Hybrid microfluidic systems: combining a polymer microfluidic toolbox with biosensors

Gärtner, Claudia; Kirsch, Stefanie; Anton, Birgit; Becker, Holger

doi:10.1117/12.715041

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…optical). This technique can also be used to print sensors onto paper strips, forming screenprinted electrodes, and to integrate biomolecules in the electrodes paste, directly printing biomolecules for identification of specific analytes [10], [11]. Sensors fabricated with other technologies (spray-coating [12], coating coupled with embossing [13], photolithography and wet etching [14]) can also be irreversibly encapsulated into a microfluidic channel.…”

Section: Introductionmentioning

confidence: 99%

“…In this case the sensors are inserted inside the channel of the device through cavities [19], [20], which enables a re-use of the microfluidic system for different target molecules by allowing the introduction of different sensors. Nevertheless, in both approaches, one of the significant challenges is the integration between the sensor and the channel, which must be performed in order to avoid leakage at the interface [10]. Furthermore, when designing a microfluidic system, it is important to consider how the connection to external devices will be performed, both in terms of fluid handling and data or signal acquisition.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multi-function microfluidic platform for sensor integration

Fernandes

Semenova

Panjan³

et al. 2018

New Biotechnology

View full text Add to dashboard Cite

The limited availability of metabolite-specific sensors for continuous sampling and monitoring is one of the main bottlenecks contributing to failures in bioprocess development. Furthermore, only a limited number of approaches exist to connect currently available measurement systems with high throughput reactor units. This is especially relevant in the biocatalyst screening and characterization stage of process development. In this work, a strategy for sensor integration in microfluidic platforms is demonstrated, to address the need for rapid, cost-effective and high-throughput screening in bioprocesses. This platform is compatible with different sensor formats by enabling their replacement and was built in order to be highly flexible and thus suitable for a wide range of applications. Moreover, this re-usable platform can easily be connected to analytical equipment, such as HPLC, laboratory scale reactors or other microfluidic chips through the use of standardized fittings. In addition, the developed platform includes a two-sensor system interspersed with a mixing channel, which allows the detection of samples that might be outside the first sensor's range of detection, through dilution of the sample solution up to 10 times. In order to highlight the features of the proposed platform, inline monitoring of glucose levels is presented and discussed. Glucose was chosen due to its importance in biotechnology as a relevant substrate. The platform demonstrated continuous measurement of substrate solutions for up to 12 h. Furthermore, the influence of the fluid velocity on substrate diffusion was observed, indicating the need for in-flow calibration to achieve a good quantitative output.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multi-function microfluidic platform for sensor integration

Fernandes

Semenova

Panjan³

et al. 2018

New Biotechnology

View full text Add to dashboard Cite

show abstract

“…The use of stainless surgical needles [ 62 ] in fluidic connection has demonstrated good electrical contact or power connection for electrophoresis or isoelectric focusing applications [ 64 ]. For integrated on-chip electrode pads, electrical contacts can be deposited on the polymer surface by thermal or electron beam [ 64 , 65 ], screen printing [ 66 ], or 3D ion implantation electrode [ 67 ]. Many microfluidic devices are analyzed by optical detection.…”

Section: Polymer Microfluidics Fabrication Proceduresmentioning

confidence: 99%

Polymer Microfluidics: Simple, Low-Cost Fabrication Process Bridging Academic Lab Research to Commercialized Production

2016

View full text Add to dashboard Cite

Using polymer materials to fabricate microfluidic devices provides simple, cost effective, and disposal advantages for both lab-on-a-chip (LOC) devices and micro total analysis systems (μTAS). Polydimethylsiloxane (PDMS) elastomer and thermoplastics are the two major polymer materials used in microfluidics. The fabrication of PDMS and thermoplastic microfluidic device can be categorized as front-end polymer microchannel fabrication and post-end microfluidic bonding procedures, respectively. PDMS and thermoplastic materials each have unique advantages and their use is indispensable in polymer microfluidics. Therefore, the proper selection of polymer microfabrication is necessary for the successful application of microfluidics. In this paper, we give a short overview of polymer microfabrication methods for microfluidics and discuss current challenges and future opportunities for research in polymer microfluidics fabrication. We summarize standard approaches, as well as state-of-art polymer microfluidic fabrication methods. Currently, the polymer microfluidic device is at the stage of technology transition from research labs to commercial production. Thus, critical consideration is also required with respect to the commercialization aspects of fabricating polymer microfluidics. This article provides easy-to-understand illustrations and targets to assist the research community in selecting proper polymer microfabrication strategies in microfluidics.

show abstract

“…microdevices, and thereby decrease the possible environmental impact that an intense use of this technology might bring. Table 2 -Materials used for microfluidic platforms and their main characteristics (Mark et al, 2010), (Wu and Gu, 2011a), (Nge et al, 2013), (Iliescu et al, 2012), (Li et al, 2012), (Kangning Ren, Jianhua Zhou, 2013), (Gärtner et al, 2007), (Martinez et al, 2010), (Liana et al, 2012), (Yetisen et al, 2013), (Xia et al, 2016).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“Connecting worlds – a view on microfluidics for a wider application”

Fernandes

Gernaey

Krühne

2018

Biotechnology Advances

View full text Add to dashboard Cite

From its birth, microfluidics has been referenced as a revolutionary technology and the solution to long standing technological and sociological issues, such as detection of dilute compounds and personalized healthcare. Microfluidics has for example been envisioned as: (1) being capable of miniaturizing industrial production plants, thereby increasing their automation and operational safety at low cost; (2) being able to identify rare diseases by running bioanalytics directly on the patient's skin; (3) allowing health diagnostics in point-of-care sites through cheap lab-on-a-chip devices. However, the current state of microfluidics, although technologically advanced, has so far failed to reach the originally promised widespread use. In this paper, some of the aspects are identified and discussed that have prevented microfluidics from reaching its full potential, especially in the chemical engineering and biotechnology fields, focusing mainly on the specialization on a single target of most microfluidic devices and offering a perspective on the alternate, multi-use, "plug and play" approach. Increasing the flexibility of microfluidic platforms, by increasing their compatibility with different substrates, reactions and operation conditions, and other microfluidic systems is indeed of surmount importance and current academic and industrial approaches to modular microfluidics are presented. Furthermore, two views on the commercialization of plug-and-play microfluidics systems, leading towards improved acceptance and more widespread use, are introduced. A brief review of the main materials and fabrication strategies used in these fields, is also presented. Finally, a step-wise guide towards the development of microfluidic systems is introduced with special focus on the integration of sensors in microfluidics. The proposed guidelines are then applied for the development of two different example platforms, and to three examples taken from literature. With this work, we aim to provide an interesting perspective on the field of microfluidics when applied to chemical engineering and biotechnology studies, as well as to contribute with potential solutions to some of its current challenges.

show abstract

Hybrid microfluidic systems: combining a polymer microfluidic toolbox with biosensors

Cited by 7 publications

References 0 publications

Multi-function microfluidic platform for sensor integration

Multi-function microfluidic platform for sensor integration

Polymer Microfluidics: Simple, Low-Cost Fabrication Process Bridging Academic Lab Research to Commercialized Production

“Connecting worlds – a view on microfluidics for a wider application”

Contact Info

Product

Resources

About