Hyaluronic acid production and molecular weight improvement by redirection of carbon flux towards its biosynthesis pathway

Shah, Mihir V.; Badle, Sneh Sanjay; Ramachandran, K.B.

doi:10.1016/j.bej.2013.09.013

Cited by 43 publications

(34 citation statements)

References 29 publications

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“…However, in our results the relationship between biomass and HA production was positive. This effect was also observed by Shah and co-workers [16]. Pires and Santana [15] explained that the high HA concentration in an alkaline medium might be due to the exposition of the microorganisms to the stress condition in which the cells produce the capsule as a way to shield themselves.…”

Section: Effect Of Ph On the Bioreactorsupporting

confidence: 67%

“…The highest polymer productions were observed in media containing casein, 3.32 g.L −1 [45] and 5.0 g.L −1 [46], and media supplemented with amino acid, oxygen vector, and other additives that aimed to redirect the carbon flux to HA production. Adding 5 g.L −1 glutamine and 25 μM sodium iodoacetate increased the HA concentration to 5.0 g.L −1 from 2.0 g.L −1 in a control run [16]. Lai et al [47] studied the potential of oxygen vectors for enhancing HA biosynthesis and obtained a high production of 4.25 g.L −1 when 0.5 % (v/v) n-hexadecane was added.…”

Section: Effect Of Ph On the Bioreactormentioning

confidence: 99%

“…Thus, one important goal of the fermentation process is to study a new cost-effective culture medium that can increase HA production. In most studies, glucose is used as the primary carbon source for HA production [15,16]. The possibility of using byproducts rich in carbon and other essential nutrients for microorganism growth in industry is underexplored.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

Pan

Pereira

Silva

et al. 2016

Appl Biochem Biotechnol

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Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L total sugar) pretreated with activated charcoal and yeast extract (50 g.L). The HA produced exhibited a high molecular weight of 1.35 × 10 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid.

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Section: Effect Of Ph On the Bioreactorsupporting

confidence: 67%

Section: Effect Of Ph On the Bioreactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

Pan

Pereira

Silva

et al. 2016

Appl Biochem Biotechnol

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“…The third method is to add the sodium iodoacetate, tryptophan and pyruvate as glycolytic inhibitors into the medium to reduce lactic acid production, which can increase the molecular weight of HA effectively (Shah et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…The second method includes using glucose-limited medium; the metabolism may shift from lactic acid to a mixture of formate, acetate and ethanol, thereby realizing a yield of three ATP per mole glucose (Chong et al 2005). The third method is to add the sodium iodoacetate, tryptophan and pyruvate as glycolytic inhibitors into the medium to reduce lactic acid production, which can increase the molecular weight of HA effectively (Shah et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Highly efficient production of hyaluronic acid byStreptococcus zooepidemicusR42 derived from heterologous expression of bacterial haemoglobin and mutant selection

Lü

Zhu

Sun

et al. 2016

Lett Appl Microbiol

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Hyaluronic acid (HA) has been used for a wide range of applications in health, cosmetic and clinical fields. During fermentation of Streptococcus to produce HA, 80-85% of the carbon source is used to produce lactic acid and acetic acid, and only approx. 5 and 10% of the carbon source is used to produce HA and biomass respectively. Here, we expressed bacteria haemoglobin (Vhb) in Streptococcus zooepidemicus, which can dramatically inhibit lactic acid production. After NTG treatments and selection programmes, we identified a mutant strain with highly efficient hyaluronic acid production (6·7 g l(-1) ) under economic fermentation conditions.

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Chromosomal integration of hyaluronic acid synthesis (has) genes enhances the molecular weight of hyaluronan produced in Lactococcus lactis

Hmar

Prasad

Jayaraman

et al. 2014

Biotechnology Journal

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Microbial production of hyaluronic acid (HA) is an attractive substitute for extraction of this biopolymer from animal tissues. Natural producers such as Streptococcus zooepidemicus are potential pathogens; therefore, production of HA by recombinant bacteria that are generally recognized as safe (GRAS) organisms is a viable alternative that is being extensively explored. However, plasmid-based expression systems for HA production by recombinant bacteria have the inherent disadvantage of reduced productivity because of plasmid instability. To overcome this problem, the HA synthesis genes (hasA-hasB and hasA-hasB-hasC) from has-operon of S. zooepidemicus were integrated into the chromosome of Lactococcus lactis by site-directed, double-homologous recombination developing strains VRJ2AB and VRJ3ABC. The chromosomal integration stabilized the genes and obviated the instability observed in plasmid-expressed recombinant strains. The genome-integrated strains produced higher molecular weight (3.5-4 million Dalton [MDa]) HA compared to the plasmid-expressed strains (2 MDa). High molecular weight HA was produced when the intracellular concentration of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) and uridine diphosphate-glucuronic acid (UDP-GlcUA) was almost equal and hasA to hasB ratio was low. This work suggests an optimal approach to obtain high molecular weight HA in recombinant strains.

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Hyaluronic acid production and molecular weight improvement by redirection of carbon flux towards its biosynthesis pathway

Cited by 43 publications

References 29 publications

Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses

Highly efficient production of hyaluronic acid byStreptococcus zooepidemicusR42 derived from heterologous expression of bacterial haemoglobin and mutant selection

Chromosomal integration of hyaluronic acid synthesis (has) genes enhances the molecular weight of hyaluronan produced in Lactococcus lactis

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