Hyaluronic acid chloramide—Synthesis, chemical structure, stability and analysis of antimicrobials

Buffa, Radovan; Hermannová, Martina; Sojka, Martin; Svozil, Vít; Šulc, Petr; Halamková, Pavlína; Pospíšilová, Michaela; Krejčí, Helena; Velebný, Vladimı́r

doi:10.1016/j.carbpol.2020.116928

Cited by 12 publications

(6 citation statements)

References 19 publications

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“…H 2 O 2 or HOCl solutions were freshly prepared by diluting commercially available H 2 O 2 (Cat #216763, Sigma-Aldrich, St. Louis, MO, USA) and NaOCl (Cat #425044, Sigma-Aldrich; available as chlorine 10–15%) in degassed autoclaved deionized water. Prior to the oxidation reaction, the HA solution was prepared and left under gentle agitation for 24 h to allow for full solvation [ 57 ]. At pH of 7.4, the dissociation of NaOCl (pK a = 7.53) leads to a nearly equimolar concentration of HOCl and ClO - ions, which we hereafter refer to as HOCl.…”

Section: Methodsmentioning

confidence: 99%

“…Thereafter, the effects of oxHA and size-matched native HA were evaluated on corneal wound healing in vitro by assaying cell behaviors associated with wound healing, namely, viability, proliferation, and migration, as well as in vivo using corneal debridement wounds in mice. Prior to the oxidation reaction, the HA solution was prepared and left under gentle agitation for 24 h to allow for full solvation [57]. At pH of 7.4, the dissociation of NaOCl (pK a = 7.53) leads to a nearly equimolar concentration of HOCl and ClOions, which we hereafter refer to as HOCl.…”

Section: Introductionmentioning

confidence: 99%

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ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

Lin,

Moreno,

Nguyen

et al. 2023

Biomolecules

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A buildup of reactive oxygen species (ROS) occurs in virtually all pathological conditions. Hyaluronan (HA) is a major extracellular matrix component and is susceptible to oxidation by reactive oxygen species (ROS), yet the precise chemical structures of oxidized HA products (oxHA) and their physiological properties remain largely unknown. This study characterized the molecular weight (MW), structures, and physiological properties of oxHA. For this, high-molecular-weight HA (HMWHA) was oxidized using increasing molar ratios of hydrogen peroxide (H2O2) or hypochlorous acid (HOCl). ROS lead to the fragmentation of HA, with the oxHA products produced by HOCl exhibiting an altered chemical structure while those produced by H2O2 do not. HMWHA promotes the viability of human corneal epithelial cells (hTCEpi), while low MWHA (LMWHA), ultra-LMWHA (ULMWHA), and most forms of oxHA do not. HMWHA and LMWHA promote hTCEpi proliferation, while ULMWHA and all forms of oxHA do not. LMWHA and some forms of oxHA promote hTCEpi migration, while HMWHA does not. Finally, all native forms of HA and oxHA produced by HOCl promote in vivo corneal wound healing, while oxHA produced by H2O2 does not. Taken together, our results show that HA fragmentation by ROS can alter the physiological activity of HA by altering its MW and structure.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

Lin,

Moreno,

Nguyen

et al. 2023

Biomolecules

View full text Add to dashboard Cite

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“…Through preliminary tests, the HA derivative could reduce the growth of bacteria, which would be potential material in the application of biomedicine. 198 2.4. Cationic Antimicrobial Peptidopolysaccharides.…”

Section: Cationic Peptides and Macromolecules Asmentioning

confidence: 99%

“…In addition to the above-mentioned polysaccharides, other kinds of polysaccharides, such as pullulan, hyaluronic acid, alginic acid, and fucoidan, have also been modified for antimicrobial purposes. ,− Antimicrobial pullulan derivative modified by the grafting of 3-aminopropyltrimethoxysilane was reported. Compared with pure pullulan film, the modified pullulan derivative film had 4–5 log reductions against S. aureus and E. coli .…”

Section: Cationic Peptides and Macromolecules As Antimicrobial Agentsmentioning

confidence: 99%

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Antimicrobial Peptides and Macromolecules for Combating Microbial Infections: From Agents to Interfaces

Zhang

et al. 2022

ACS Appl. Bio Mater.

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Bacterial resistance caused by the overuse of antibiotics and the shelter of biofilms has evolved into a global health crisis, which drives researchers to continuously explore antimicrobial molecules and strategies to fight against drug-resistant bacteria and biofilm-associated infections. Cationic antimicrobial peptides (AMPs) are considered to be a category of potential alternative for antibiotics owing to their excellent bactericidal potency and lesser likelihood of inducing drug resistance through their distinctive antimicrobial mechanisms. In this review, the hitherto reported plentiful action modes of AMPs are systematically classified into 15 types and three categories (membrane destructive, nondestructive membrane disturbance, and intracellular targeting mechanisms). Besides natural AMPs, cationic polypeptides, synthetic polymers, and biopolymers enable to achieve tunable antimicrobial properties by optimizing their structures. Subsequently, the applications of these cationic antimicrobial agents at the biointerface as contact-active surface coatings and multifunctional wound dressings are also emphasized here. At last, we provide our perspectives on the development of clinically significant cationic antimicrobials and related challenges in the translation of these materials.

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A natural antibacterial agent based on modified chitosan by hinokitiol for antibacterial application on cotton fabric

et al. 2022

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Hyaluronic acid chloramide—Synthesis, chemical structure, stability and analysis of antimicrobials

Cited by 12 publications

References 19 publications

ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

ROS-Mediated Fragmentation Alters the Effects of Hyaluronan on Corneal Epithelial Wound Healing

Antimicrobial Peptides and Macromolecules for Combating Microbial Infections: From Agents to Interfaces

A natural antibacterial agent based on modified chitosan by hinokitiol for antibacterial application on cotton fabric

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