Hyaluronic Acid and Polyethylene Glycol Hybrid Hydrogel Encapsulating Nanogel with Hemostasis and Sustainable Antibacterial Property for Wound Healing

Abstract

Immediate hemorrhage control and anti-infection play important roles in the wound management. Besides, a moist environment is also beneficial for wound healing. Hydrogels are promising materials in urgent hemostasis and drug release. However, hydrogels have the disadvantage of rapid release profiles, leading to the exposure to high drug concentrations. In this study, we constructed hybrid hydrogels with rapid hemostasis and sustainable antibacterial property combining aminoethyl methacrylate hyaluronic acid (HA-AEMA) and methacrylated methoxy polyethylene glycol (mPEG-MA) hybrid hydrogels and chlorhexidine diacetate (CHX)-loaded nanogels. The CHX-loaded nanogels (CLNs) were prepared by the enzyme degradation of CHX-loaded lysine-based hydrogels. The HA-AEMA and mPEG-MA hybrid hydrogel loaded with CLNs (labeled as Gel@CLN) displayed a three-dimensional microporous structure and exhibited excellent swelling, mechanical property, and low cytotoxicity. The Gel@CLN hydrogel showed a prolonged release period of CHX over 240 h and the antibacterial property over 10 days. The hemostasis and wound-healing properties were evaluated in vivo using a mouse model. The results showed that hydrogel had the rapid hemostasis capacity and accelerated wound healing. In summary, CLN-loaded hydrogels may be excellent candidates as hemostasis and anti-infection materials for the wound dressing application.

Biodegradable Antibacterial Polymeric Nanosystems: A New Hope to Cope with Multidrug‐Resistant Bacteria

“…In recent years, the development of multifunctional antibacterial materials with hemostasis and wound‐healing capabilities has attracted increasing attention in the field of biomaterials . The chlorhexidine diacetate (CHX)‐loaded nanogels (CLN) were first prepared via enzyme degradation procedure, and the obtained CLNs were mixed with precursors of aminoethyl methacrylate hyaluronic acid (HA‐AEMA) and methacrylated methoxy polyethylene glycol (mPEG‐MA) for photoinduced cross‐linking ( Figure a) . Then the formation of CLN‐loaded hybrid hydrogel could be applied for the combination of bacterial eradication, bleeding control, and enhanced wound healing.…”

“…b) Wound healing processes after treated with different materials. Reproduced with permission . Copyright 2018, American Chemical Society.…”

Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing

“…The majority of hydrogels are prepared from natural polymer materials (e.g., alginate, carboxymethylcellulose, dextran, gelatin, collagen, and hyaluronic acid) and synthetic polymer materials [e.g., methoxy polyethylene glycol, poly(vinyl alcohol), peptide, and polyamidoamine] because of their excellent biocompatibility and biodegradability (Travan et al, 2016). Hyaluronic acid (HA), the main component of the extracellular membrane (ECM), can increase cell-matrix interaction and initiate the signal transduction essential for cell survival and function and has been widely used in the biomedical materials field because of its property of easily peeling, excellent biocompatibility, and high water retention (Purcell et al, 2012;Julia et al, 2013;Zhu et al, 2018a;Liang et al, 2019b).…”

Mussel‐Inspired Adhesive Polydopamine‐Functionalized Hyaluronic Acid Hydrogel with Potential Bacterial Inhibition

“…[1] Thermosensitive chitosan-based hydrogel could encapsulate exogenous recombinant human stromal cell-derived factor-1 alpha to recruit mesenchymal stem cells for corneal epithelium regeneration. [4][5][6][7][8] Especially, these hydrogel-based dressings present various properties such as absorbing exudates from wounds, in situ encapsulating drugs and filling irregular wound sites, Global Challenges 2020, 4,1900068 HA with cystamine dihydrochloride (CD) and disulfide bond cleavage. [3] In recent years, natural polymer hydrogels have been demonstrated with the potential as wound dressings because they can afford a moist environment to promote the wound healing.…”

“…[3] In recent years, natural polymer hydrogels have been demonstrated with the potential as wound dressings because they can afford a moist environment to promote the wound healing. [4][5][6][7][8] Especially, these hydrogel-based dressings present various properties such as absorbing exudates from wounds, in situ encapsulating drugs and filling irregular wound sites, Global Challenges 2020, 4,1900068 HA with cystamine dihydrochloride (CD) and disulfide bond cleavage. The successful amidation of HA was verified by Fourier transform infrared (FT-IR) and 1 H NMR.…”