Hybrid bilayer membranes on metallurgical polished aluminum

Sabirovas, Tomas; Valiūnienė, Aušra; Valinčius, Gintaras

doi:10.1038/s41598-021-89150-2

Cited by 8 publications

(3 citation statements)

References 82 publications

(55 reference statements)

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“…The current understanding of the pore formation dynamics of MEL is that these αhelical monomers, at certain concentrations, spontaneously insert into the lipid bilayer and aggregate to form a transmembrane cylindrical pore. It has been reported that MEL requires a threshold concentration of peptide-lipid ratio to form pores [37,38]. Studies show that this membrane-damaging activity of MEL is high in model membranes containing lower levels of cholesterol [39][40][41].…”

Section: Mel Reconstituted Tblmsmentioning

confidence: 99%

Effect of pH on Electrochemical Impedance Response of Tethered Bilayer Lipid Membranes: Implications for Quantitative Biosensing

Shivabalan,

Ambrulevicius,

Talaikis

et al. 2023

Chemosensors

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Tethered bilayer lipid membranes (tBLMs) are increasingly used in biosensor applications where electrochemical impedance spectroscopy (EIS) is the method of choice for amplifying and recording the activity of membrane-damaging agents such as pore-forming toxins or disrupting peptides. While the activity of these biological agents may depend on the pH of the analytes, there is increasing evidence that the sensitivity of tethered bilayer sensors depends on the pH of the solutions. In our study, we addressed the question of what are the fundamental reasons for the variability of the EIS signal of the tBLMs with pH. We designed an experiment to compare the EIS response of tBLMs with natural membrane defects and two different membrane disruptors: vaginolysin and melittin. Our experimental design ensured that the same amount of protein and peptide was present in the tBLMs, while the pH was varied by replacing the buffers with different pH values. Using a recently developed EIS data analysis algorithm from our research group, we were able to demonstrate that, in contrast to previous literature which relates the variability of tBLM, EIS response to the variation in defect density, the main reason for the observed variability in EIS response is the change in the sub-membrane properties of tBLMs with pH. Using surface-enhanced infrared absorption spectroscopy (SEIRAS), we have shown that pH changes from neutral to slightly acidic leads to an expulsion of water, presumably bound to ions, from the sub-membrane reservoir, resulting in a marked decrease in the carrier concentration and specific conductance of the sub-membrane reservoir. Such a decrease is recorded by the EIS as a decrease in the conductance of the tBLM complex and affects the sensitivity of a biosensor. Our data provide important evidence of pH-sensitive effects that should be considered in both the development and operation of biosensors.

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Section: Mel Reconstituted Tblmsmentioning

confidence: 99%

Effect of pH on Electrochemical Impedance Response of Tethered Bilayer Lipid Membranes: Implications for Quantitative Biosensing

Shivabalan,

Ambrulevicius,

Talaikis

et al. 2023

Chemosensors

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“…The latter case is sometimes referred to as a hybrid lipid bilayer (HLB) and is understood to improve the ruggedness of lipid bilayer attachment [ 12 , 13 , 14 , 15 ]. In this case, HLB fabrication typically involves two main steps: (1) a hydrophobic self-assembled monolayer (SAM) is first formed on the material surface (usually gold) by utilizing amphipathic molecules with one side bearing a functional group that can attach covalently to the surface and the other side consisting of a hydrocarbon chain (or so-called “tail”; in general, such molecules have one tail while two-tail molecules are also possible) [ 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Streamlined Fabrication of Hybrid Lipid Bilayer Membranes on Titanium Oxide Surfaces: A Comparison of One- and Two-Tail SAM Molecules

et al. 2022

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There is broad interest in fabricating cell-membrane-mimicking, hybrid lipid bilayer (HLB) coatings on titanium oxide surfaces for medical implant and drug delivery applications. However, existing fabrication strategies are complex, and there is an outstanding need to develop a streamlined method that can be performed quickly at room temperature. Towards this goal, herein, we characterized the room-temperature deposition kinetics and adlayer properties of one- and two-tail phosphonic acid-functionalized molecules on titanium oxide surfaces in various solvent systems and identified optimal conditions to prepare self-assembled monolayers (SAMs), upon which HLBs could be formed in select cases. Among the molecular candidates, we identified a two-tail molecule that formed a rigidly attached SAM to enable HLB fabrication via vesicle fusion for membrane-based biosensing applications. By contrast, vesicles adsorbed but did not rupture on SAMs composed of one-tail molecules. Our findings support that two-tail phosphonic acid SAMs offer superior capabilities for rapid HLB coating fabrication at room temperature, and these streamlined capabilities could be useful to prepare durable lipid bilayer coatings on titanium-based materials.

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“…To assemble both hybrid and tethered bilayers various solid supports are used: sputtered Au and Ti/TiO 2 [1][2][3], polished Ti [4], Si/SiO 2 [5], SnO 2 doped with either fluorine, cadmium or indium [6][7][8], aluminium [9,10]. Most of these surfaces (except for SiO 2 ) exhibit a good electric conductance, thus providing application of the electrochemical techniques for protein membrane interaction studies.…”

Section: Introductionmentioning

confidence: 99%

Formation of mixed hybrid bilayer lipid membrane on transparent thin film oxide electrodes

Gabriūnaitė¹,

Zolubas²,

Valiūnienė³

2022

chemija

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In this work, we attempted to design phospholipid bilayers tethered to semiconducting metal oxide films. The phospholipid layers assembled on compact monocomponent self-assembled monolayers (SAM) on oxide films were accomplished earlier; however, previous studies indicated a poor ability of these layers to functionally reconstitute transmembrane proteins. Commercially available fluorine doped tin oxide (FTO) sheet glass was chosen as a model solid support. FTO surface was functionalised utilising by the mixture of the long and short chain hydrophobic silanes. Specifically, octadecyltrichlorosilane (OTS) and vinyltrimethoxysilane (VTS) were used to assemble mixed silane-based SAMs on FTO. Exposure of mixed SAMs to a solution of multilamellar vesicles of 1,2-dioleoylsn- glycero-3-phosphocholine (DOPC) and 40% cholesterol triggered the changes of electrochemical parameters commensurate with the formation of additional dielectric layers of phospholipids.

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Hybrid bilayer membranes on metallurgical polished aluminum

Cited by 8 publications

References 82 publications

Effect of pH on Electrochemical Impedance Response of Tethered Bilayer Lipid Membranes: Implications for Quantitative Biosensing

Effect of pH on Electrochemical Impedance Response of Tethered Bilayer Lipid Membranes: Implications for Quantitative Biosensing

Streamlined Fabrication of Hybrid Lipid Bilayer Membranes on Titanium Oxide Surfaces: A Comparison of One- and Two-Tail SAM Molecules

Formation of mixed hybrid bilayer lipid membrane on transparent thin film oxide electrodes

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