Hybrid opto-electric manipulation in microfluidics—opportunities and challenges

Kumar, Aloke; Williams, Stuart J.; Chuang, Huey-Ru; Green, Nicolas G.; Wereley, Steven T.

doi:10.1039/c1lc20208a

Cited by 57 publications

(48 citation statements)

References 106 publications

(176 reference statements)

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“…REP is an optoelectrokinetic technique, excellent in micro/ nano particle manipulation. [29][30][31][32]40 The microchip for REP requires no complicated fabrication but only two parallel plate electrodes separated by a spacer. Successful particle concentration occurs when laser irradiation and an electric field are simultaneously applied on the microchip.…”

Section: Discussionmentioning

confidence: 99%

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A bead-based fluorescence immunosensing technique enabled by the integration of Förster resonance energy transfer and optoelectrokinetic concentration

Wang

Shieh

et al. 2016

Biomicrofluidics

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Bead-based immunosensing has been growing as a promising technology in the point-of-care diagnostics due to great flexibility. For dilute samples, functionalized particles can be used to collect dispersed analytes and act as carriers for particle manipulation. To realize rapid and visual immunosensing, F€ orster resonance energy transfer (FRET) was used herein to ensure only the diabetic biomarker, lipocalin 1, to be detected. The measurement was made in an aqueous droplet sandwiched between two parallel plate electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the FRET fluorescent signal. The optoelectrokinetic technique, termed rapid electrokinetic patterning (REP), has been proven to be excellent in dynamic and programmable particle manipulation. Therefore, the detection can be complete within several tens of seconds. The lower detection limit of the REP-enabled bead-based diagnosis reached nearly 5 nM. The combinative use of FRET and the optoelectrokinetic technique for the bead-based immunosensing enables a rapid measure to diagnose early stage diseases and dilute analytes. V C 2016 AIP Publishing LLC. [http://dx

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Section: Discussionmentioning

confidence: 99%

“…The attempt successfully demonstrated such an integrated system for a sensitive detection. Later, an optoelectrokinetic technique, termed rapid electrokinetic patterning (REP), [29][30][31][32] has also been developed to implement intricate particle manipulation. Programmability enables REP to effectively concentrate dilute analytes.…”

Section: Introductionmentioning

confidence: 99%

A bead-based fluorescence immunosensing technique enabled by the integration of Förster resonance energy transfer and optoelectrokinetic concentration

Wang

Shieh

et al. 2016

Biomicrofluidics

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“…Dynamic or programmable manipulation can be achieved by a DMD [18,33,34], a projector [35,36], or a liquid-crystalbased spatial light modulator [37,38]. Additional optical components, including an objective lens, are usually needed to focus light patterns on to the photosensitive layer to obtain a resolution of 1-20 μm [19].…”

Section: Optically Induced Electrokinetic (Oek) Forces 45mentioning

confidence: 99%

“…Apart from this spatial light modulation through a predefined mask, researchers and engineers have also proposed various devices for flexible modulation purposes, such as programmable digital micro-mirror devices (DMDs), MEMS mirror arrays, and liquid-crystal-based spatial light modulators. In the areas of micro-and nanoscale manipulation, a major question concerns how one could introduce light waves into conventional methods to endow them with flexibility, noninvasive properties, dynamic capabilities, a higher degree of freedom [19], and achieve high resolution and high throughput at the same time. In this chapter, we introduce a manipulation technology that is based on combining optical and electrokinetic principles -more specifically, optically induced electrokinetics (OEK) and its Q1 applications in manipulation and assembly of micro-and nanoscale entities.…”

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confidence: 99%

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Lai

et al. 2015

Micro‐ and Nanomanipulation Tools

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IntroductionIn the past 25 years, integrated circuit (IC) and microelectromechanical systems (MEMSs) technologies have moved much beyond the microdomain, and into the submicro-, nanoscopic, and, even, the molecular scales. Today, we have the capability to fabricate, manipulate, and assemble matters at the micro-, nano-, or molecular scales. This progress into "seeing" and manipulating matters that are smaller than visible light wavelength scale is impacting a range of fields including semiconductor physics, biological studies, pharmaceutical development, and many other scientific and technological applications. A range of new methods to generate physical forces have been developed in the process. For example, mechanical forces can now be applied to micro-, nano-, and biological entities using atomic force microscope probes [1], microgrippers [2], or pipettes [3]. However, disadvantages of these approaches, including inflection of mechanical damages on objects being manipulated and their relatively low manipulation speed, make manipulation of large quantities of objects difficult. In order to address these disadvantages, noncontact or noninvasive methods for micro-, nano-, and biological manipulation have also been explored in the past two decades.Manipulation devices based on magnetic forces [4], acoustic waves [5,6], hydrodynamic flows [7], light waves [8], or electrokinetic forces [9] are among the major noninvasive technologies available today. Each of them has its own advantages and disadvantages. Methods based on magnetic fields need premodification on objects with magnetic beads which constrains their application domain. In addition, movement controlled by permanent magnets is not precise enough for many proposed applications. Also, the high resistance of the magnetic coil generates significant heat during manipulation [10]. Acoustic traps show good selectivity but cannot achieve parallel manipulation of single entities. Microfluidic devices exploiting hydrodynamic flows are suitable for cell population sorting, but have difficulties in trapping or controlling specific single objects.Electrokinetic forces generated from patterned electrodes capable of inducing the desired spatial distribution of electric field have been exploited to control

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“…Although there are many studies in the literature regarding the based bio-particle manipulation with different techniques, there are few studies regarding the integrated and/or hybrid implementation of these techniques which include the hybrid use of magnetophoresis and DEP, 18,19 optics and DEP, 20,21 optics and ACP, 22 and hydrophoresis and DEP. 24 Regarding the integration and/or hybrid use of ACP with DEP, the number of studies are also limited.…”

Section: Introductionmentioning

confidence: 99%

An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining

et al. 2016

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In this study, acoustophoresis and dielectrophoresis are utilized in an integrated manner to combine the two different operations on a single polydimethylsiloxane (PDMS) chip in sequential manner, namely, particle wash (buffer exchange) and particle separation. In the washing step, particles are washed with buffer solution with low conductivity for dielectrophoretic based separation to avoid the adverse effects of Joule heating. Acoustic waves generated by piezoelectric material are utilized for washing, which creates standing waves along the whole width of the channel. Coupled electro-mechanical acoustic 3D multi-physics analysis showed that the position and orientation of the piezoelectric actuators are critical for successful operation. A unique mold is designed for the precise alignment of the piezoelectric materials and 3D side-wall electrodes for a highly reproducible fabrication. To achieve the throughput matching of acoustophoresis and dielectrophoresis in the integration, 3D side-wall electrodes are used. The integrated device is fabricated by PDMS molding. The mold of the integrated device is fabricated using high-precision mechanical machining. With a unique mold design, the placements of the two piezoelectric materials and the 3D sidewall electrodes are accomplished during the molding process. It is shown that the proposed device can handle the wash and dielectrophoretic separation successfully. V C 2016 AIP Publishing LLC.

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Hybrid opto-electric manipulation in microfluidics—opportunities and challenges

Cited by 57 publications

References 106 publications

A bead-based fluorescence immunosensing technique enabled by the integration of Förster resonance energy transfer and optoelectrokinetic concentration

A bead-based fluorescence immunosensing technique enabled by the integration of Förster resonance energy transfer and optoelectrokinetic concentration

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

An integrated acoustic and dielectrophoretic particle manipulation in a microfluidic device for particle wash and separation fabricated by mechanical machining

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