Hyaluronidases and their inhibitors in the serum of colorectal carcinoma patients

Kolliopoulos, Constantinos; Bounias, Dimitrios; Bouga, Helen; Kyriakopoulou, Dora; Stavropoulos, M.; Vynios, Demitrios H.

doi:10.1016/j.jpba.2013.05.037

Cited by 17 publications

(11 citation statements)

References 26 publications

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“…The physiological hyaluronidase concentration depends on the condition and location in the body [24,25]. In addition, there are hyaluronidase inhibitors in serum [26]. An important finding in this study is the possibility of tailoring the degradation rate by changing HRP, GOx, and HA-Ph concentrations.…”

Section: In Vitro Examinationsmentioning

confidence: 85%

Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

2015

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Postsurgical peritoneal adhesion is a serious surgical complication. In this paper, we report a novel system for preventing it through an on-tissue surface formation of a biodegradable and biocompatible hyaluronic acid-based hydrogel by administration of a pre-hydrogel aqueous solution. The in situ hydrogelation is mediated by a cascade enzyme reaction of glucose oxidase (GOx) and horseradish peroxidase (HRP) initiated by contacting with body fluid containing glucose. The efficiency of the system was confirmed by applying the system to animals with bowel abrasion-abdominal sidewall defects.

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Section: In Vitro Examinationsmentioning

confidence: 85%

Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

2015

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“…Collagen type I (Col-I) is a major ECM protein ( 14 ), thus, it was selected to be investigated in order to elucidate the role of the ECM in cancer development. The current study also examined the expression levels of hyaluronidase-1 (Hyal-1), a major tumor-derived hyaluronidase ( 15 ). Dysregulation of different ECM components may favor the neoplastic process ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

Huang

Gong

et al. 2016

Molecular Medicine Reports

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The present study aimed to observe the varying expression of biomarkers in the microenvironment adjacent to colorectal cancer lesions to provide additional insight into the functions of microenvironment components in carcinogenesis and present a novel or improved indicator for early diagnosis of cancer. A total of 144 human samples from three different locations in 48 patients were collected, these locations were 10, 5 and 2 cm from the colorectal cancer lesion, respectively. The biomarkers analyzed included E-cadherin, cytokeratin 18 (CK18), hyaluronidase-1 (Hyal-1), collagen type I (Col-I), Crumbs3 (CRB3), vimentin, proteinase activated receptor 3 (PAR-3), α-smooth muscle actin (α-SMA), cyclin D1 (CD1) and cluster of differentiation (CD)133. In addition, crypt architecture was observed. Related functional analysis of proteins was performed using hierarchical index cluster analysis. More severe destroyed crypt architecture closer to the cancer lesions was observed compared with the 10 cm sites, with certain crypts degraded entirely. Expression levels of E-cadherin, CK18, CRB3 and PAR-3 were lower in 2 cm sites compared with the 10 cm sites (all P<0.001), while the expression levels of the other biomarkers in the 2 cm sites were increased compared with 10 cm sites (all P<0.0001). Notably, the expression of CK18 in 2 cm sites was higher than in the 5 cm site (P<0.0001), which was different from the expression of E-cadherin, CRB3 and PAR-3. The expression levels of Hyal-1 and Col-I at the 2 cm sites were lower than that of the 5 cm sites (P>0.05 and P=0.0001, respectively), while the expression of vimentin, α-SMA, CD1 and CD133 were not. Hyal-1 and Col-I may be independently important in cancer initiation in the tumor microenvironment. The results of the present study suggest that the biomarkers in the tissue microenvironment are associated with early tumorigenesis and may contribute to the development of carcinomas. These observations may be useful for early diagnosis of colorectal cancer.

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“…Hyal1 was detected in serum samples from colorectal carcinoma patients, where serum Hyal1 was reduced in cancer patients compared to levels in normal samples (Kolliopoulos et al, 2013). This phenomenon could be attributable to localization of the free Hyal1 at the primary tumor site rather than in the circulation.…”

Section: : Hyaluronidase Expression In Cancermentioning

confidence: 99%

Emerging Roles for Hyaluronidase in Cancer Metastasis and Therapy

McAtee

Barycki

Simpson³

2014

Advances in Cancer Research

169

140

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Hyaluronidases are a family of five human enzymes that have been differentially implicated in the progression of many solid tumor types, both clinically and in functional studies. Advances in the past five years have clarified many apparent contradictions, (1) by demonstrating that specific hyaluronidases have alternative substrates to hyaluronan (HA) or do not exhibit any enzymatic activity, (2) that high molecular weight HA polymers elicit signaling effects that are opposite those of the hyaluronidase-digested HA oligomers, and (3) that it is actually the combined overexpression of HA synthesizing enzymes with hyaluronidases that confers tumorigenic potential. This review examines the literature supporting these conclusions and discusses novel mechanisms by which hyaluronidases impact invasive tumor cell processes. In addition, a detailed structural and functional comparison of the hyaluronidases is presented with insights into substrate selectivity and potential for therapeutic targeting. Finally, technological advances in targeting hyaluronidase for tumor imaging and cancer therapy are summarized.

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Hyaluronidases and their inhibitors in the serum of colorectal carcinoma patients

Cited by 17 publications

References 26 publications

Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

Emerging Roles for Hyaluronidase in Cancer Metastasis and Therapy

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