Hybrid cell constructs consisting of bioprinted cell‐spheroids

Kim, Won Jin; Kim, GeunHyung

doi:10.1002/btm2.10397

Cited by 6 publications

(5 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interspheroid spacing is an important factor dictating spheroid crosstalk and fusion. , Unlike monodisperse cells, which are homogeneously dispersed within a material, the spacing of spheroids in biomaterial constructs is often heterogeneous . In this study, we did not measure or regulate spheroid spacing; however, others have shown that myoblast spheroids spaced approximately 300–400 μm apart achieved improved cell alignment and greater differentiation compared with smaller or larger distances .…”

Section: Discussionmentioning

confidence: 86%

“…We tested the influence of spheroid density in the bioink to alter interspheroid spacing, as spacing between spheroids can influence both cell migration and paracrine signaling. , We encapsulated C2C12 spheroids in oxidized, RGD-modified alginate and precross-linked with 50 mM CaCl 2 in a 7:3 ratio to create a viscous bioink with the rheological and shear thinning characteristics needed for bioprinting, as demonstrated by previous data . Cylindrical constructs were printed with increasing cell densities of 10, 20, and 50 × 10 6 cells/mL, respectively (Figure A–C).…”

Section: Resultsmentioning

confidence: 99%

“…17,45 Unlike monodisperse cells, which are homogeneously dispersed within a material, the spacing of spheroids in biomaterial constructs is often heterogeneous. 39 In this study, we did not measure or regulate spheroid spacing; however, others have shown that myoblast spheroids spaced approximately 300−400 μm apart achieved improved cell alignment and greater differentiation compared with smaller or larger distances. 17 Others reported limited spreading when spheroids were more than 250 μm apart, suggesting there may be differences in spheroid fusion based on cell type, spheroid diameter, secretory profiles, and matrix interactions.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue

Johnson,

Filler,

Sethi

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Spheroids exhibit enhanced cell−cell interactions that facilitate improved survival and mimic the physiological cellular environment in vivo. Cell spheroids have been successfully used as building blocks for engineered tissues, yet the viability of this approach with skeletal muscle spheroids is poorly understood, particularly when incorporated into three-dimensional (3D) constructs. Bioprinting is a promising strategy to recapitulate the hierarchical organization of native tissue that is fundamental to its function. However, the influence of bioprinting on skeletal muscle cell spheroids and their function are yet to be interrogated. Using C2C12 mouse myoblasts and primary bovine muscle stem cells (MuSCs), we characterized spheroid formation as a function of duration and cell seeding density. We then investigated the potential of skeletal muscle spheroids entrapped in alginate bioink as tissue building blocks for bioprinting myogenic tissue. Both C2C12 and primary bovine MuSCs formed spheroids of similar sizes and remained viable after bioprinting. Spheroids of both cell types fused into larger tissue clusters over time within alginate and exhibited tissue formation comparable to monodisperse cells. Compared to monodisperse cells in alginate gels, C2C12 spheroids exhibited greater MyHC expression after 2 weeks, while cells within bovine MuSC spheroids displayed increased cell spreading. Both monodisperse and MuSC spheroids exhibited increased expression of genes denoting mid-and late-stage myogenic differentiation. Together, these data suggest that skeletal muscle spheroids have the potential for generating myogenic tissue via 3D bioprinting and reveal areas of research that could enhance myogenesis and myogenic differentiation in future studies.

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue

Johnson,

Filler,

Sethi

et al. 2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…The inclusion of cell spheroids during the printing process can further enhance cell-to-cell interactions [ 154 ]. For instance, human-derived stem cells and endothelial cells mixed with mineral oil form cell spheroids due to oil–aqueous interactions.…”

Section: The Fabrication Flow Of Customized Am Bone Scaffoldsmentioning

confidence: 99%

Customized Additive Manufacturing in Bone Scaffolds—The Gateway to Precise Bone Defect Treatment

Zhou,

See,

Sreenivasamurthy

et al. 2023

Research

View full text Add to dashboard Cite

In the advancing landscape of technology and novel material development, additive manufacturing (AM) is steadily making strides within the biomedical sector. Moving away from traditional, one-size-fits-all implant solutions, the advent of AM technology allows for patient-specific scaffolds that could improve integration and enhance wound healing. These scaffolds, meticulously designed with a myriad of geometries, mechanical properties, and biological responses, are made possible through the vast selection of materials and fabrication methods at our disposal. Recognizing the importance of precision in the treatment of bone defects, which display variability from macroscopic to microscopic scales in each case, a tailored treatment strategy is required. A patient-specific AM bone scaffold perfectly addresses this necessity. This review elucidates the pivotal role that customized AM bone scaffolds play in bone defect treatment, while offering comprehensive guidelines for their customization. This includes aspects such as bone defect imaging, material selection, topography design, and fabrication methodology. Additionally, we propose a cooperative model involving the patient, clinician, and engineer, thereby underscoring the interdisciplinary approach necessary for the effective design and clinical application of these customized AM bone scaffolds. This collaboration promises to usher in a new era of bioactive medical materials, responsive to individualized needs and capable of pushing boundaries in personalized medicine beyond those set by traditional medical materials.

show abstract

“…However, several considerations, including complicated fabrication processes, preparation of multifaceted bioinks, application of supports that are unnecessary elements, and size of the spheroids, should be validated. Previously, we demonstrated a fabrication approach that combined cell spheroids with cell constructs using a microdroplet method and cell-loaded mineral oil [ 47 ]. In this study, mineral oil droplets were positioned within printed cell-laden bioinks, and the deposited cells exhibited self-assembly behavior after culturing and removing the oil components.…”

Section: Introductionmentioning

confidence: 99%