Hybrid identification for Glycine max and Glycine soja with SSR markers and analysis of salt tolerance

Abstract

Glycine max cultivars Lee68, Nannong 1138-2, and Nannong 8831 were used as the female parents, and hybrid lines (F5) 4,111, 4,076 (N23674 × BB52), 3,060 (Lee68 × N23227), and 185 (Jackson × BB52) that selected for salt tolerance generation by generation from the cross combination of G. max and G. soja were used as the male parents, 11 (A–K) backcrosses or three-way crosses were designed and 213 single hybrids were harvested. The optimized soybean simple sequence repeat (SSR)–polymerase chain reaction (PCR) system was used to analyze the SSR polymorphism of above parental lines and get the parental co-dominant SSR markers for hybrid identification, and in which 30 true hybrids were gained. The true hybrids (G1, G3, G9, G12, G13, G16) of G cross combination were chosen as the representative for the salt tolerance test, and the results showed that, as exposed to salt stress, the seedlings of G9 line displayed higher salt tolerant coefficient, relative growth rate, and dry matter accumulation, when compared with their female parent Nannong 1138-2, and even performed equally strong salt tolerance as the male parent 3,060. It provides a feasible method of the combination of molecular SSR markers and simple physiological parameters to identify the true hybrids of G. max and G. soja, and to innovate the salt-tolerant soybean germplasms.

Creation of new germplasm resources, development of SSR molecular markers and screen of monoterpene synthase in thyme

“…SSR molecular marker identi cation process is rstly to screen out codominant SSR primers in parents, and then to identify in progenies [40]. However, there may be errors in SSR molecular marker identi cation of hybrid progenies by using one pair of primers in practice.…”

“…Ampli cation products were analyzed by electrophoresis in 8.0% (w/v) denaturing polyacrylamide gel in TBE buffer for 1 h on the DYY-6C electrophoresis apparatus (Beijing Liuyi Instrument Factory, China) under 220 V constant voltage. Fragments were then visualized by silver staining (Silver sequence staining reagents, Promega, Madison, USA) and sized with 50 base pairs DNA ladder marker (Tiangen, China) [40]. SSR primer sequences were listed in Supplementary Table S2.…”

“…In this study, new germplasm resources of thyme were constructed by hybridizing thyme with different chemical types and plant types. SSR molecular marker are simple, time-consuming, reproducible and stable, which has been widely used in different species authenticity identi cation of hybrid progenies [15,40].…”

Development and characterization of 17 microsatellite markers for Sonchus oleraceus

“…Although chromosome numbers, morphological characteristics, and phylogenetic analysis provide some evidence to support this hypothesis, no corroborating results or conclusive evidence have been generated yet (Mejías and Andrés, 2004;Mejías et al, 2018). Therefore, in order to infer its phylogenetic divergence and expansion history on a local and global scale, it is of great importance to determine the origin of amphidiploid S. oleraceus.Given their high level of polymorphism, co-dominant inheritance patterns, and wide genomic distribution, microsatellites have been proven to be useful in estimating genetic diversity and characterizing population genetic structure, as well as for hybrid identification (Chen et al, 2017;Li et al, 2019). However, to our knowledge, no microsatellite markers have been developed for S. oleraceus or its closely related species despite their importance as agricultural weeds.…”

“…Given their high level of polymorphism, co‐dominant inheritance patterns, and wide genomic distribution, microsatellites have been proven to be useful in estimating genetic diversity and characterizing population genetic structure, as well as for hybrid identification (Chen et al., ; Li et al., ). However, to our knowledge, no microsatellite markers have been developed for S. oleraceus or its closely related species despite their importance as agricultural weeds.…”