Hyaluronic acid production and purification techniques: a review

Rossatto, Andressa; Santos, Jandler Trocado dos; Arlindo, Maurício Zimmer Ferreira; Morais, Matheus Saraiva de; Souza, Taiana Denardi de; Ogrodowski, Christiane Saraiva

doi:10.1080/10826068.2022.2042822

Cited by 10 publications

(2 citation statements)

References 46 publications

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“…There is evidence in the scientific literature that polymers such as P407, NaCMC and HPMC can be autoclaved [ 68 , 69 , 70 ]. Autoclaving hyaluronic acid at high temperatures often results in lower molecular weight derivatives, which may affect the viscosity of the formulation [ 71 ]. HA is an unstable molecule at temperatures above 100 °C due to damage to the interchain bonds [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Balsam Poplar Buds: Extraction of Potential Phenolic Compounds with Polyethylene Glycol Aqueous Solution, Thermal Sterilization of Extracts and Challenges to Their Application in Topical Ocular Formulations

et al. 2022

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Phenolic compounds of natural origin have been valued for their beneficial effects on health since ancient times. During our study, we performed the extraction of phenolic compounds from balsam poplar buds using different concentrations of aqueous polyethylene glycol 400 solvents (10–30% PEG400). The aqueous 30% PEG400 extract showed the best phenolic yield. The stability of the extract during autoclave sterilization was evaluated. The extract remained stable under heat sterilization. Ophthalmic formulations are formed using different concentrations (8–15%) of poloxamer 407 (P407) together with hydroxypropyl methylcellulose (0.3%), sodium carboxymethyl cellulose (0.3%) or hyaluronic acid (0.1%). Physicochemical parameters of the formulations remained significantly unchanged after sterilization. Formulations based on 12% P407 exhibited properties characteristic of in situ gels, the gelation point of the formulations was close to the temperature of the cornea. After evaluating the amount of released compounds, it was found that, as the concentration of polymers increases, the amount of released compounds decreases. Formulations based on 15% P407 released the least biologically active compounds. Sterilized formulations remained stable for 30 days.

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Section: Discussionmentioning

confidence: 99%

Balsam Poplar Buds: Extraction of Potential Phenolic Compounds with Polyethylene Glycol Aqueous Solution, Thermal Sterilization of Extracts and Challenges to Their Application in Topical Ocular Formulations

et al. 2022

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“…The purification of GAGs free from contaminant biomolecules is routine and undemanding for both ex vivo and in vitro biological sources, with well-established methodologies available within the scientific literature ,− for both large- and small-scale purifications. Subsequent sample preparation for ATR-FTIR is facile and quick, and can employ solid material, avoiding dissolution and obviating any need for skilled NMR technical assistance, making the current approach both economical and accessible.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Heparin Samples by Attenuated Total Reflectance Fourier-Transform Infrared Spectroscopy in the Solid State

et al. 2023

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Heparin is a polydisperse, heterogeneous polysaccharide of the glycosaminoglycan (GAG) class that has found widespread clinical use as a potent anticoagulant and is classified as an essential medicine by the World Health Organization. The importance of rigorous monitoring and quality control of pharmaceutical heparin was highlighted in 2008, when the existing regulatory procedures failed to identify a life-threatening adulteration of pharmaceutical heparin with oversulfated chondroitin sulfate (OSCS). The subsequent contamination crisis resulted in the exploration of alternative approaches for which the use of multidimensional nuclear magnetic resonance (NMR) spectroscopy techniques and multivariate analysis emerged as the gold standard. This procedure is, however, technically demanding and requires access to expensive equipment. An alternative approach, utilizing attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) combined with multivariate analysis, has been developed. The method described enables the differentiation of diverse GAG samples, the classification of samples of distinct species provenance, and the detection of both established heparin contaminants and alien polysaccharides. This methodology has sensitivity comparable to that of NMR and can facilitate the rapid, cost-effective monitoring and analysis of pharmaceutical heparin. It is therefore suitable for future deployment throughout the supply chain.

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