Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Lickiss, Bettina; Goßmann, Matthias; Linder, Peter; Thomas, Ulrich; Dragicevic, Elena; Lemme, Marta; George, Michael; Fertig, Niels; Stölzle‐Feix, Sonja

doi:10.3791/64283

Cited by 3 publications

(1 citation statement)

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“…The methodology by Lickiss et al 2 presents a hybrid contractility and impedance/extracellular field potential (EFP) technology, adding significant pro-maturation features to an industry-standard 96-well platform by using a soft, flexible, silicon-based cell culture substrate. The approach proved successful by re-establishing the physiological positive inotropic response to isoproterenol in commercially available healthy hiPSC-CMs, which is knowingly absent in the standard (stiff substrate) culture without the need for a 3D system.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Collection of Current Methods for Contractility and Electrophysiology Recordings in Human Cardiac Cells

Blinova

Pierson

Narkar

2023

JoVE

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ARTICLES DISCUSSED: 1. Gerges, N. A. et al. Measurement of heart contractility in isolated adult human primary cardiomyocytes. Journal of Visualized Experiments. (186), e64394 (2022). 2. Lickiss, B. et al. Hybrid cell analysis system to assess structural and contractile changes of human iPSC-derived cardiomyocytes for preclinical cardiac risk evaluation. Journal of Visualized Experiments. (188), e64283 (2022). 3. Feaster, T. K., Casciola, M., Narkar, A., Blinova, K. Evaluation of cardiac contractility modulation therapy in 2D human stem cell-derived cardiomyocytes. Journal of Visualized Experiments. (190), e64848 (2022). 4. Schaefer, J., Danker, T., Gebhardt, K., Kraushaar, U. Laser-induced action potential-like measurements of cardiomyocytes on microelectrode arrays for increased predictivity of safety pharmacology. Journal of Visualized Experiments. (187), e64355 (2022). 5. Berry, B. J. et al. Preclinical drug testing in scalable 3D engineered muscle tissues. Journal of Visualized Experiments. e64399 (2022).

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Section: Discussionmentioning

confidence: 99%