Hybrid Nanoscale Architecture for Enhancement of Antimicrobial Activity: Immobilization of Silver Nanoparticles on Thiol‐Functionalized Polymer Crystallized on Carbon Nanotubes

Abstract

We describe here the synthesis and antimicrobial activity of an innovative nanohybrid system, characterized by attachment of silver nanoparticles (AgNPs) to the thiol-functionalized polymer that was periodically crystallized on carbon nanotubes (CNTs). The synthesis of the nanohybrid architecture first involved direct crystallization of thiol-functionalized copolymer along the long axis of CNTs, followed by attachment of AgNPs to the thiol-group of functionalized copolymer. The antimicrobial activity was assessed in terms of interaction with Escherichia coli, where the constituents of the nanohybrid structure play a synergistic role. The antimicrobial activity was approximately four orders of magnitude greater than the ex situ precipitated bare AgNPs. Possible mechanisms underlying enhanced antimicrobial activity are discussed. The study underscores the potential of uniquely combining CNTs and biopolymers for biomedical applications, in the present case, antimicrobial activity. Fig. 7. Schematic illustration of release of silver ions from bare AgNPs and polymer-CNT/Ag hybrid structure and their antimicrobial behavior.

Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches

“…Physical and chemical properties of AgNPs—including surface chemistry, size, size distribution, shape, particle morphology, particle composition, coating/capping, agglomeration, dissolution rate, particle reactivity in solution, efficiency of ion release, cell type, and finally type of reducing agents used for synthesis—are crucial factors for determination of cytotoxicity [ 15 , 50 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 ]. For example, using biological reducing agents such as culture supernatants of various Bacillus species, AgNPs can be synthesized in various shapes, such as spherical, rod, octagonal, hexagonal, triangle, flower-like, and so on ( Figure 2 ).…”

Tunable Nanometer‐Scale Architecture of Organic–Inorganic Hybrid Nanostructured Materials for Structural and Functional Applications

“…This nanometer-scale hybrid architecture with silver nanoparticles anchored to the hybrid structure is benefi cial in enhancing and favorably modulating antimicrobial activity by providing physical stability to nanoparticles and in the release of silver ions for interaction with E. coli . [ 28 ] Furthermore, the antimicrobial activity attributable to silver nanoparticles anchored to the thiol group of the polymer can be controlled by tuning the spacing between the lamellae polymer crystals, as illustrated in Figure 2 .…”

Carbon nanohorns allow acceleration of osteoblast differentiationviamacrophage activation

“…[2][3][4][5][6][7][8][9][10][11] Carbon nanomaterials are promising candidates for bone tissue engineering applications due to their superior cytocompatible, mechanical and electrical properties. [12][13][14][15][16][17][18] Some years ago, we initiated to explore study the applications of carbon nanomaterials for bone tissue regeneration. We have reported that CNT-coated substrates can be effective for the adhesion and differentiation of osteoblasts, while CNT-coated collagen sponges resulted to possess a favorable biocompatibility profile with bone.…”