Hybrid Male Sterility in Crosses Between Field and Laboratory Strains of Anopheles quadrimaculatus (Say) (Diptera: Culicidae)

Lanzaro, Gregory C.; Narang, S. K.; Mitchell, Sharon E.; Kaiser, P. E.; Seawright, J. A.

doi:10.1093/jmedent/25.4.248

Cited by 18 publications

(11 citation statements)

References 7 publications

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“…An average of one hundred mosquitoes were blood-fed on rabbits for 15 min the next day after field catches. Females were induced to oviposition, removing one of the wings using tweezers under a stereomicroscope and the placed individually in small plastic cups filled distilled water [24]. The next morning, females with more than one hundred eggs were used in the experiments and replicates comprised of 100 eggs from a single female.…”

Section: Methodsmentioning

confidence: 99%

Larval food quantity affects development time, survival and adult biological traits that influence the vectorial capacity of Anopheles darlingi under laboratory conditions

Araújo

Gil

e-Silva

2012

Malar J

118

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BackgroundThe incidence of malaria in the Amazon is seasonal and mosquito vectorial capacity parameters, including abundance and longevity, depend on quantitative and qualitative aspects of the larval diet. Anopheles darlingi is a major malaria vector in the Amazon, representing >95% of total Anopheles population present in the Porto Velho region. Despite its importance in the transmission of the Plasmodium parasite, knowledge of the larval biology and ecology is limited. Studies regarding aspects of adult population ecology are more common than studies on larval ecology. However, in order develop effective control strategies and laboratory breeding conditions for this species, more data on the factors affecting vector biology is needed. The aim of the present study is to assess the effects of larval food quantity on the vectorial capacity of An. darling under laboratory conditions.MethodsAnopheles darlingi was maintained at 28°C, 80% humidity and exposed to a daily photoperiod of 12 h. Larvae were divided into three experimental groups that were fed either a low, medium, or high food supply (based on the food amounts consumed by other species of culicids). Each experiment was replicated for six times. A cohort of adults were also exposed to each type of diet and assessed for several biological characteristics (e.g. longevity, bite frequency and survivorship), which were used to estimate the vectorial capacity of each experimental group.ResultsThe group supplied with higher food amounts observed a reduction in development time while larval survival increased. In addition to enhanced longevity, increasing larval food quantity was positively correlated with increasing frequency of bites, longer blood meal duration and wing length, resulting in greater vectorial capacity. However, females had greater longevity than males despite having smaller wings.ConclusionsOverall, several larval and adult biological traits were significantly affected by larval food availability. Greater larval food supply led to enhance larval and production and larger mosquitoes with longer longevity and higher biting frequency. Thus, larval food availability can alter important biological traits that influence the vectorial capacity of An. darlingi.

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Section: Methodsmentioning

confidence: 99%

Larval food quantity affects development time, survival and adult biological traits that influence the vectorial capacity of Anopheles darlingi under laboratory conditions

Araújo

Gil

e-Silva

2012

Malar J

118

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“…Mated females were placed into a screened 500-ml carton and provided a cotton plug wetted with a 10% sucrose solution. To induce oviposition, on the third day after mating, one wing was removed from each female, and they were placed individually in 50-ml plastic cups (Lanzaro et al 1988). After oviposition, the eggs were counted, each female was dissected, and the spermathecae examined for sperm by light microscopy.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Reproductive Isolation Between Sibling SpeciesAnopheles albitarsissensu stricto andAnopheles deaneorum, Two Malaria Vectors Belonging to the Albitarsis Complex (Diptera: Culicidae)

Lima

Valle

Peixoto³

2004

J Med Entomol

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Complexes of sibling species are common among mosquitoes, and their existence within vector species can have important epidemiological consequences. Anopheles albitarsis sensu stricto and Anopheles deaneorum Rosa-Freitas are two putative vectors of malaria parasites belonging to the Albitarsis species complex (Diptera: Culicidae). Using an induced mating technique, we studied the reproductive isolation between these two closely related species and their reciprocal hybrids. Evidence for hybrid male sterility consistent with Haldane's rule was found. The results indicate that male hybrids show very low insemination rates, probably due to abnormalities in their reproductive organs. In addition, the data show that hybrid males carrying an X chromosome derived from An. deaneorum perform significantly worse than hybrid males carrying an An. albitarsis s.s. X chromosome.

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“…In order to overcome the crepuscular oviposition, typical of An. albitarsis, some time points were collected according to the method described by Lanzara et al (1988). This method consists in the induction of oviposition through removal of one wing from females that have been blood fed four days before.…”

Section: Methodsmentioning

confidence: 99%

Anopheles albitarsis Embryogenesis: Morphological Identification of Major Events

Monnerat¹,

Pelajo-Machado²,

Vale³

et al. 2002

Mem. Inst. Oswaldo Cruz

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Hybrid Male Sterility in Crosses Between Field and Laboratory Strains of Anopheles quadrimaculatus (Say) (Diptera: Culicidae)

Cited by 18 publications

References 7 publications

Larval food quantity affects development time, survival and adult biological traits that influence the vectorial capacity of Anopheles darlingi under laboratory conditions

Larval food quantity affects development time, survival and adult biological traits that influence the vectorial capacity of Anopheles darlingi under laboratory conditions

Analysis of Reproductive Isolation Between Sibling SpeciesAnopheles albitarsissensu stricto andAnopheles deaneorum, Two Malaria Vectors Belonging to the Albitarsis Complex (Diptera: Culicidae)

Anopheles albitarsis Embryogenesis: Morphological Identification of Major Events

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