Hyaluronic acid/poly(ethylenimine) polyelectrolyte multilayer coatings for siRNA-mediated local gene silencing

Koenig, Olivia; Neumann, Beate; Schlensak, Christian; Wendel, Hans Peter; Nolte, Andrea

doi:10.1371/journal.pone.0212584

Cited by 13 publications

(14 citation statements)

References 57 publications

(45 reference statements)

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“…Ternary nanocomplexes of HA conjugates with poly(hexamethylene biguanide) and chitosan were to deliver an anti-KRAS siRNA to colorectal cancer cells, exploiting the interaction of HA with CD44 as a means to achieve selective targeting of CD44-positive cancer cells [188]. Multilayers of HA/PEI were also constructed to deliver siRNA and gene silencing [189].…”

Section: Natural Carbohydrate Polymers For Gene Deliverymentioning

confidence: 99%

Biodegradable Polymers for Gene Delivery

2019

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The cellular transport process of DNA is hampered by cell membrane barriers, and hence, a delivery vehicle is essential for realizing the potential benefits of gene therapy to combat a variety of genetic diseases. Virus-based vehicles are effective, although immunogenicity, toxicity and cancer formation are among the major limitations of this approach. Cationic polymers, such as polyethyleneimine are capable of condensing DNA to nanoparticles and facilitate gene delivery. Lack of biodegradation of polymeric gene delivery vehicles poses significant toxicity because of the accumulation of polymers in the tissue. Many attempts have been made to develop biodegradable polymers for gene delivery by modifying existing polymers and/or using natural biodegradable polymers. This review summarizes mechanistic aspects of gene delivery and the development of biodegradable polymers for gene delivery.

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Section: Natural Carbohydrate Polymers For Gene Deliverymentioning

confidence: 99%

Biodegradable Polymers for Gene Delivery

2019

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“…This anionic biopolymer has great cytocompatibility and biodegradability. Because of these properties, it has been used in PEM coatings on biomaterials (Koenig, Neumann, Schlensak, Wendel, & Nolte, ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers

Sabino

Kauk

Madruga

et al. 2020

J Biomedical Materials Res

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Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices. For decades, titanium-based implants have been largely used for different medical applications. However, titanium can neither suppress blood coagulation, nor prevent bacterial infections. To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed. The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements. To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.The antibacterial activity was investigated against Gram-negative P. aeruginosa and Gram-positive S. aureus. Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM. The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation. Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation. The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.

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“…Positively and negatively charged polyelectrolytes can be alternately adsorbed via electrostatic adsorption to fabricate a polyelectrolyte multilayer (PEM) film; this is referred to as layer-by-layer (LbL) technology [29,30]. PEMs with controlled loading and release profiles has been experimentally used to deliver growth factors [31,32], DNA molecules [33,34], RNA molecules [35,36], etc.…”

Section: Introductionmentioning

confidence: 99%

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Liu

et al. 2020

J Nanobiotechnol

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Background The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. Methods Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. Results The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. Conclusions The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

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Hyaluronic acid/poly(ethylenimine) polyelectrolyte multilayer coatings for siRNA-mediated local gene silencing

Cited by 13 publications

References 57 publications

Biodegradable Polymers for Gene Delivery

Biodegradable Polymers for Gene Delivery

Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

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