Hyaluronic acid biodegradable material for reconstruction of vascular wall: A preliminary study in rats

Pandis, Laura; Zavan, Barbara; Bassetto, Franco; Ferroni, Letizia; Iacobellis, Laura; Abatangelo, Giovanni; Lepidi, Sandro; Cortivo, Roberta; Vindigni, Vincenzo

doi:10.1002/micr.20856

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…In situ tissue engineering has demonstrated significant advances in development of artificial blood vessel shunts as well as CEA vascular patches. Biodegradable patches made from various biologically active compounds have previously demonstrated a partial restoration of vascular wall at the implantation site. − One of the aims of this study was to evaluate the in situ regeneration potential of our experimental tissue-engineered patches. The formation of the endothelial layer on TTDDA-modified patches was faster than that on unmodified PHBV/PCL samples.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

et al. 2020

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Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated the performance of vascular patches electrospun using a blend of polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV) and additionally modified with RGDK, AhRGD, and c[RGDFK] peptides using 1,6-hexamethylenediamine or 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) linkers. We examined mechanical properties and hemocompatibility of resulting patches before implanting them in rat abdominal aortas to assess their performance in vivo. Patches were explanted 1, 3, 6, and 12 months postoperation followed by histological and immunofluorescence analyses. Patches manufactured from the human internal mammary artery or commercially available KemPeriplas-Neo xenopericardial patches were used as a control. The tensile strength and F max of KemPeriplas-Neo patches were 4- and 16.7-times higher than those made of human internal mammary artery, respectively. Both RGD-modified and unmodified PHBV/PCL patches demonstrated properties similar to a human internal mammary artery patch. Regardless of RGD modification, experimental PHBV/PCL patches displayed fewer lysed red blood cells and resulted in milder platelet aggregation than KemPeriplas-Neo patches. Xenopericardial patches failed to form an endothelial layer in vivo and were prone to calcification. By contrast, TTDDA/RGDK-modified biodegradable patches demonstrated a resistance to calcification. Modification by TTDDA/RGDK and TTDDA/c[RGDFK] facilitated the formation of neovasculature upon the implantation in vivo.

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Section: Discussionmentioning

confidence: 99%

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

et al. 2020

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“…Considering the above-mentioned data, HA was investigated for its role in inducing complete vascular regeneration directly in vivo by the formation of the vascular conduit. In particular, Hyaff-11 ® biomaterial was utilized to obtain tubules of 2 mm in diameter (50 μm thick) to be used as a vascular prosthesis in animal experimental models [ 93 , 94 ] A segment of rat aorta (2 cm) was incised and the Hyaff tubule, 2 mm in diameter and 2 cm in length, was anastomized, first proximally, then distally in an end-to-end fashion [ 95 ]. Similar experiments were performed on the pig carotid artery and on the rat vena cava [ 96 , 97 ].…”

Section: Medical Applications Of Ha and Its Derivativesmentioning

confidence: 99%

Hyaluronic Acid: Redefining Its Role

Abatangelo

Vindigni

Avruscio

et al. 2020

Cells

316

166

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The discovery of several unexpected complex biological roles of hyaluronic acid (HA) has promoted new research impetus for biologists and, the clinical interest in several fields of medicine, such as ophthalmology, articular pathologies, cutaneous repair, skin remodeling, vascular prosthesis, adipose tissue engineering, nerve reconstruction and cancer therapy. In addition, the great potential of HA in medicine has stimulated the interest of pharmaceutical companies which, by means of new technologies can produce HA and several new derivatives in order to increase both the residence time in a variety of human tissues and the anti-inflammatory properties. Minor chemical modifications of the molecule, such as the esterification with benzyl alcohol (Hyaff-11® biomaterials), have made possible the production of water-insoluble polymers that have been manufactured in various forms: membranes, gauzes, nonwoven meshes, gels, tubes. All these biomaterials are used as wound-covering, anti-adhesive devices and as scaffolds for tissue engineering, such as epidermis, dermis, micro-vascularized skin, cartilage and bone. In this review, the essential biological functions of HA and the applications of its derivatives for pharmaceutical and tissue regeneration purposes are reviewed.

