Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro

Moser, Lukas B.; Bauer, Christoph; Jeyakumar, Vivek; Niculescu-Morzsa, Eugenia-Paulina; Nehrer, Stefan

doi:10.3390/ijms222111503

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…A number of studies have noted significantly higher cell viability in cultured human chondrocytes after exposure to ropivacaine 1%, similar to control levels, than what was seen after exposure to either bupivacaine 0.25% or lidocaine 1% for 1 h. 57 Shaw et al 58 compared the chondrotoxicity of bupivacaine (0.5%), ropivacaine (0.5%), and 1.3% liposomal bupivacaine on bovine chondrocyte derived cells. Cells were plated and exposed to each solution without dilution for 1 h, then were washed and allowed to incubate in medium for an additional 23 h. Liposomal bupivacaine had the highest cell viability (as assessed by flow cytometry) of all treatment groups, followed by ropivacaine and bupivacaine, which had the lowest chondrocyte viability of the anaesthetic solutions tested.…”

Section: In Vitro Studies Of Toxicitymentioning

confidence: 88%

“…Cell density and synoviocyte viability were further reduced ex vivo in the methylprednisolone/lidocaine group at day seven, leading the authors to conclude there is potential for cytotoxicity to both chondrocytes and synoviocytes treated with a corticosteroid/LA combination; however, the individual contributions of LA versus steroids was not investigated. Moser et al 57 investigated in vitro the effects of various LA (lidocaine, bupivacaine, ropivacaine) combined with glucocorticoids (GC), hyaluronic acid (HA) or both using cultured human chondrocytes. When examined under a microscope, chondrocytes had increased branching and enhanced attachment when exposed to HA and GC/HA compared to local anaesthetics alone or local anaesthetics with glucocorticoids.…”

Section: Additives To Local Anaesthetic Injectionsmentioning

confidence: 99%

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Review of intra‐articular local anaesthetic administration in horses: Clinical indications, cytotoxicity, and outcomes

Webster,

Pezzanite,

Hendrickson

et al. 2023

Equine Veterinary Journal

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Equine practitioners frequently inject local anaesthetics (LA) intra‐articularly in both diagnosis of lameness and for pain management intra‐ or post‐operatively with synovial endoscopy. Recent reviews of the human and veterinary literature support the concept that chondrotoxicity of LA on joint tissues depends on the type of drug, dose administered, and duration of exposure. The purpose of this review is to summarise the current literature describing intra‐articular local anaesthetic use, including both in vitro and in vivo studies, and to draw some comparisons to literature from other species where potential toxicity and duration of effect have been evaluated with the goal of advancing the field's understanding of intra‐articular local anaesthetic use in horses, and indicating future directions for the field. The aggregate data available from all species, while generally sparse for horses, indicate that LA are rapidly cleared from the synovial fluid after injection, often within 30 min. In vitro data strongly suggest that lidocaine and bupivacaine are likely more chondrotoxic than other LA, although to what extent is still unknown, and cytotoxicity of LA may be mitigated through concurrent injection with HA, PRP, and drug combinations including nonsteroidal anti‐inflammatories and opioids. The current body of in vitro research is not reflective of the in vivo environment, and further in vitro work, if performed, should focus on mimicking the native joint environment, utilising PK data and joint/injection volumes to replicate the native environment more accurately within the joint and the expected exposures to LA.

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Section: In Vitro Studies Of Toxicitymentioning

confidence: 88%

Section: Additives To Local Anaesthetic Injectionsmentioning

confidence: 99%

Review of intra‐articular local anaesthetic administration in horses: Clinical indications, cytotoxicity, and outcomes

Webster,

Pezzanite,

Hendrickson

et al. 2023

Equine Veterinary Journal

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“…[32] Therapeutic trajectory following intra-articular hyaluronic acid injection in KOA - meta-analysis yielded the same conclusion, namely that IAHA is efficacious at 4 weeks, reaches maximum efficacy at 8 weeks, and exerts detectable residual effects at 24 weeks. [33] In addition, a number of studies [34] have investigated whether HA can attenuate the cytotoxic effects of glucocorticoids and anesthetics. One study examined the efficacy and safety of Diclofenac-Hyaluronate (DF-HA) Conjugate (Diclofenac Etalhyalu-rate) in the treatment of KOA utilizing DF covalently linked to a high molecular weight fermented HA.…”

Section: Discussionmentioning

confidence: 99%

A bibliometric analysis of intra-articular injection therapy for knee osteoarthritis from 2012 to 2022

