Hyaluronic acid detection and relative quantification by mass spectrometry imaging in human skin tissues

Legouffe, Raphael; Jeanneton, Olivier; Gaudin, Mathieu; Tomezyk, Aurore; Gerstenberg, Amandine; Dumas, Marc; Heusèle, Catherine; Bonnel, David; Stauber, Jonathan; Schnebert, Sylvianne

doi:10.1007/s00216-022-04139-8

Cited by 17 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study we showed that the HA content was significantly lower in the IMCT of elderly human subjects and mice studied, as already demonstrated in the skin as a physiological process influenced by aging [ 34 , 35 , 36 ]. This HA reduction with aging, along with the accumulation of collagen content and the reduction in elastic fibers inhibits the tissue from absorbing water and contributes to become much more rigid and less elastic, with altered gliding properties.…”

Section: Discussionsupporting

confidence: 79%

The Effects of Aging on the Intramuscular Connective Tissue

Fede

Fan

Pirri

et al. 2022

IJMS

View full text Add to dashboard Cite

The intramuscular connective tissue plays a critical role in maintaining the structural integrity of the muscle and in providing mechanical support. The current study investigates age-related changes that may contribute to passive stiffness and functional impairment of skeletal muscles. Variations in the extracellular matrix in human quadriceps femoris muscles in 10 young men, 12 elderly males and 16 elderly females, and in the hindlimb muscles of 6 week old, 8 month old and 2 year old C57BL/6J male mice, were evaluated. Picrosirius red, Alcian blue and Weigert Van Gieson stainings were performed to evaluate collagen, glycosamynoglycans and elastic fibers. Immunohistochemistry analyses were carried out to assess collagen I, collagen III and hyaluronan. The percentage area of collagen was significantly higher with aging (p < 0.01 in humans, p < 0.001 in mice), mainly due to an increase in collagen I, with no differences in collagen III (p > 0.05). The percentage area of elastic fibers in the perimysium was significantly lower (p < 0.01) in elderly men, together with a significant decrease in hyaluronan content both in humans and in mice. No significant differences were detected according to gender. The accumulation of collagen I and the lower levels of hyaluronan and elastic fibers with aging could cause a stiffening of the muscles and a reduction of their adaptability.

show abstract

Section: Discussionsupporting

confidence: 79%

The Effects of Aging on the Intramuscular Connective Tissue

Fede

Fan

Pirri

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…In these studies, MSI has been, for instance, used to study eyesight degeneration, 145,146 for visualizing oxidative stress in the brain, 147 or to investigate skin aging. 148,149 Other expanding areas of MSIbeyond biomedicine and which address urgent global challenges of the next decade (climate change and agriculture/food production)are its application in plant biology and food analysis.…”

Section: Discussionmentioning

confidence: 99%

“…In these studies, MSI has been, for instance, used to study eyesight degeneration, 145,146 for visualizing oxidative stress in the brain, 147 or to investigate skin aging. 148,149…”

Section: Discussionmentioning

confidence: 99%

State-of-the-art mass spectrometry imaging applications in biomedical research

Krestensen,

Heeren,

Balluff

2023

Analyst

View full text Add to dashboard Cite

show abstract

“…Quantinetix 1.7.1 (ImaBiotech, France) software was used to plot the map distribution of HA. The massto-charge ratio (m/z) of 1180.29 corresponds to HA after a standardized enzymatic digestion [43]. Histological coloration allowed localizing the epidermis and the dermis on skin sections and by comparison, with the MALDI molecular image, to calculate the relative HA amounts according to the depth of the sample.…”

Section: Cosmetic Molecules Of Interest and Their Diffusion Across A ...mentioning

confidence: 99%

Boost of cosmetic active ingredient penetration triggered and controlled by the delivery of kHz plasma jet on human skin explants

et al. 2023

Self Cite

View full text Add to dashboard Cite

This work reports on the demonstration of the penetration of cosmetic active ingredients (caffeine and hyaluronic acid) in human skin explants following safe and controlled plasma jet exposure. First, temperature increase and immunohistochemistry in the stratum corneum and epidermis were characterized to check the safe delivery of plasma jets and to select two operation regimes at 1 and 20 kHz. Plasma exposure for tens of seconds is shown to induce transient modulations of skin pH, transepidermal water loss, and skin wettability, revealing a reversible skin barrier function modulation. Then, it is demonstrated that plasma exposure significantly accelerates the penetration of active ingredients. The tuning of the plasma jet pulse repetition rate allows controlling the penetration kinetics. Such ex vivo results agree with previous in vitro experiments also exhibiting a transient permeabilization time window. A preliminary demonstration of human skin wettability modulation with a low-power, user-friendly dielectric barrier discharge setup is documented, opening perspectives for plasma-based home cosmetic care device development. To the best of our knowledge, this work is one of the first demonstrations of safe and controlled plasma-assisted active ingredients’ skin penetration in the context of cosmetic applications.

show abstract

Hyaluronic acid detection and relative quantification by mass spectrometry imaging in human skin tissues

Cited by 17 publications

References 28 publications

The Effects of Aging on the Intramuscular Connective Tissue

The Effects of Aging on the Intramuscular Connective Tissue

State-of-the-art mass spectrometry imaging applications in biomedical research

Boost of cosmetic active ingredient penetration triggered and controlled by the delivery of kHz plasma jet on human skin explants

Contact Info

Product

Resources

About