Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Chen, Zhipeng; Chen, Juan; Wu, Li; Li, Weidong; Chen, Jun; Cheng, Haibo; Jin-huo, Pan; Cai, Baochang

doi:10.2147/ijn.s50721

Cited by 61 publications

(38 citation statements)

References 19 publications

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“…Previous experiments have shown that an antibody directed against CD44 competes with HA-NPs for binding to receptors expressed on cell membranes [20]. As demonstrated in cancer cells [4] and in previous work for chondrocytes targeting [17], HA / CD44 recognition plays an active role in the internalization process in articular cells. In previous experiment, we have confirmed that internalization of cyanine-3 labelled, HA covered NPs was almost totally suppressed after CD44 blockade with a specific antibody.…”

Section: Discussionmentioning

confidence: 71%

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PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies

et al. 2015

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Section: Discussionmentioning

confidence: 71%

“…HA is a major component of extracellular matrix and synovial fluid and is internalized by chondrocytes through CD44 binding [16]. HA-covered active particles represent therefore a novel and attractive therapeutic approach in the joint [17].…”

Section: Introductionmentioning

confidence: 99%

PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies

et al. 2015

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“…Therefore, this model is suitable for determining whether an acute inflammatory response could be prevented.The NPs loaded with both salmon calcitonin and HA successfully reduced joint inflammation, preserved bone architecture, and decreased inflammatory gene expression, highlighting the powerful anti-inflammatory effects of these drugs with NP delivery. A similar in vivo NP study showed that HA increased chondrocyte targeting and retention time of NPs in healthy rat knees 72 , suggesting that HA can potentially be used both for targeting and therapy, similar to CS. Future work with HA-loaded NPs should focus on its efficacy in treating and/or targeting OA-affected joints using a validated OA animal model.…”

Section: Drugs That Inhibit Inflammationmentioning

confidence: 84%

“…For example, Brucine is an anti-apoptotic alkaloid. Brucine may aid in cartilage regeneration by inhibiting apoptosis and acting as an antagonist to nitric oxide, an inhibitor of chondrocyte proliferation 72 . However, brucine is severely toxic to the central nervous system and, therefore, requires local delivery to the joint.…”

Section: Drugs That Protect Chondrocytesmentioning

confidence: 99%

Osteoarthritis: Pathology, Mouse Models, and Nanoparticle Injectable Systems for Targeted Treatment

et al. 2016

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Osteoarthritis (OA) is a progressive, degenerative disease of articulating joints that not only affects the elderly, but also involves younger, more active individuals with prolonged participation in high physical-demand activities. Thus, effective therapies that are easy to adopt clinically are critical in limiting the societal burden associated with OA. This review is focused on intra-articular injectable regimens and provides a comprehensive look at existing in vivo models of OA that might be suitable for developing, testing, and finding a cure for OA by intra-articular injections. We first discuss the pathology, molecular mechanisms responsible for the initiation and progression of OA, and challenges associated with disease-specific targeting of OA. We proceed to discuss available animal models of OA and provide a detailed perspective on the use of mouse models in studies of experimental OA. We finally provide a closer look at intra-articular injectable treatments for OA, focusing on biomaterials-based nanoparticles, and provide a comprehensive overview of the various nanometer-size ranges studied.

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“…To further reveal the uptake pathways of BSA-V-NPs, BGC-823 cells were preincubated with the endocytic inhibitors sucrose (450 mM, inhibiting clathrinmediated endocytosis) and nystatin (25 μg/mL inhibiting caveolae-mediated endocytosis), respectively. 21,27 After removing the inhibitor solution, the cells were washed thrice and further treated with DOX-BSA-V-NPs for 4 hours. Finally, the cells were washed, fixed, and observed using an inverted fluorescence microscope (Olympus).…”

Section: Cellular Uptake and Uptake Mechanismmentioning

confidence: 99%

Enhanced tumor delivery and antitumor response of doxorubicin loaded albumin nanoparticles formulated based on a Schiff base

Li¹,

Zheng²,

Xin³

et al. 2016

IJN

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A novel method was developed here to prepare albumin-based nanoparticles (NPs) for improving the therapeutic and safety profiles of chemotherapeutic agents. This approach involved crosslinking bovine serum albumin (BSA) using a Schiff base-containing vanillin, into NPs and loading doxorubicin (DOX) into the NPs by incubation. The resultant NPs (DOX-BSA-V-NPs) displayed a particle size of 100.5±1.3 nm with a zeta potential of −23.05±1.45 mV and also showed high drug-loading efficiency and excellent stability with respect to storage and temperature. The encapsulation of DOX into the BSA-V-NPs was confirmed by dynamic scanning calorimetry and Raman spectroscopy. DOX-BSA-V-NPs exhibited a significantly faster DOX release at pH 6.5 than pH 7.4, as well as in a solution with a higher glutathione concentration. In vitro studies showed that the cellular uptake of DOX-BSA-V-NPs was time-dependent, concentration-dependent, and faster than free DOX, while the cytotoxicity of DOX-BSA-V-NPs (IC 50 value of 3.693 μg/mL) was superior to free DOX (IC 50 value of 4.007 μg/mL). More importantly, DOX-BSA-V-NPs showed a longer mean survival time of 24.83 days, a higher tumor inhibition rate of 56.66%, and a decreased distribution in the heart than other DOX formulations in animal studies using a tumor xenograft model. Thus, the vanillin-based albumin NPs were shown here to be a promising carrier for tumor-targeted delivery of chemotherapeutic agents and, thus, should be further studied.

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Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Cited by 61 publications

References 19 publications

PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies

PLGA-Based Nanoparticles: a Safe and Suitable Delivery Platform for Osteoarticular Pathologies

Osteoarthritis: Pathology, Mouse Models, and Nanoparticle Injectable Systems for Targeted Treatment

Enhanced tumor delivery and antitumor response of doxorubicin loaded albumin nanoparticles formulated based on a Schiff base

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