Lu,

Xie,

Liu

et al. 2023

Medicine

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Knee osteoarthritis (KOA) is the most common joint disease worldwide and, with the progression of an aging population, is one of the most important causes of disability worldwide. Its main symptoms include articular cartilage damage, periarticular pain, swelling, and stiffness. Intra-articular (IA) injections offer many advantages over systemic administration and surgical treatment, including direct action on the target joint to improve local bioavailability, reduce systemic toxicity, and lower costs. This study analyzed KOA intra-articular injection treatment and its hot literature and research horizons using bibliometric methodologies and graphical tools to aid future research. We performed a bibliometric analysis of 2360 publications in the Web of Science core collection using CiteSpace software. The United States (28.26% of publications) and China (18%) had the biggest publications. Rush University was the most active institution, but Boston University had the greatest citation/publication rate (65.77), suggesting a high literature standard. The majority of publications were in Osteoarthritis and cartilage. Bannuru RR was the most referenced author, while Filardo, Giuseppe was the most productive author. Studies in platelet-rich plasma (PRP), mesenchymal stem cells (MSCs), and microsphere formulation are likely to be future research hotspots. The current scientometric study provides an overview of KOA intra-articular injection therapy studies from 2012 to 2022. This study outlines the current research hotspots and potential future research hotspots in the field of intra-articular injection treatment for KOA and may serve as a resource for researchers interested in this topic.

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“…Cell isolation from shavered or scalpel minced cartilage fragments was performed overnight via enzymatic release using Liberase™ (0.2 WU/mL, Roche Diagnostics GmbH, Mannheim, Germany) in a shaker at 30 rpm at 37 °C, as previously described [ 17 ]. On the second day, 1*10 5 cells were resuspended in 100 µl annexin staining buffer (10 mM CaCl 2 , HEPES) and stained with 5 µl PE-labeled annexin V and 1 µl 7-Aminoactinomycin (7-AAD) from an apoptosis detection kit (BD Pharmingen, #559793).…”

Section: Methodsmentioning

confidence: 99%

Mincing bovine articular cartilage with commercially available shavers reduces the viability of chondrocytes compared to scalpel mincing

Moser,

Bauer,

Otahal

et al. 2023

J. exp. orthop.

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Purpose The study aimed to compare the effect of mincing bovine articular cartilage with different shaver blades on chondrocyte viability. Methods Bovine articular cartilage was harvested either with a scalpel or with three different shaver blades (2.5 mm, 3.5 mm, or 4.2 mm) from a commercially available shaver. The cartilage harvested with a scalpel was then minced into fragments smaller than 1 mm3 with a scalpel. All four conditions were cultivated in a culture medium for seven days. After Day 1 and Day 7, the following measurements were performed: metabolic activity, RNA isolation, and gene expression of anabolic (COL2A1 and ACAN) and catabolic genes (MMP1 and MMP13), live/dead staining and visualization using confocal microscopy, and flow cytometric characterization of minced cartilage chondrocytes. Results Mincing the cartilage with shavers significantly reduced metabolic activity after one and seven days compared to scalpel mincing (p < 0.001). Gene expression of anabolic genes (COL2A1 and ACAN) was reduced, while catabolic genes (MMP1 and MMP13) were increased after day 7 in all shaver conditions. Confocal microscopy showed a thin line of dead cells at the lesion side with viable cells beneath for the scalpel mincing and a higher number of dead cells diffusely distributed in the shaver conditions. After seven days, there was a significant decrease in viable cells in the shaver conditions compared to scalpel mincing (p < 0.05). Flow cytometric characterization revealed fewer intact cells and proportionally more dead cells in all shaver conditions compared to the scalpel mincing. Conclusion Mincing bovine articular cartilage with commercially available shavers reduces the viability of chondrocytes compared to scalpel mincing immediately after harvest and after seven days in culture. This suggests that mincing cartilage with a shaver should be considered a matrix rather than a cell therapy. Level of evidence Level II therapeutic study.

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Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro

Cited by 7 publications

References 35 publications

Review of intra‐articular local anaesthetic administration in horses: Clinical indications, cytotoxicity, and outcomes

Review of intra‐articular local anaesthetic administration in horses: Clinical indications, cytotoxicity, and outcomes

A bibliometric analysis of intra-articular injection therapy for knee osteoarthritis from 2012 to 2022

Mincing bovine articular cartilage with commercially available shavers reduces the viability of chondrocytes compared to scalpel mincing

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