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“…Этот подход также применяют при разработке новых сосудистых заплат. Частичное восстановление сосудистой стенки на месте имплантации биодеградируемой заплаты показано в работах с имплантатами из бензилового эфира гиалуроновой кислоты, на основе тканого слоя PLA, окруженного с двух сторон слоями пористого спонжа из сополимера PCL и PLA, а также из шелка тутового шелкопряда, обработанного 4-гексилрезорцином [28][29][30]. В настоящей работе изучение способности разрабатываемых заплат поддерживать регенерацию сосудистой стенки in situ также стало одной из главных задач.…”

Section: Discussionunclassified

Tissue-engineered patch modified by vascular endothelial growth factor for reconstruction of vascular wall

Sevost'yanova¹,

Миронов²,

Антонова³

et al. 2020

PKiK

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Background. Commercially available synthetic and animal-derived vascular patches used in patch angioplasty during carotid endarterectomy have several disadvantages, such as postoperative thrombosis or occlusion and restenosis. This problem may be resolved by the development of biologically active materials that are biodegradable and can stimulate tissue regeneration. Aim. To evaluate the properties and efficacy of a biodegradable patch based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) into which vascular endothelial growth factor (VEGF) is incorporated, in comparison with unmodified PHBV/PCL and commercial vascular patches.Methods. Porous patches were fabricated by emulsion electrospinning from a mixture of PHBV and PCL, into which VEGF was incorporated. The morphological and mechanical properties of these patches were tested, and they were implanted into the wall of rat abdominal aortas for 1, 3, 6 and 12 months. Histological and immunofluorescence examinations were performed to evaluate endothelisation, cellular composition and calcification.Results. PHBV / PCL patches with VEGF had a highly porous structure and demonstrated tensile strength similar to that of the aorta in rats and the internal thoracic artery in humans. After 3 months of implantation, an endothelial monolayer was formed on the inner surface of these patches. The patches were populated by cells that secreted the extracellular matrix faster than did cells of patches from the xenopericardium. Remodelling with PHBV / PCL patches was not accompanied by chronic inflammation; in contrast, inflammation was observed with long-term implantation of unmodified PHBV / PCL samples.Conclusion. VEGF incorporated into biodegradable PHBV / PCL patches stimulated their endothelisation, increased their biocompatibility and promoted remodelling and formation of the components of the blood vessel. PHBV / PCL / VEGF patches thus have a high potential for use in tissue engineering of the vascular wall.Received 2 June 2020. Revised 27 June 2020. Accepted 16 July 2020.Funding: This study was supported by the Complex Program of Basic Research under the Siberian Branch of the Russian Academy of Sciences within the Basic Research Topic of Research Institute for Complex Issues of Cardiovascular Diseases № 0546-2019-0002 “Pathogenetic basis for the development of cardiovascular implants from biocompatible materials using patient-oriented approach, mathematical modeling, tissue engineering, and genomic predictors”.Conflict of interest: Authors declare no conflict of interest.Author contributions Conception and study design: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, R.S. Tarasov, L.S. Barbarash Data collection and analysis: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, E.O. Krivkina, V.G. Matveeva, E.A. Velikanova, T.V. Glushkova Statistical analysis: V.V. Sevostianova, T.V. Glushkova Drafting the article: V.V. Sevostianova, A.V. Mironov Critical revision of the article: L.V. Antonova, R.S. Tarasov, L.S. Barbarash Final approval of the version to be published: V.V. Sevostianova, A.V. Mironov, L.V. Antonova, E.O. Krivkina, V.G. Matveeva, E.A. Velikanova, R.S. Tarasov, T.V. Glushkova, L.S. Barbarash

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Hyaluronic acid biodegradable material for reconstruction of vascular wall: A preliminary study in rats

Cited by 11 publications

References 51 publications

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study

Hyaluronic Acid: Redefining Its Role

Tissue-engineered patch modified by vascular endothelial growth factor for reconstruction of vascular wall

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