Hyaluronic Acid and Chemoattractant Substance From Follicular Fluid: In Vitro Effect of Human Sperm Migration

Sliwa, L

doi:10.1080/014850199262751

Cited by 8 publications

(5 citation statements)

References 24 publications

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“…Follicular fluid has been analysed to look for the active substances responsible for its effects on spermatozoa. Many forms of biological substances have been found in the fluid to affect the spermatozoa similarly, such as progesterone, peptides, antisperm antibodies or immunoglobulins, and hyaluronic acid [13][14][15]. The findings from those studies signify the complicated process with many substances from endosalpinx, oocyte-cumulus complex, granulosa cell, and serum working together to direct the fertilization.…”

Section: Discussionmentioning

confidence: 94%

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Getpook

Wirotkarun

2007

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 94%

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Getpook

Wirotkarun

2007

J Assist Reprod Genet

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“…Although originally considered an inert filling material of the extracellular matrix, HA is now known to be involved in a number of biological activities, including the following: 1) fertilization (4,5), 2) embryonic development (6), 3) wound healing (7,8), 4) angiogenesis (9,10), and 5) cancer cell metastasis (11). HA may also have multiple roles in the immune response (reviewed in Ref.…”

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confidence: 99%

Hyaluronan Synthesis Is Required for IL-2-Mediated T Cell Proliferation

Mahaffey

Mummert

2007

The Journal of Immunology

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Hyaluronan (HA) is a glycosaminoglycan composed of N-acetylglucosamine and glucuronic acid subunits. Previous studies have suggested that CD44 expressed by T cells bind exogenous HA for their proliferation. However, HA endogenously synthesized by T cells may participate in their autocrine proliferation. In this study, we examined the role of endogenous HA in T cell proliferation using the highly specific HA synthase inhibitor, 4-methylumbelliferone (4-MU). We found that 4-MU inhibited the mitogen-induced synthesis of HA by T cells. Moreover, 4-MU inhibited T cell proliferation in a dose-dependent manner when cells were cultured with different stimuli, including Con A, PMA/ionomycin, and allogeneic spleen cells. Furthermore, 4-MU inhibited mitogen-stimulated IL-2 secretion, suggesting that HA may play a role in the production of this cytokine. Addition of IL-2 to T cells treated with 4-MU and Con A reversed the block in cell proliferation, showing that impaired IL-2 production is a likely mechanism for the inhibited division of T cells. Surprisingly, an anti-CD44 Ab antagonistic for HA binding did not reduce IL-2 secretion or T cell proliferation. Importantly, 4-MU did not alter the surface expression of CD44 or the ability of CD44 to bind to HA. Thus, HA-mediated IL-2 production and T cell proliferation are CD44 independent. Our results strongly suggest that HA synthesized by T cells themselves is critical for their IL-2-mediated proliferation and have revealed a previously unrecognized role for endogenous HA in T cell biology.

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“…117 Sliwa suggested that hyaluronic acid plays a part in the attraction of sperm to the ovum. 118 Hyaluronic acid in the extracellular matrix binds to cells through specific cell-surface receptors, including CD44 and the receptor for hyaluronanmediated motility (RHAMM), leading to intracellular signaling and modification of cell behavior. 119…”

Section: Biological Functionsmentioning

confidence: 99%

Final Report of the Safety Assessment of Hyaluronic Acid, Potassium Hyaluronate, and Sodium Hyaluronate

Becker¹,

Bergfeld²,

Belsito³

et al. 2009

Int J Toxicol

132

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Hyaluronic acid, sodium hyaluronate, and potassium hyaluronate function in cosmetics as skin conditioning agents at concentrations up to 2%. Hyaluronic acid, primarily obtained from bacterial fermentation and rooster combs, does penetrate to the dermis. Hyaluronic acid was not toxic in a wide range of acute animal toxicity studies, over several species and with different exposure routes. Hyaluronic acid was not immunogenic, nor was it a sensitizer in animal studies. Hyaluronic acid was not a reproductive or developmental toxicant. Hyaluronic acid was not genotoxic. Hyaluronic acid likely does not play a causal role in cancer metastasis; rather, increased expression of hyaluronic acid genes may be a consequence of metastatic growth. Widespread clinical use of hyaluronic acid, primarily by injection, has been free of significant adverse reactions. Hyaluronic acid and its sodium and potassium salts are considered safe for use in cosmetics as described in the safety assessment.

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Hyaluronic Acid and Chemoattractant Substance From Follicular Fluid: In Vitro Effect of Human Sperm Migration

Cited by 8 publications

References 24 publications

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Sperm motility stimulation and preservation with various concentrations of follicular fluid

Hyaluronan Synthesis Is Required for IL-2-Mediated T Cell Proliferation

Final Report of the Safety Assessment of Hyaluronic Acid, Potassium Hyaluronate, and Sodium Hyaluronate

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