Hybrid nonlinear photoacoustic and reflectance confocal microscopy for label-free subcellular imaging with a single light source

Mattison, Scott P.; Mondragón, Eli; Kaunas, Roland; Applegate, Brian E.

doi:10.1364/ol.42.004028

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…Although nonlinear RSA materials are anticipated to be the most promising dye category for generation of an enhanced nonlinear PA response it must be kept in mind that their application is very much limited to scenarios where the application of high laser fluences is feasible. While a number of studies addressing nonlinear PAI have emerged very few have involved in vivo studies , none of which have relied solely on a molecular‐based contrast agent. As such, all of the in vivo imaging applications of MPACs discussed in this review have been conducted under low laser fluence conditions where the dye either behaves as a LA or SA material.…”

Section: Classes Of Photoacoustic Contrast Agentsmentioning

confidence: 99%

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Borg

Rochford

2018

Photochem & Photobiology

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Photoacoustic imaging (PAI) is a rapidly growing field which offers high spatial resolution and high contrast for deep-tissue imaging in vivo. PAI is nonionizing and noninvasive and combines the optical resolution of fluorescence imaging with the spatial resolution of ultrasound imaging. In particular, the development of exogenous PA contrast agents has gained significant momentum of late with a vastly expanding complexity of dye materials under investigation ranging from small molecules to macromolecular proteins, polymeric and inorganic nanoparticles. The goal of this review is to survey the current state of the art in molecular photoacoustic contrast agents (MPACs) for applications in biomedical imaging. The fundamental design principles of MPACs are presented and a review of prior reports spanning from early-to-current literature is put forth.

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Section: Classes Of Photoacoustic Contrast Agentsmentioning

confidence: 99%

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Borg

Rochford

2018

Photochem & Photobiology

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“…Other nonlinear mechanisms have also been explored to achieve sub-diffraction resolutions in PAM, including transient optical absorption (88,89), optical absorption saturation (2,11), and thermal relaxation (7,90). All these methods share the same goal-nonlinear PA signal generation, and can be applied to any samples.…”

Section: Super-resolution Pat (Sr-pat)-mentioning

confidence: 99%

Listening to tissues with new light: recent technological advances in photoacoustic imaging

Razansky

Yao

2019

J. Opt.

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Photoacoustic tomography (PAT), or optoacoustic tomography, has achieved remarkable progress in the past decade, benefiting from the joint developments in optics, acoustics, chemistry, computing and mathematics. Unlike pure optical or ultrasound imaging, PAT can provide unique optical absorption contrast as well as widely scalable spatial resolution, penetration depth and imaging speed. Moreover, PAT has inherent sensitivity to tissue's functional, molecular, and metabolic state. With these merits, PAT has been applied in a wide range of life science disciplines, and has enabled biomedical research unattainable by other imaging methods. This Review article aims at introducing state-of-the-art PAT technologies and their representative applications. The focus is on recent technological breakthroughs in structural, functional, molecular PAT, including super-resolution imaging, real-time small-animal whole-body imaging, and high-sensitivity functional/molecular imaging. We also discuss the remaining challenges in PAT and envisioned opportunities.

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Integrated photoacoustic and hyperspectral dual‐modality microscopy for co‐imaging of melanoma and cutaneous squamous cell carcinoma in vivo

Liu

Chen

Xing

2020

Journal of Biophotonics

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Skin carcinoma such as melanoma (MM) and cutaneous squamous cell carcinoma (cSCC) are considered as the highest mortality and the most aggressive skin cancers in dermatology. In view that early diagnosis and treatment can greatly improve the survival rate and life quality of the patients, developing noninvasive and effective evaluation methods is of great significance for the detection and identification of early stage cutaneous cancers. In this article, we propose a hybrid photoacoustic and hyperspectral dual‐modality microscopy to evaluate and differentiate skin carcinoma by structural and multiphysiological parameters. The proposed system's imaging abilities are verified by mimic phantoms and normal mice experiments. Furthermore, in vivo characterization and evaluation results of MM and cSCC mice are obtained successfully, which prove this novel method could be used as a reliable and useful method for skin cancer detection in early stages.

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Hybrid nonlinear photoacoustic and reflectance confocal microscopy for label-free subcellular imaging with a single light source

Cited by 6 publications

References 23 publications

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Listening to tissues with new light: recent technological advances in photoacoustic imaging

Integrated photoacoustic and hyperspectral dual‐modality microscopy for co‐imaging of melanoma and cutaneous squamous cell carcinoma in vivo

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