi; Han, Guorong; Guo, Bin; Huang, Jianhua

doi:10.1016/j.pharep.2016.07.001

Cited by 61 publications

(45 citation statements)

References 20 publications

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“…These oligosaccharides are able to interact with two critical EC receptors: CD44, playing a major role in EC adhesion and proliferation, and RHAMM (Receptor for HA-Mediated Motility), essential for EC invasion [202,203,204]. The role of LMW-HA in affecting angiogenesis during wound healing, diabetic mellitus and endometriosis, suggests the possibility to modulate HA as a putative therapeutic approach [205,206]. In contrast, high molecular weight HA (HMW-HA) was reported to be anti-angiogenic [207,208] and immunosuppressive [209].…”

Section: Hyaluronan: Not Only a Mere Gluementioning

confidence: 99%

Extracellular Matrix, a Hard Player in Angiogenesis

Mongiat

Andreuzzi

Tarticchio

et al. 2016

IJMS

172

133

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The extracellular matrix (ECM) is a complex network of proteins, glycoproteins, proteoglycans, and polysaccharides. Through multiple interactions with each other and the cell surface receptors, not only the ECM determines the physical and mechanical properties of the tissues, but also profoundly influences cell behavior and many physiological and pathological processes. One of the functions that have been extensively explored is its impingement on angiogenesis. The strong impact of the ECM in this context is both direct and indirect by virtue of its ability to interact and/or store several growth factors and cytokines. The aim of this review is to provide some examples of the complex molecular mechanisms that are elicited by these molecules in promoting or weakening the angiogenic processes. The scenario is intricate, since matrix remodeling often generates fragments displaying opposite effects compared to those exerted by the whole molecules. Thus, the balance will tilt towards angiogenesis or angiostasis depending on the relative expression of pro- or anti-angiogenetic molecules/fragments composing the matrix of a given tissue. One of the vital aspects of this field of research is that, for its endogenous nature, the ECM can be viewed as a reservoir to draw from for the development of new more efficacious therapies to treat angiogenesis-dependent pathologies.

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Section: Hyaluronan: Not Only a Mere Gluementioning

confidence: 99%

Extracellular Matrix, a Hard Player in Angiogenesis

Mongiat

Andreuzzi

Tarticchio

et al. 2016

IJMS

172

133

View full text Add to dashboard Cite

show abstract

“…Therefore, a large amount of research is currently focused on vascular regeneration to promote wound healing by enhancing angiogenesis. Many studies have reported that increasing wound angiogenesis can signi cantly promote wound repair [17][18][19][20][21]. However, because wound repair is a pathophysiological process involving multiple factors, any unbalanced factors will cause abnormal wound healing.…”

Section: Discussionmentioning

confidence: 99%

Rat epidermal stem cells promote the angiogenesis of full-thickness wound

Huang

Wang

et al. 2020

Preprint

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Abstract Background: Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds. Methods: Rat ESCs were isolated and expanded, and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division and differentiation of ESCs on the wound were analyzed by immunofluorescence. Results: The rat ESCs colonized, divided and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing. Conclusions: Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds . Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.

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“…MAPK/ERK signaling pathway plays an important role in cell proliferation, metabolism and apoptosis [ 29 ]. Initiation of Src/ERK signaling by TGF-β is important for the promotion of vascularization in the wound site [ 30 ]. SSCS showed significant effects on the expressions levels of TGF-β and CD31 in wounded tissues, especially in the early stages of wound healing, and had remarkable effects on the healing of seawater-immersed wounds, its promotional effect was superior to that of chitosan and gauze.…”

Section: Discussionmentioning

confidence: 99%

Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing

Shen

Chen

Ya³

et al. 2017

Military Med Res

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BackgroundWounded personnel who work at sea often encounter a plethora of difficulties. The most important of these difficulties is seawater immersion. Common medical dressings have little effect when the affected area is immersed in seawater, and only rarely dressings have been reported for the treatment of seawater-immersed wounds. The objective of this study is to develop a new dressing which should be suitable to prevent the wound from seawater immersion and to promote the wound healing.MethodsShark skin collagen (SSC) was purified via ethanol de-sugaring and de-pigmentation and adjusted for pH. A shark skin collagen sponge (SSCS) was prepared by freeze-drying. SSCS was attached to an anti-seawater immersion polyurethane (PU) film (SSCS + PU) to compose a new dressing. The biochemical properties of SSC and physicochemical properties of SSCS were assessed by standard methods. The effects of SSCS and SSCS + PU on the healing of seawater-immersed wounds were studied using a seawater immersion rat model. For the detection of SSCS effects on seawater-immersed wounds, 12 SD rats, with four wounds created in each rat, were divided into four groups: the 3rd day group, 5th day group, 7th day group and 12th day group. In each group, six wounds were treated with SSCS, three wounds treated with chitosan served as the positive control, and three wounds treated with gauze served as the negative control. For the detection of the SSCS + PU effects on seawater-immersed wounds, 36 SD rats were divided into three groups: the gauze (GZ) + PU group, chitosan (CS) + PU group and SSCS + PU group, with 12 rats in each group, and two wounds in each rat. The wound sizes were measured to calculate the healing rate, and histomorphology and the immunohistochemistry of the CD31 and TGF-β expression levels in the wounded tissues were measured by standard methods.ResultsThe results of Ultraviolet-visible (UV-vis) spectrum, Fourier-transform infrared (FTIR) spectrum, circular dichroism (CD) spectra, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and amino acid composition analyses of SSC demonstrated that SSC is type I collagen. SSCS had a homogeneous porous structure of approximately 200 μm, porosity rate of 83.57% ± 2.64%, water vapor transmission ratio (WVTR) of 4500 g/m2, tensile strength of 1.79 ± 0.41 N/mm, and elongation at break of 4.52% ± 0.01%. SSCS had significant beneficial effects on seawater-immersed wound healing. On the 3rd day, the healing rates in the GZ negative control, CS positive control and SSCS rats were 13.94% ± 5.50%, 29.40% ± 1.10% and 47.24% ± 8.40%, respectively. SSCS also enhanced TGF-β and CD31 expression in the initial stage of the healing period. The SSCS + PU dressing effectively protected wounds from seawater immersion for at least 4 h, and accelerated re-epithelialization, vascularization and granulation formation of seawater-immersed wounds in the earlier stages of wound healing, and as well as significantly promoted wound healing. The SSCS + PU dressing also enhanced expression of ...

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Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis

Abstract: This suggests that topical application of O-HA could be a useful method by which to treat diabetic wounds in clinical practice.

Cited by 61 publications

References 20 publications

Extracellular Matrix, a Hard Player in Angiogenesis

Extracellular Matrix, a Hard Player in Angiogenesis

Rat epidermal stem cells promote the angiogenesis of full-thickness wound

Beneficial effects of a novel shark-skin collagen dressing for the promotion of seawater immersion wound healing

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