Hybrid Adipogenic Implants from Adipose Stem Cells for Soft Tissue Reconstruction <i>In Vivo</i>

Moioli, Eduardo K.; Chen, Mo; Yang, Rujing; Shah, Bhavya; Wu, June K.; Mao, Jeremy J.

doi:10.1089/ten.tea.2010.0157

Cited by 20 publications

(16 citation statements)

References 29 publications

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“…ASCs were isolated from subcutaneous liposuction aspirates and digested with 0.25 mg collagenase (200 U/mg)/ml of Krebs‐Ringer‐bicarbonate solution (Sigma) at 37°C for 40 min. Following centrifugation, the stromal‐vascular fraction was isolated and plated, per our prior methods (34).…”

Section: Methodsmentioning

confidence: 99%

Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cellsin vitro

et al. 2011

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Cell transplantation has been well explored for cartilage regeneration. We recently showed that the entire articular surface of a synovial joint can regenerate by endogenous cell homing and without cell transplantation. However, the sources of endogenous cells that regenerate articular cartilage remain elusive. Here, we studied whether cytokines not only chemotactically recruit adipose stem cells (ASCs), mesenchymal stem cells (MSCs), and synovium stem cells (SSCs) but also induce chondrogenesis of the recruited cells. Recombinant human transforming growth factor-β3 (TGF-β3; 100 ng) and/or recombinant human stromal derived factor-1β (SDF-1β; 100 ng) was control released into an acellular collagen sponge cube with underlying ASCs, MSCs, or SSCs in monolayer culture. Although all cell types randomly migrated into the acellular collagen sponge cube, TGF-β3 and/or SDF-1β recruited significantly more cells than the cytokine-free control group. In 6 wk, TGF-β3 alone recruited substantial numbers of ASCs (558±65) and MSCs (302±52), whereas codelivery of TGF-β3 and SDF-1β was particularly chemotactic to SSCs (400±120). Proliferation of the recruited cells accounted for some, but far from all, of the observed cellularity. TGF-β3 and SDF-1β codelivery induced significantly higher aggrecan gene expression than the cytokine-free group for ASCs, MSCs, and SSCs. Type II collagen gene expression was also significantly higher for ASCs and SSCs by SDF-1 and TGF-β3 codelivery. Remarkably, the expression of aggrecan and type II collagen was detected among all cell types. Thus, homing of multiple stem/progenitor cell populations may potentially serve as an alternative or adjunctive approach to cell transplantation for cartilage regeneration.

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Section: Methodsmentioning

confidence: 99%

Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cellsin vitro

et al. 2011

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“…In particular, ASCs are increasingly considered for reconstructive procedures following surgery for breast cancer, which compose the majority of the 93,000 breast reconstructions performed in the United States per year (3). ASCs offer several advantages over commonly utilized silicone and saline implants including their ability to regenerate functional adipose tissue, which reconstitutes a large fraction of the breast (4,5); however, malignantly transformed cells may be present in breast cancer survivors without manifest disease, and it remains unclear whether implanted ASCs may increase the risk of tumor development and relapse (6) by establishing a microenvironment conducive to nascent or recurrent tumorigenesis.…”

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confidence: 99%

Implanted adipose progenitor cells as physicochemical regulators of breast cancer

Chandler

Seo

Califano

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

136

118

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Multipotent adipose-derived stem cells (ASCs) are increasingly used for regenerative purposes such as soft tissue reconstruction following mastectomy; however, the ability of tumors to commandeer ASC functions to advance tumor progression is not well understood. Through the integration of physical sciences and oncology approaches we investigated the capability of tumor-derived chemical and mechanical cues to enhance ASC-mediated contributions to tumor stroma formation. Our results indicate that soluble factors from breast cancer cells inhibit adipogenic differentiation while increasing proliferation, proangiogenic factor secretion, and myofibroblastic differentiation of ASCs. This altered ASC phenotype led to varied extracellular matrix (ECM) deposition and contraction thereby enhancing tissue stiffness, a characteristic feature of breast tumors. Increased stiffness, in turn, facilitated changes in ASC behavior similar to those observed with tumor-derived chemical cues. Orthotopic mouse studies further confirmed the pathological relevance of ASCs in tumor progression and stiffness in vivo. In summary, altered ASC behavior can promote tumorigenesis and, thus, their implementation for regenerative therapy should be carefully considered in patients previously treated for cancer.mechanobiology | desmoplasia | angiogenesis | tissue engineering

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“…This regenerative potential of adipose tissue (AT) and cultured ASCs was quickly tested on the healing of chronic wounds. When injected around the wound they accelerated complete wound closure [32][33][34][35]. Furthermore, it was shown that ASCs cultured in a monolayer secrete growth factors that have an effect on fibroblast and keratinocyte proliferation [36][37][38][39][40][41][42][43][44] and an immunomodulatory effect on the inflammatory response (reviewed by [45,46]).…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived Stem Cells Promote Skin Homeostasis and Prevent its Senescence in an In vitro Skin Model

Metral

Santos²,

Thépot³

et al. 2014

J Stem Cell Res Ther

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Objectives: Skin aging is subject of many studies in esthetic surgery including several morphological changes like decrease of epidermis thickness and of cell proliferative potential. ASC, mesenchymal stem cells derived from adipose tissue, have been used in regenerative and reparative surgery as well as anti-aging solution. The aim of this study was to highlight the influence of ASCs on skin aging and healing in an in vitro skin model. Methods: Using an skin equivalent (SE) model prepared without or with ASCs in different proportion (25% or 50% of ASCs), beneficial influence of ASCs on epidermal regeneration via markers of proliferative and differentiative potential and on quality of the dermis via markers of dermal protein synthesis was analyzed. In addition, an extendedtime cultured model mimicking skin ageing was used to demonstrate ASCs influence on skin aging via markers of the quality of the epidermis and dermis as well as via a marker of senescence. Results: After 42 days of culture, SEs prepared with 25% of ASCs were thicker, presented a better proliferative potential showed by a higher number of Ki67 positive cells, and a better differentiation in epidermis. A better fibroblast synthesis in dermis was also showed. Skin ageing was studied by prolonging culture time : SEs prepared with 25% of ASCs showed remained Ki67 positive cells and a low level of senescent marker, p16, labeling, whereas SEs with fibroblasts alone were very thin, free of proliferative cells and with a high p16 expression. Conclusions: To conclude, adding ASCs in low proportion (25%) improved quality of the epidermis and dermis preventing senescence of the SE model. This confirms clinical results and supports their anti-aging effect and their potential in wound healing. In addition, this model provides a tool to elucidate the mode of action of ASCs on healing and skin aging.

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Hybrid Adipogenic Implants from Adipose Stem Cells for Soft Tissue Reconstruction In Vivo

Cited by 20 publications

References 29 publications

Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cellsin vitro

Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cellsin vitro

Implanted adipose progenitor cells as physicochemical regulators of breast cancer

Adipose-derived Stem Cells Promote Skin Homeostasis and Prevent its Senescence in an In vitro Skin Model

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