Hybrid nanostructured gadolinium oxide-collagen-dextran polymeric hydrogel for corneal repair and regeneration

Vijayan, Vinu; Kira, Manikantan Syamala

doi:10.1016/j.ijbiomac.2022.10.229

Cited by 10 publications

(3 citation statements)

References 70 publications

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“…Thus, in AO/PI dual staining assay, alive cells appear in green color and dead cells in yellow‐orange to red color depending upon the stage of apoptosis occurring in cells. [ 52 ] The results showed only negligible numbers of cells were exhibiting yellow to orange fluorescence. Most of the cells were fluorescing in green color indicating the viability of these cells.…”

Section: Resultsmentioning

confidence: 99%

Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ‐Carrageenan Hydroxyapatite Biocomposite as Angio‐Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair

et al. 2023

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A composite biomatrix fabricated with collagen, ƙ‐carrageenan, hydroxyapatite reinforced with lanthanum oxide nanoparticles is explored as proangiogenic and osteogenic bone tissue repair biomaterial. The biomatrix shows increased physical and biological stability as observed from proteolytic degradation and thermal stability studies. The addition of lanthanum oxide nanoparticles facilitates good osseointegration coupled with simultaneous activation of proangiogenic properties to act as a bone mimicking material. The minimal level of reactive oxygen species and superior cytocompatibility help the as‐synthesized biomatrix in achieving capillary migration into the bone micro environment. The composite biomatrix upregulates the expression of VEGF, VEGF‐R2 genes in endothelial cells and osteopontin, osteocalcin in osteoblasts cells, respectively. The in vivo hard tissue repair experiment conducted in a rat model shows complete healing of the bone defect by eight weeks with the application of collagen‐ƙ‐carrageenan‐hydroxyapatite‐lanthanum oxide nanoparticle biomaterial when compared to the biomaterial made out of individual constituents alone. The biomaterial matrix gets biointegrated into the bone tissue and exerts its therapeutic value in bringing a faster osseo repair process. The study shows the feasibility of using rare‐earth metal nanoparticles in combination with protein‐polysaccharide biopolymers for bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ‐Carrageenan Hydroxyapatite Biocomposite as Angio‐Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair

et al. 2023

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“…Titanium-based biocompatible surfaces are critical in tissue engineering, osteogenesis and bacterial adhesion, as well as in implantable organic and smart electronics devices [1][2][3][4][5][6][7][8][9][10][11][12]. With the aim to include antimicrobial properties in non-biodegradable implants, selective molecular or polymeric coatings along with antibacterial elements have been used for the manufacturing of medical device surfaces [13][14][15][16][17][18]. Anticancer L-glutamine green corrosion inhibitor is bonded on antibacterial Cu (111) suitable for coating on Ti-based implants [13], while antibacterial polyurethanes exhibit appropriate mechanical properties and hemocompatibility [14].…”

Section: Introductionmentioning

confidence: 99%

Electronic and Structural Properties of Antibacterial Ag–Ti-Based Surfaces: An Ab Initio Theoretical Study

Papantoniou-Chatzigiosis,

Galani,

Fylaktopoylou

et al. 2024

Crystals

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Coatings with tunable multifunctional features are important for several technological applications. Ti-based materials have been used in diverse applications ranging from metallic diodes in electronic devices up to medical implants. This work uses ab initio calculations to achieve a more fundamental understanding of the structural and electronic properties of β-TiNb and its passive TiO2 film surfaces upon Ag addition, investigating the alterations in the electronic band gap and the stability of the antibacterial coating. We find that Ag’s 4d electrons introduce localized electron states, characterized by bonding features with the favoured Ti first neighbour atoms, approximately −5 eV below the fermi level in both β-TiNb bulk and surface. Ag’s binding energy on β-TiNb(110) depends on the local environment (the lattice site and the type of bonded surface atoms) ranging from −2.70 eV up to −4.21 eV for the adatom on a four-fold Ti site, offering a variety of options for the design of a stable coating or for Ag ion release. In Ti–O terminated anatase and rutile (001) surfaces, surface states are introduced altering the TiO2 band gap. Silver is bonded more strongly, and therefore creates a more stable antibacterial coat on rutile than on anatase. In addition, the Ag coating exhibits enhanced 4d electron states at the highest occupied state on anatase (001),which are extended from −5 eV up to the Fermi level on rutile (001), which might be altered depending on the coat structural features, thus creating systems with tunable electronic band gap that can be used for the design of thin film semiconductors.

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“…Phalloidin Staining: The cell attachment and spreading on the fabricated bio-matrix was studied by staining the polymerized actin filaments (F-actin) using phalloidin. [52] The cells were seeded on the bio-matrices and incubated at 37 °C. After 24 hours, the cells were fixed using 4% formaldehyde for 15 minutes at room temperature, permeabilized using permeabilization buffer containing 0.3% Triton X-100 in PBS for 10 minutes, and stained with Phalloidin-FITC for 30 minutes in the dark.…”

mentioning

confidence: 99%

Caffeine‐reinforced Collagen as Localized Microenvironmental Trans‐Browning Bio‐Matrix for Soft Tissue Repair and Regeneration in Bariatric Condition

Sreekumar,

Vijayan,

Gangaraj

et al. 2023

Advanced Biology

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The wound exudates, hypoperfusion of the subcutaneous fat layer, and poor vasculature worsen wound management in obese subjects. In the current study, a multifunctional Caffeine‐reinforced collagen biomaterial is developed that can simultaneously modulate lipid metabolism and angiogenesis in obese wound microenvironments for faster tissue regeneration. The biomaterial is fabricated specialized for obese conditions to initiate simultaneous lipolysis and angiogenesis locally in the hypoxic subcutaneous fat in wound margins of obese subjects. Caffeine‐reinforced collagen biomatrix shows better structural integrity, thermal stability, bio‐compatibility, and lesser proteolytic susceptibility. Caffeine‐collagen biomaterial promote angiogenesis, fibroblast migration, and localized browning of white adipocytes to activate thermogenesis in the subcutaneous fat layer at the wound site. Full‐thickness excision wound healing studies performed in obese C57BL6 mice shows faster wound closure within day 9 when compare to control mice. The Caffeine‐reinforced collagen biomaterial remodeled the wound site locally by activating fibroblast to secrete collagen, activate endothelial cells to promote angiogenesis, and induce browning in white adipocytes in subcutaneous fat. The study opens a new direction in bariatric tissue regenerative medicine by locally modulating lipid metabolism, angiogenesis, and trans‐browning at the injured site for faster complete restoration of the damaged tissue.

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Hybrid nanostructured gadolinium oxide-collagen-dextran polymeric hydrogel for corneal repair and regeneration

Cited by 10 publications

References 70 publications

Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ‐Carrageenan Hydroxyapatite Biocomposite as Angio‐Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair

Lanthanum Oxide Nanoparticles Reinforced Collagen ƙ‐Carrageenan Hydroxyapatite Biocomposite as Angio‐Osteogenic Biomaterial for In Vivo Osseointegration and Bone Repair

Electronic and Structural Properties of Antibacterial Ag–Ti-Based Surfaces: An Ab Initio Theoretical Study

Caffeine‐reinforced Collagen as Localized Microenvironmental Trans‐Browning Bio‐Matrix for Soft Tissue Repair and Regeneration in Bariatric Condition

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