Hyaluronic Acid Can be a New Plant Growth Regulator for Hybrid Cymbidium Micropropagation

Abstract

Present authors investigated the effect of two biopolymer hyaluronic acid (HA9) and chitosan (Chitosan H), and N 6 -benzyladenine (BA) supplemented with modified MS on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium cultivar. The percentages of PLB formation from cultures showed 100 % in all treatments except for control (82.7%), 0.1 mg/l HA9 (93.3%) and 10 mg/l Chitosan H (93.3%) treatments. Addition of 0.1 mg/l HA9 to culture medium was highly efficient for PLB multiplication. Both 10 mg/l HA9 and 1.0 mg/l BA significantly increased the number of shoots in cultures. The maximum percentage of shoot (100%) was obtained at 1.0 mg/l chitosan H. Increase in fresh weight showed higher values at BA treatments. Information gained from this study shows that hyaluronic acid (HA9) which is new additive for plant tissue culture act as growth regulator for hybrid Cymbidium micropropagation.

Effect of the Elicitor Hyaluronic acid on Multiplication and Indirect Regeneration of Curcuma longa L. Plant

“…In this concern, Habiba et al (2014) found that HA at 1 mg l -1 produced the highest shoots per explant of Cymbidium dayanum and Dendrobium kingianum respectively. Whereas, Kaewjampa et al (2012) found that 0.1 mg l -1 HA on culture medium was highly potent in PLB (protocorm like bodies)multiplication. Results of Haque et al(2016) showed that BA at 0.1 mg l -1 combined with HA at 0.1 mg l -1 are the most effective concentration for PLB, shoot induction and maximum fresh weight of C.insigne.…”

“…In general, such this work on turmeric was not done before according to the available researchand previous studies were on the Orchid Cymbidium only. Another successful result has been obtained by Kaewjampa et al(2012)and Nahar et al(2011). They investigated the effect of two biopolymer HA and Chitosan H, and BA supplemented with modified MS on organogenesis in protocorm like bodies (PLBs) of a Cymbidium.…”

“…HA composed of glucuronic acid and N-acetyl glucosamine joined alternately by glycosidic bonds, has biological functions in lower and higher organisms, including in cell adhesion, migration, proliferation and differentiation, regulation of protein secretion, and gene expression (Kogan et al, 2007). HA suppresses disease in cucumber, tomato and pepper (Park et al, 2008) also been shown to improve orchid (Cymbidium dayanum) organogenesis in vitro (Nahar et al, 2011;Kaewjampa et al, 2012).…”

PENGARUH KONSENTRASI ROOTONE-F DAN PANJANG SETEK PADA PERTUMBUHAN Rhododendron mucronatum G. Don. var. phoeniceum

“…phoeniceum. Kandungan bahan aktif berupa IAA, IBA, dan NAA bekerja pada jaringan meristem akar sehingga membentuk sistem perakaran baru (Kaewjampa, et al 2013) (Santoso, et al 2008). Panjang setek yang baik untuk masing-masing jenis tanaman berbeda satu dengan yang lainnya.…”

Effect of light quality and plant growth regulator on organogenesis of orkid Cymbidium dayanum

“…HA suppresses disease in cucumber, tomato and pepper (Park et al, 2008) and has shown to have antioxidant activity. HA have of recent been suggested as important components of Cymbidium in vitro culture media (Nahar et al, 2011Nahar and Shimasaki, 2014;Kaewjampa et al, 2012). Therefore, the objective of this study was to investigate the effect of light quality and plant growth regulator on organogenesis of C. dayanum in vitro.…”