Hybrid Monoliths for Magnetically‐Driven Protein Separations

Abstract

Monoliths represent powerful platforms for isolation of large molecules with high added value. This work presents a hybrid approach for antibody (Ab) capture and release. Using mostly natural polymers and clean processes, it is possible to create macroporous monoliths with well‐defined porous networks, tuneable mechanical properties, and easy functionalization with a biomimetic ligand specific for Ab. Magnetic nanoparticles (MNPs) are embedded on the monolith network to confer a controlled magnetic response that facilitates and accelerates Ab recovery in the elution step. The hybrid monolithic systems prepared with agarose or chitosan/poly(vinyl alcohol) (PVA) blends exhibit promising binding capacities of Abs directly from cell‐culture extracts (120 ± 10 mg Ab g−1 support) and controlled Ab magnetically‐assisted elution yielding 95 ± 2% recovery. Moreover, a selective capture of mAbs directly from cell culture extracts is achieved yielding a final mAb preparation with 96% of purity.

Nanohybrid polymer brushes on silica for bioseparation

“…Organic/inorganic hybrid materials have attracted ever increasing attention in recent years due to their unique structural characteristics and potential applications in bioseparation, 1 targeted drug/ gene delivery, 2,3 theranostics and more. 4 Typically, hybrid particles are composed of inorganic cores and surrounding organic layers in the form of polymer shells or polymer brushes.…”

Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage‐display

“…This has been previously reported for other affinity systems, as the elution conditions when using cross‐linked agarose or magnetic particles for the same affinity ligand and target are not always the same and often require case‐to‐case optimization (e.g. ).…”

Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

“…The earlier works aimed at optimizing the polymerization conditions, the content of precursors (functional monomer and crosslinker), as well as the type and composition of porogens to be essential for controlling the porous structures of polymeric monoliths . Several approaches were then developed, including adjustment of phase separation, decreasing polymerization time, using either single crosslinker or a few unusual crosslinkers as precursors, post‐hypercrosslinking via Friedel–Crafts alkylation reaction, and incorporating nanoparticles (e.g., gold, C60 Fullerene, carbon nanotube, metal–organic framework, magnetic nanoparticles, silver, and single‐walled carbon nanohorns) into the monoliths.…”