Hyaluronic acid of high molecular weight inhibits proliferation and induces cell death in U937 macrophage cells

Inhibition of Hyaluronan Synthase-3 Decreases Subcutaneous Colon Cancer Growth by Increasing Apoptosis

“…In contrast, high molecular weight HA appears to decrease apoptosis in a human epithelial cell line [20]. Sheehan, et al , have shown that high molecular weight HA induces apoptosis and decreases proliferation in macrophages, a result similar to our observation in the HCT116 cells [47]. Because HAS isozymes synthesize and retain different length polymers [48], it is possible that our selective inhibition of HAS3 resulted in a decrease in a specific HA polymer that inhibits apoptosis.…”

Hyaluronan Inhibits Prostaglandin E2 Production via CD44 in U937 Human Macrophages

“…U937 cells express CD44 (Sheehan et al 2004) and ICAM-1 (Yang et al 2002), both of which can bind HA. While our previous study has demonstrated that HA suppresses NF-κ B activation via ICAM-1 in LPS-stimulated U937 cells (Yasuda 2007), the present With or without preincubation with anti-CD44 or non-specific IgG for 1 hour at 10 µ g/ml, adherent U937 macrophages were incubated for 1 hour with or without 2,700-kDa HA at 1 mg/ml.…”

“…U937 cells have been shown to express CD44 capable of binding HA (Sheehan et al 2004). Fluorescence cytochemistry was performed to confirm that HA can bind Fig.…”

Amphoteric hyaluronic acid derivative for targeting gene delivery

“…Moreover, the excellent mucoadhesive properties of higher molecular weight HA were helpful to the cell uptake of the carrier. However, high molecular weight HA greatly inhibits macrophage population growth by the dual actions of impeding cell proliferation and inducing apoptosis at high concentration [37]. It is important to select the proper molecular weight HA for effective targeting gene delivery.…”