Hyaluronidases, a group of glycosidases: Current and future perspectives

El-Safory, Nermeen S.; Fazary, Ahmed E.; Lee, Cheng‐Kang

doi:10.1016/j.carbpol.2010.02.047

Cited by 100 publications

(43 citation statements)

References 205 publications

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“…1). 1,2 Mammalian HAase (E.C. 3.2.1.35) hydrolyzes b-1,4 glycosidic linkages, producing HA fragments with N-acetyl-D-glucosamine at the reducing end, and also shows a limited activity to degrade chondroitins, chondroitin sulfates, and dermatan sulfates.…”

mentioning

confidence: 99%

Interpreting the behavior of concentration–response curves of hyaluronidase inhibitors under DMSO-perturbed assay conditions

Tomohara

Ito

Onikata

et al. 2016

Bioorganic & Medicinal Chemistry Letters

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mentioning

confidence: 99%

Interpreting the behavior of concentration–response curves of hyaluronidase inhibitors under DMSO-perturbed assay conditions

Tomohara

Ito

Onikata

et al. 2016

Bioorganic & Medicinal Chemistry Letters

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“…Among the proteins that are able to modify the cell permeability, are the hyaluronidases. Hyaluronidases are glycosidases (El-Safory et al, 2010) capable of hydrolysing the hyaluronic acid and, thus, digest partially the extracellular matrix (Hoffman, 2006), increasing the infiltration and, possibly, the action of other compounds of the venom on cellular structures. The hyaluronic acid is a polysaccharide of high molecular weight found in the extracellular matrix, especially in connective tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Viability% ( and moisturizing agent of connective tissues (El-Safory et al, 2010). According to Wahby et al (2012), hyaluronidase increases the permeability of the cell membranes and causes a reduction in the viscosity of the fluids injected into the tissues.…”

Section: Treatmentsmentioning

confidence: 99%

Cytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic effects of the venom of the wasp Polybia paulista

Hoshina

Santos

Palma

et al. 2013

Toxicon

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Hymenoptera venoms are constituted by a complex mixture of chemically or pharmacologically bioactive agents, such as phospholipases, hyaluronidases and mastoparans. Venoms can also contain substances that are able to inhibit and/or diminish the genotoxic or mutagenic action of other compounds that are capable of promoting damages in the genetic material. Thus, the present study aimed to assess the effect of the venom of Polybia paulista, a neotropical wasp, by assays with HepG2 cells maintained in culture. The cytotoxic potential of the wasp venom, assessed by the methyl thiazolyl tetrazolium assay (MTT assay), was tested for the concentrations of 10 μg/mL, 5 μg/mL and 1 μg/mL. As these concentrations were not cytotoxic, they were used to evaluate the genotoxic (comet assay) and mutagenic potential (micronucleus test) of the venom. In this study, it was verified that these concentrations induced damages in the DNA of the exposed cells, and it was necessary to test lower concentrations until it was found those that were not considered genotoxic and mutagenic. The concentrations of 1 ng/mL, 100 pg/mL and 10 pg/mL, which did not induce genotoxicity and mutagenicity, were used in four different treatments (post-treatment, pre-treatment, simultaneous treatment with and without incubation), in order to evaluate if these concentrations were able to inhibit or decrease the genotoxic and mutagenic action of methyl methanesulfonate (MMS). None of the concentrations was able to inhibit and/or decrease the MMS activity. The genotoxic and mutagenic activity of the venom of P. paulista could be caused by the action of phospholipase, mastoparan and hyaluronidase, which are able to disrupt the cell membrane and thereby interact with the genetic material of the cells or even facilitate the entrance of other compounds of the venom that can act on the DNA. Another possible explanation for the genotoxicity and mutagenicity of the venom can be the presence of substances able to trigger inflammatory process and, consequently, generate oxygen reactive species that can interact with the DNA of the exposed cells.

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“…It can also be produced from the bacteriophage of S. pyogenes with wide substrate specificity . The degradation of polymeric HA and other glycosaminoglycans by HA lyases plays an essential roles in many biological processes (El-Safory, Fazary, & Lee, 2010;Kostyukova, Volkova, Ivanova, & Kvetnaya, 1995;Linhardt, Galliher, & Cooney, 1986;Lui & Nizet, 2004;Polissi et al, 1998;Starrl & Engleberg, 2006).…”

Section: Introductionmentioning

confidence: 95%

“…It was known that, the HA lyase isolated from group A Streptococci or group B Streptococci shows a significant activities towards chondroitin and/or chondroitin 4/6-sulfate, besides cleaving HA (El-Safory et al, 2010). Among the HA lyases known, the most considered ones are those secreted by strains of group B Streptococci with a broad substrate specificity such as HA and certain chondroitin sulfates (CS) (Lui & Nizet, 2004).…”

Section: Introductionmentioning

confidence: 99%

Characterization of hyaluronate lyase from Streptococcus pyogenes bacteriophage H4489A

El-Safory

Lee

2011

Carbohydrate Polymers

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Hyaluronidases, a group of glycosidases: Current and future perspectives

Cited by 100 publications

References 205 publications

Interpreting the behavior of concentration–response curves of hyaluronidase inhibitors under DMSO-perturbed assay conditions

Interpreting the behavior of concentration–response curves of hyaluronidase inhibitors under DMSO-perturbed assay conditions

Cytotoxic, genotoxic/antigenotoxic and mutagenic/antimutagenic effects of the venom of the wasp Polybia paulista

Characterization of hyaluronate lyase from Streptococcus pyogenes bacteriophage H4489A

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